scholarly journals Cytotoxic Effects of Bulk-Fill Composites on L929 Fibroblast Cells

2021 ◽  
Vol 24 (1) ◽  
Author(s):  
Numan Aydın ◽  
Serpil Karaoğlanoğlu ◽  
Elif Aybala Oktay ◽  
Aysun Kılıç Süloğlu
Keyword(s):  
Cell Viability ◽  
Composite Resin ◽  
Mouse Fibroblast ◽  
Composite Resins ◽  
Control Group ◽  
Fibroblast Cells ◽  
Cytotoxic Effects ◽  
L929 Mouse Fibroblast ◽  
L929 Mouse ◽  
Composite Samples

Objective: Unlike traditional composite resins, bulk-fill composite resins could be polymerized as thicker layers. This study aims to contribute to the field by investigating the cytotoxic effects of various bulk-fill composite resins on L929 mouse fibroblast cells in vitro. Material and Methods: In our study, six bulk fill and one conventional composite resin were used. Composite resin samples (8×4 mm) were prepared in a sterile cabinet by using a glass mod and polymerizing with a led light device (DTE LUX E, Germany). Composite samples (n:3) of which surface area was calculated according to ISO 10993-12: 2012 standards (3 cm2/ml), were kept in media for 24 h and 72 h in 37 oC incubator, their extracts were filtered in 1:1 and 1:2 proportion and were added on L929 mouse fibroblast cells. Cell viability was examined by the MTT assay and cell death by the LDH test. Cell viability results were evaluated using one-way analysis of variance (ANOVA) test (p<0.05). Results: When the 1:1 extracts from 4 mm thick bulk-fill composite samples were applied on L929 mouse fibroblast cells, cell viability rates showed significant differences compared to the control group at the end of 24 h and 72 h (except for Estelite Bulk Fill Flow). Although the extracts of the tested composite samples at 1:1 and 1:2 ratio at the end of 72 hours caused a decrease in L929 mouse fibroblast cell viability, the cell viability rate of only PRG-containing bulk fill composite and conventional composite remained below the cell viability ratio (70%) specified in ISO standards. Bulk fill composites did not produce toxic effects (except Beautifil Bulk Restorative) according to the LDH test. Conclusions: Despite decreasing in general the cell viability, bulk-fill composite resins used in 4 mm thick layers provided cell viability rates over the acceptability level, except PRG-containing bulk fill composite (Beautifil Bulk Restorative), which was cytotoxic to L929 mouse fibroblasts. Keywords Bulk fill composite; Cytotoxicity; L929 cells; LDH assay.

scholarly journals In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells

2021 ◽  
Vol 25(5) (25(5)) ◽  
pp. 608-617
Author(s):  
Rosa Mhlanga CHINHEYA ◽  
Murat YILMAZ ◽  
Aylin ÜSTÜNDAĞ ◽  
Seda İPEK ◽  
Yalçın DUYDU ◽  
...  
Keyword(s):  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
Genotoxic Effects ◽  
Pulp Capping ◽  
L929 Mouse Fibroblast ◽  
In Vitro Investigation ◽  
L929 Mouse

scholarly journals A Preliminary Research Study on the Cytotoxicity of Expired and Non-expired Composite Resins: In Vitro Study

2020 ◽  
pp. 227-238
Author(s):  
Magrur Kazak DDS, PhD ◽  
Nazmiye Donmez DDS, PhD ◽  
Fatemeh Bahadori PhD ◽  
Vildan Betul Yenigun PhD ◽  
Abdurrahim Kocyigit MD, PhD
Keyword(s):  
Cell Viability ◽  
Composite Resin ◽  
In Vitro Study ◽  
Negative Control Group ◽  
Negative Control ◽  
Composite Resins ◽  
Control Group ◽  
Significant Difference ◽  
Preliminary Research

Objective: Studies have focused on use of non-expired composites. Unfortunately some clinicians still use expired composite resins without considering their effects. The objective of this in vitro preliminary research was to investigate cytotoxicity of expired(6-months) and non-expired composite resins. Materials and methods: Expired (E) and non-expired (NE) samples of one bulk-fill (Tetric N-Ceram Bulk-fill [TNB], Ivoclar Vivadent), two nano-hybrid (Tetric N-Ceram [TN], Ivoclar Vivadent; Clearfil Majesty ES-2 [CM], Kuraray) composite resins were tested on L929 fibroblast cells. Medium covering cells was removed then plastic rings (2-mm height) were filled with non-polymerized composite resins, placed in direct contact with cells and polymerized with LED light curing unit (LCU). Three samples were prepared for each group. After polymerization, removed medium was added to the cells. Cells that were left without medium (WOM) and cells that were exposed to LCU were used as positive control groups. Cells without any treatment were used as negative control group (C). Cells were incubated with tested materials for 7-days to evaluate cytotoxicity. Cell viability was calculated by sulforhodamine B test as a percentage (%). One-way ANOVA and post-hoc Tukey tests were used for statistical analyses (p<0.05). Results: Comparison between E and NE groups of same composite resins did not result in statistically significant differences (p>0.05), except between TN NE and TN E (p<0.05). TN E group was significantly more cytotoxic than TN NE group. When NE composite resin groups were compared to each other, statistically significant difference was only obtained between TNB NE and TN NE (p<0.05). Among all tested groups, TN NE group showed the least cytotoxic profile. No statistically significant differences were determined when E composite resin groups were compared to each other (p>0.05). All experimental groups compared with C group showed statistically significant cytotoxicity (p<0.05). A statistically significant difference existed between LCU and C groups (p<0.05). Conclusions: In clinical practice, expired composite resins should never be used. Although a correlation was found between expiration dates of nano-hybrid composite resins and cell viability, opposite data were obtained for bulk-fill composite resin. Researches are still required to evaluate biocompatibility of bulk-fill composite resins at various thicknesses with current LCUs.

GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells

Biochimie ◽  
2019 ◽  
Vol 162 ◽  
pp. 88-96
Author(s):  
Lauren E. Rylaarsdam ◽  
Grace N. Johnecheck ◽  
Brendan D. Looyenga ◽  
Larry L. Louters
Keyword(s):  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
L929 Mouse Fibroblast ◽  
L929 Mouse

scholarly journals Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells

2014 ◽  
Vol 33 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Neda NAGHAVI ◽  
Jamileh GHODDUSI ◽  
Hamid R. SADEGHNIA ◽  
Elham ASADPOUR ◽  
Saeed ASGARY
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Mouse Fibroblast ◽  
Fibroblast Cells ◽  
L929 Mouse Fibroblast ◽  
L929 Mouse

scholarly journals Cytotoxicity of latex and non-latex orthodontic elastomeric ligatures on L929 mouse fibroblasts

2010 ◽  
Vol 21 (3) ◽  
pp. 205-210 ◽  
Author(s):  
Rogério Lacerda dos Santos ◽  
Matheus Melo Pithon ◽  
Fernanda Otaviano Martins ◽  
Maria Teresa Villela Romanos ◽  
Antônio Carlos de Oliveira Ruellas
Keyword(s):  
Cell Viability ◽  
Fibroblast Cell ◽  
Neutral Red ◽  
Mouse Fibroblast ◽  
Fibroblast Cell Line ◽  
Dye Uptake ◽  
L929 Mouse Fibroblast ◽  
Mouse Fibroblast Cell Line ◽  
Red Dye ◽  
L929 Mouse

This study investigated the cytotoxicity exists between latex and non-latex Orthodontic elastomeric ligatures. Six elastomeric ligatures (1 latex, 2 latex-free and 3 polyurethane) from different manufacturers were divided into 6 groups of 15 elastics each: A (Latex-free, American Orthodontics), M (Polyurethane, Morelli), G (Polyurethane,GAC International), Te (Polyurethane, Tecnident), TP (Natural latex,TP Orthodontics) and U (Latex-free,3M Unitek). The cytotoxicity assay was performed using cell cultures (L929 mouse fibroblast cell line), which were subjected to the cell viability test with neutral red ("dye-uptake") at 1, 2, 3, 7 and 28 days. Data were analyzed statistically by ANOVA and Tukey's test (α=0.05). No statistically significant differences (p>0.05) were observed between Groups M and Te in all experimental periods, except at 2 days. No significant differences (p>0.05) in cell viability were found either among Groups A, G, TP and U or between Groups M and Te at 24 h or among Groups CC, A, G, TP and U at 2 and 28 days. It may be concluded that latex-free elastomeric ligatures from American Orthodontics and Unitek trademarks induced less cell lysis compared to latex and polyurethane ligatures.

Effect of layer thickness on residual monomer release in polymerization of bulk-fill composites

2021 ◽  
pp. 096739112199958
Author(s):  
Vahti Kılıç ◽  
Feridun Hurmuzlu ◽  
Yılmaz Ugur ◽  
Suzan Cangul
Keyword(s):  
Stainless Steel ◽  
Layer Thickness ◽  
Composite Resin ◽  
Ethanol Solution ◽  
Composite Resins ◽  
Residual Monomer ◽  
Steel Mold ◽  
Nanohybrid Composite ◽  
Composite Samples

The aim of the present study was to investigate and compare the quantity of residual monomers leached from the bulk-fill composites with different compositions polymerized at varying layer thickness. Three bulk-fill (X-tra-fil, Beautifil Bulk Restorative, Fill-Up) and a nanohybrid composite (Filtek Z550) were used for the study. The composite resin samples were prepared with a stainless steel mold. For each composite, two groups were constructed. The samples in the first group were prepared using the 2 + 2 mm layering technique. In the second group, the composite samples were applied as a 4 mm-thick one layer and polymerized. Then, each composite samples were kept in a 75% ethanol solution and residual monomers released from composite resins were analyzed with an HPLC device after 24hour and 1 month. The data were analyzed using Kruskal-Wallis and Mann-Whitney U tests. Except the Fill-Up, all of residual monomer elution from the bulk-fill composites was significantly affected by the layer thickness (p < 0.05). The greatest monomer release was detected at 1 month after polymerization as a single 4 mm layer for Beautifil Bulk Restorative. Fill-Up composite showed similar residual monomer release in polymerization at different layer thicknesses compared to other composite resins. In the 2 + 2 mm layering technique, the least monomer elution was detected in the Filtek Z550 composite group. While Bis-GMA was the most released monomer in X-tra fil composite, UDMA was the most released monomer in all other composite resins. During polymerization of the bulk-fill composite, the layer thickness of the composite applied may affect the amount of residual monomers released from the composite resins. Conventional composites may release less monomer than bulk-fill composites when used with layering.

A nano chitosan membrane barrier prepared via Nanospider technology with non-toxic solvent for peritoneal adhesions’ prevention

2021 ◽  
pp. 088532822110081
Author(s):  
Shuo Zhang ◽  
Zhuoyue Xu ◽  
Xuejun Wen ◽  
Changzheng Wei
Keyword(s):  
Mouse Fibroblast ◽  
Electrospun Membrane ◽  
Adhesion Barrier ◽  
Peritoneal Adhesions ◽  
Elongation At Break ◽  
In Vivo Experiments ◽  
L929 Mouse Fibroblast ◽  
Membrane Barrier ◽  
L929 Mouse

Peritoneal adhesion is one of the most common postsurgical complications and can cause bowel obstruction, pelvic pain, and infertility. Setting up a physical barrier directly between the injured site and surrounding tissues is an effective solution for preventing this adverse situation. This study investigated a chitosan electrospun membrane (CSEM) as a potent anti-adhesion barrier, which was prepared by a needleless technology called Nanospider. Scanning electron microscopy revealed that CSEM is a laminated nanofiber with good mechanical properties. The fiber is uniform with the diameter distributing in the range of 100–120 nm. The tensile strength can reach 27.45 ± 6.30 MPa with a maximum elongation at break of 18.50 ± 1.44%, which makes it stick easily to damaged parts but not to be easily damaged by tissue friction. The growth of S. aureus on CSEM was 59.18% lower than the control at 10 h, which indicates its better antibacterial property. In addition, CSEM has good coagulant and biocompatibility characteristics. It can perform hemostatic function within 10 min and the L929 mouse fibroblast viability on it was 92.18% ± 1.08% on the seventh day. In vivo experiments indicated that CSEM significantly prevented peritoneal adhesions within four weeks after surgery with wound surface coverage. These results indicate that CSEM is a promising anti-adhesion barrier material.

Influence of Hydrogen Peroxide Bleaching Gels on Color, Opacity, and Fluorescence of Composite Resins

2012 ◽  
Vol 37 (5) ◽  
pp. 526-531 ◽  
Author(s):  
CRG Torres ◽  
CF Ribeiro ◽  
E Bresciani ◽  
AB Borges
Keyword(s):  
Hydrogen Peroxide ◽  
Composite Resin ◽  
Color Change ◽  
Composite Resins ◽  
Control Group ◽  
Peroxide Bleaching ◽  
Color Changes ◽  
Tukey Test ◽  
Bleaching Gels ◽  
Bleaching Treatment

SUMMARY The aim of the present study was to evaluate the effect of 20% and 35% hydrogen peroxide bleaching gels on the color, opacity, and fluorescence of composite resins. Seven composite resin brands were tested and 30 specimens, 3-mm in diameter and 2-mm thick, of each material were fabricated, for a total of 210 specimens. The specimens of each tested material were divided into three subgroups (n=10) according to the bleaching therapy tested: 20% hydrogen peroxide gel, 35% hydroxide peroxide gel, and the control group. The baseline color, opacity, and fluorescence were assessed by spectrophotometry. Four 30-minute bleaching gel applications, two hours in total, were performed. The control group did not receive bleaching treatment and was stored in deionized water. Final assessments were performed, and data were analyzed by two-way analysis of variance and Tukey tests (p&lt;0.05). Color changes were significant for different tested bleaching therapies (p&lt;0.0001), with the greatest color change observed for 35% hydrogen peroxide gel. No difference in opacity was detected for all analyzed parameters. Fluorescence changes were influenced by composite resin brand (p&lt;0.0001) and bleaching therapy (p=0.0016) used. No significant differences in fluorescence between different bleaching gel concentrations were detected by Tukey test. The greatest fluorescence alteration was detected on the brand Z350. It was concluded that 35% hydrogen peroxide bleaching gel generated the greatest color change among all evaluated materials. No statistical opacity changes were detected for all tested variables, and significant fluorescence changes were dependent on the material and bleaching therapy, regardless of the gel concentration.

scholarly journals A new series of 2,4-thiazolidinediones endowed with potent aldose reductase inhibitory activity

2021 ◽  
Vol 19 (1) ◽  
pp. 347-357
Author(s):  
Belgin Sever ◽  
Mehlika Dilek Altıntop ◽  
Yeliz Demir ◽  
Cüneyt Türkeş ◽  
Kaan Özbaş ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
In Silico ◽  
Aromatic Aldehydes ◽  
Docking Studies ◽  
Mouse Fibroblast ◽  
L929 Mouse Fibroblast ◽  
Orally Bioavailable ◽  
L929 Mouse ◽  
Solvent Free Reaction

Abstract In an effort to identify potent aldose reductase (AR) inhibitors, 5-(arylidene)thiazolidine-2,4-diones (1–8), which were prepared by the solvent-free reaction of 2,4-thiazolidinedione with aromatic aldehydes in the presence of urea, were examined for their in vitro AR inhibitory activities and cytotoxicity. 5-(2-Hydroxy-3-methylbenzylidene)thiazolidine-2,4-dione (3) was the most potent AR inhibitor in this series, exerting uncompetitive inhibition with a K i value of 0.445 ± 0.013 µM. The IC50 value of compound 3 for L929 mouse fibroblast cells was determined as 8.9 ± 0.66 µM, pointing out its safety as an AR inhibitor. Molecular docking studies suggested that compound 3 exhibited good affinity to the binding site of AR (PDB ID: 4JIR). Based upon in silico absorption, distribution, metabolism, and excretion data, the compound is predicted to have favorable pharmacokinetic features. Taking into account the in silico and in vitro data, compound 3 stands out as a potential orally bioavailable AR inhibitor for the management of diabetic complications as well as nondiabetic diseases.

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