electrospun membrane
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2022 ◽  
Vol Volume 17 ◽  
pp. 17-29
Author(s):  
Xue Gao ◽  
Mohammed A Al-Baadani ◽  
Minjie Wu ◽  
Ningyang Tong ◽  
Xinkun Shen ◽  
...  
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Author(s):  
Soyoung Hong ◽  
Yejin Song ◽  
Jaesoon Choi ◽  
Changmo Hwang

Abstract BACKGROUND: In vitro generation of three-dimensional vessel network is crucial to investigate and possibly improve vascularization after implantation in vivo. This work has the purpose of engineering complex tissue regeneration of a vascular network including multiple cell-type, an extracellular matrix, and perfusability for clinical application. METHODS: The two electrospun membranes bonded with the vascular network shape are cultured with endothelial cells and medium flow through the engineered vascular network. The flexible membranes are bonded by amine-epoxy reaction and examined the perfusability with fluorescent beads. Also, the perfusion culture for 7 days of the endothelial cells is compared with static culture on the engineered vascular network membrane. RESULTS: The engineered membranes are showed perfusability through the vascular network, and the perfused network resulted in more cell proliferation and variation of the shear stress-related genes expression compared to the static culture. Also, for the generation of the complex vascularized network, pericytes are co-cultured with the engineered vascular network, which results in the Collagen I is expressed on the outer surface of the engineered structure. CONCLUSION: This study is showing the perfusable in vitro engineered vascular network with electrospun membrane. In further, the 3D vascularized network module can be expected as a platform for drug screening and regenerative medicine.



Energies ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 7123
Author(s):  
Tomasz N. Kołtunowicz ◽  
Piotr Gałaszkiewicz ◽  
Konrad Kierczyński ◽  
Przemysław Rogalski ◽  
Paweł Okal ◽  
...  

The paper examined Ti3C2Tx MXene (T—OH, Cl or F), which is prepared by etching a layered ternary carbide Ti3AlC2 (312 MAX-phase) precursor and deposited on a polycaprolactone (PCL) electrospun membrane (MXene-PCL nanocomposite). X-ray Diffraction analysis (XRD) and Scanning Electron Microscopy (SEM) indicates that the obtained material is pure Ti3C2 MXene. SEM of the PCL-MXene composite demonstrate random Ti3C2 distribution over the nanoporous membrane. Results of capacitance, inductance, and phase shift angle studies of the MXene-PCL nanocomposite are presented. It was found that the frequency dependence of the capacitance exhibited a clear sharp minima in the frequency range of 50 Hz to over 104 Hz. The frequency dependence of the inductance shows sharp maxima, the position of which exactly coincides with the position of the minima for the capacitance, which indicates the occurrence of parallel resonances. Current conduction occurs by electron tunneling between nanoparticles. In the frequency range from about 104 Hz to about 105 Hz, there is a broad minimum on the inductance relationship. The position of this minimum coincides exactly with the position of the maximum of the phase shift angle—its amplitude is close to 90°. The real value of the inductance of the nanocomposite layer was determined to be about 1 H. It was found that the average value of the distance over which the electron tunnels was determined with some approximation to be about 5.7 nm and the expected value of the relaxation time to be τM ≈ 3 × 10−5 s.



2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yan Cui ◽  
Zongwang Huang ◽  
Li Lei ◽  
Qinglin Li ◽  
Jinlong Jiang ◽  
...  

AbstractDeath from acute hemorrhage is a major problem in military conflicts, traffic accidents, and surgical procedures, et al. Achieving rapid effective hemostasis for pre-hospital care is essential to save lives in massive bleeding. An ideal hemostasis material should have those features such as safe, efficient, convenient, economical, which remains challenging and most of them cannot be achieved at the same time. In this work, we report a rapid effective nanoclay-based hemostatic membranes with nanoclay particles incorporate into polyvinylpyrrolidone (PVP) electrospun fibers. The nanoclay electrospun membrane (NEM) with 60 wt% kaolinite (KEM1.5) shows better and faster hemostatic performance in vitro and in vivo with good biocompatibility compared with most other NEMs and clay-based hemostats, benefiting from its enriched hemostatic functional sites, robust fluffy framework, and hydrophilic surface. The robust hemostatic bandages based on nanoclay electrospun membrane is an effective candidate hemostat in practical application.



2021 ◽  
Vol 421 ◽  
pp. 129621
Author(s):  
Shengyang Zheng ◽  
Manhong Huang ◽  
Songmei Sun ◽  
Haitao Zhao ◽  
Lijun Meng ◽  
...  


Author(s):  
Abdullrahman M. Al-Bishari ◽  
Kendrick Hii Ru Yie ◽  
Mohammed A. Al-Baadani ◽  
Bilal A. Al-Shaaobi ◽  
Zixin Zhou ◽  
...  




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