Microarray analysis of differentially expressed genes in L929 mouse fibroblast cells exposed to leptin and hypoxia

Objective: Unlike traditional composite resins, bulk-fill composite resins could be polymerized as thicker layers. This study aims to contribute to the field by investigating the cytotoxic effects of various bulk-fill composite resins on L929 mouse fibroblast cells in vitro. Material and Methods: In our study, six bulk fill and one conventional composite resin were used. Composite resin samples (8×4 mm) were prepared in a sterile cabinet by using a glass mod and polymerizing with a led light device (DTE LUX E, Germany). Composite samples (n:3) of which surface area was calculated according to ISO 10993-12: 2012 standards (3 cm2/ml), were kept in media for 24 h and 72 h in 37 oC incubator, their extracts were filtered in 1:1 and 1:2 proportion and were added on L929 mouse fibroblast cells. Cell viability was examined by the MTT assay and cell death by the LDH test. Cell viability results were evaluated using one-way analysis of variance (ANOVA) test (p<0.05). Results: When the 1:1 extracts from 4 mm thick bulk-fill composite samples were applied on L929 mouse fibroblast cells, cell viability rates showed significant differences compared to the control group at the end of 24 h and 72 h (except for Estelite Bulk Fill Flow). Although the extracts of the tested composite samples at 1:1 and 1:2 ratio at the end of 72 hours caused a decrease in L929 mouse fibroblast cell viability, the cell viability rate of only PRG-containing bulk fill composite and conventional composite remained below the cell viability ratio (70%) specified in ISO standards. Bulk fill composites did not produce toxic effects (except Beautifil Bulk Restorative) according to the LDH test. Conclusions: Despite decreasing in general the cell viability, bulk-fill composite resins used in 4 mm thick layers provided cell viability rates over the acceptability level, except PRG-containing bulk fill composite (Beautifil Bulk Restorative), which was cytotoxic to L929 mouse fibroblasts. Keywords Bulk fill composite; Cytotoxicity; L929 cells; LDH assay.

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A nano chitosan membrane barrier prepared via Nanospider technology with non-toxic solvent for peritoneal adhesions’ prevention

Journal of Biomaterials Applications ◽

10.1177/08853282211008109 ◽

2021 ◽

pp. 088532822110081

Author(s):

Shuo Zhang ◽

Zhuoyue Xu ◽

Xuejun Wen ◽

Changzheng Wei

Keyword(s):

Mouse Fibroblast ◽

Electrospun Membrane ◽

Adhesion Barrier ◽

Peritoneal Adhesions ◽

Elongation At Break ◽

In Vivo Experiments ◽

L929 Mouse Fibroblast ◽

Membrane Barrier ◽

L929 Mouse

Peritoneal adhesion is one of the most common postsurgical complications and can cause bowel obstruction, pelvic pain, and infertility. Setting up a physical barrier directly between the injured site and surrounding tissues is an effective solution for preventing this adverse situation. This study investigated a chitosan electrospun membrane (CSEM) as a potent anti-adhesion barrier, which was prepared by a needleless technology called Nanospider. Scanning electron microscopy revealed that CSEM is a laminated nanofiber with good mechanical properties. The fiber is uniform with the diameter distributing in the range of 100–120 nm. The tensile strength can reach 27.45 ± 6.30 MPa with a maximum elongation at break of 18.50 ± 1.44%, which makes it stick easily to damaged parts but not to be easily damaged by tissue friction. The growth of S. aureus on CSEM was 59.18% lower than the control at 10 h, which indicates its better antibacterial property. In addition, CSEM has good coagulant and biocompatibility characteristics. It can perform hemostatic function within 10 min and the L929 mouse fibroblast viability on it was 92.18% ± 1.08% on the seventh day. In vivo experiments indicated that CSEM significantly prevented peritoneal adhesions within four weeks after surgery with wound surface coverage. These results indicate that CSEM is a promising anti-adhesion barrier material.

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Identification of differentially expressed genes in human pineal parenchymal tumors by microarray analysis

Acta Neuropathologica ◽

10.1007/s00401-004-0964-6 ◽

2004 ◽

Vol 109 (3) ◽

pp. 306-313 ◽

Cited By ~ 7

Author(s):

Jacques Champier ◽

Anne Jouvet ◽

Catherine Rey ◽

Virginie Brun ◽

Arlette Bernard ◽

...

Keyword(s):

Differentially Expressed Genes ◽

Microarray Analysis ◽

Differentially Expressed ◽

Pineal Parenchymal Tumors

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A new series of 2,4-thiazolidinediones endowed with potent aldose reductase inhibitory activity

Open Chemistry ◽

10.1515/chem-2021-0032 ◽

2021 ◽

Vol 19 (1) ◽

pp. 347-357

Author(s):

Belgin Sever ◽

Mehlika Dilek Altıntop ◽

Yeliz Demir ◽

Cüneyt Türkeş ◽

Kaan Özbaş ◽

...

Keyword(s):

Aldose Reductase ◽

In Silico ◽

Aromatic Aldehydes ◽

Docking Studies ◽

Mouse Fibroblast ◽

L929 Mouse Fibroblast ◽

Orally Bioavailable ◽

L929 Mouse ◽

Solvent Free Reaction

Abstract In an effort to identify potent aldose reductase (AR) inhibitors, 5-(arylidene)thiazolidine-2,4-diones (1–8), which were prepared by the solvent-free reaction of 2,4-thiazolidinedione with aromatic aldehydes in the presence of urea, were examined for their in vitro AR inhibitory activities and cytotoxicity. 5-(2-Hydroxy-3-methylbenzylidene)thiazolidine-2,4-dione (3) was the most potent AR inhibitor in this series, exerting uncompetitive inhibition with a K i value of 0.445 ± 0.013 µM. The IC50 value of compound 3 for L929 mouse fibroblast cells was determined as 8.9 ± 0.66 µM, pointing out its safety as an AR inhibitor. Molecular docking studies suggested that compound 3 exhibited good affinity to the binding site of AR (PDB ID: 4JIR). Based upon in silico absorption, distribution, metabolism, and excretion data, the compound is predicted to have favorable pharmacokinetic features. Taking into account the in silico and in vitro data, compound 3 stands out as a potential orally bioavailable AR inhibitor for the management of diabetic complications as well as nondiabetic diseases.

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