scholarly journals First records of the fanged frogs Limnonectes bannaensis Ye, Fei & Jiang, 2007 and L. utara Matsui, Belabut & Ahmad, 2014 (Amphibia: Anura: Dicroglossidae) in Thailand

2021 ◽  
Vol 9 ◽  
Author(s):  
Chatmongkon Suwannapoom ◽  
Ke Jiang ◽  
Yun-He Wu ◽  
Parinya Pawangkhanant ◽  
Sengvilay Lorphengsy ◽  
...  
Keyword(s):  
Cryptic Species ◽  
Species Complex ◽  
Sequence Data ◽  
Taxonomic Status ◽  
Morphological Data ◽  
Integrative Approach ◽  
Species Identity ◽  
Dna Sequence Data ◽  
Species Complexes ◽  
Cryptic Species Complex

The taxonomic status of the Thai populations belonging to the Limnonectes kuhlii species complex is controversial, due to phenotypic similarity in the cryptic species complex. Recently, some studies on this group in Thailand have discovered four new species: L. taylori, L. megastomias, L. jarujini and L. isanensis. Even so, the diversity of this group is still incomplete. Based on an integrative approach encompassing genetic and morphological analyses, we conclude that the Limnonectes populations from Nan Province (northern) and Yala Province (southern) of Thailand are conspecific with L. bannaensis Ye, Fei & Jiang, 2007 and L. utara Matsui, Belabut & Ahmad, 2014, respectively. These are the first records of these species in Thailand. Our study highlights the importance of using DNA sequence data in combination with morphological data to accurately document species identity and diversity. This is especially important for morphologically cryptic species complexes and sympatrically occurring congeners.

scholarly journals Veterinary Fusarioses within the United States

2016 ◽  
Vol 54 (11) ◽  
pp. 2813-2819 ◽  
Author(s):  
Kerry O'Donnell ◽  
Deanna A. Sutton ◽  
Nathan Wiederhold ◽  
Vincent A. R. G. Robert ◽  
Pedro W. Crous ◽  
...  
Keyword(s):  
United States ◽  
Dna Sequence ◽  
Species Complex ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Distinct Species ◽  
The United States ◽  
Content Type ◽  
Dna Sequence Data ◽  
Species Complexes

Multilocus DNA sequence data were used to assess the genetic diversity and evolutionary relationships of 67Fusariumstrains from veterinary sources, most of which were from the United States. Molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. The majority of the veterinary isolates (47/67 = 70.1%) were nested within theFusarium solanispecies complex (FSSC), and these included 8 phylospecies and 33 unique 3-locus sequence types (STs). Three of the FSSC species (Fusarium falciforme,Fusarium keratoplasticum, andFusariumsp. FSSC 12) accounted for four-fifths of the veterinary strains (38/47) and STs (27/33) within this clade. Most of theF. falciformestrains (12/15) were recovered from equine keratitis infections; however, strains ofF. keratoplasticumandFusariumsp. FSSC 12 were mostly (25/27) isolated from marine vertebrates and invertebrates. Our sampling suggests that theFusarium incarnatum-equisetispecies complex (FIESC), with eight mycoses-associated species, may represent the second most important clade of veterinary relevance withinFusarium. Six of the multilocus STs within the FSSC (3+4-eee, 1-b, 12-a, 12-b, 12-f, and 12-h) and one each within the FIESC (1-a) and theFusarium oxysporumspecies complex (ST-33) were widespread geographically, including three STs with transoceanic disjunctions. In conclusion, fusaria associated with veterinary mycoses are phylogenetically diverse and typically can only be identified to the species level using DNA sequence data from portions of one or more informative genes.

scholarly journals Molecular and anatomical analyses reveal that Peronia verruculata (Gastropoda: Onchidiidae) is a cryptic species complex

2018 ◽  
Vol 87 (3) ◽  
pp. 149-165 ◽  
Author(s):  
Jia Jin Marc Chang ◽  
Ywee Chieh Tay ◽  
Hui Ping Ang ◽  
Karenne Phyu Phyu Tun ◽  
Loke Ming Chou ◽  
...  
Keyword(s):  
Cryptic Species ◽  
Dna Sequence ◽  
Sequence Data ◽  
Accessory Gland ◽  
Sequence Information ◽  
Dna Sequence Data ◽  
Distinct Sequence ◽  
Cryptic Species Complex ◽  
Taxonomic Approach ◽  
Intertidal Environments

Recent years have seen an increase in the number of studies that use DNA sequence information in addition to morphological methods, as the latter alone can be inadequate for morphologically similar, cryptic species. Marine onchidiid slugs (Mollusca: Gastropoda: Onchidiidae), which commonly inhabit intertidal environments and have a near-global distribution, comprise 11 genera and 86 described species. Singapore has 19 recorded species of onchidiids with Peronia verruculata (Cuvier, 1830) being the most abundant. Here we present mitochondrial DNA sequence data from 63 P. verruculata-like specimens to show that they constitute two closely-related clades with distinct sequence signatures and a clear barcode gap. Intraclade distances are 0–1.8% while interclade distances range from 4.7 to 6.0%. Scanning electron microscopy of internal copulation organs reveals that the two clades have distinct penial and accessory gland spine structures, which also differ in size. Using an integrative taxonomic approach, we propose that what was initially thought to be P. verruculata in Singapore, actually are two different species of Peronia.

Phylogeny of the Crepidula plana (Gastropoda: Calyptraeidae) cryptic species complex in North America

2000 ◽  
Vol 78 (8) ◽  
pp. 1500-1514 ◽  
Author(s):  
Rachel Collin
Keyword(s):  
North Carolina ◽  
North America ◽  
Species Complex ◽  
East Coast ◽  
Sequence Data ◽  
Single Species ◽  
The United States ◽  
Molecular Techniques ◽  
Dna Sequence Data ◽  
Cryptic Species Complex

The taxonomy of Crepidula species with flat white shells is particularly difficult. These animals from the east coast of North America have generally been classified as a single species, Crepidula plana Say, 1822. Based on allozyme and developmental data, however, Hoagland (K.E. Hoagland. 1984. Malacologia, 25: 607-628; K.E. Hoagland. 1986. Am. Malacol. Bull. 4: 173-183) concluded that two species of flat white-shelled Crepidula live along the east coast of the United States, but she did not apply any name to the second species. Herein I use molecular techniques to characterize populations of flat white-shelled Crepidula species from Texas, Florida, Georgia, North Carolina, and |Massachusetts, and describe their morphology and development. DNA-sequence data support the existence of three species. One species is readily distinguished on the basis of morphology and development, but the other two are very similar. To clarify the nomenclature of these species, I designate neotypes for C. plana Say, 1822 and Crepidula depressa Say, 1822, and describe Crepidula atrasolea sp.nov.

scholarly journals An integrative approach to unravel the Ceratitis FAR (Diptera, Tephritidae) cryptic species complex: a review

ZooKeys ◽  
2015 ◽  
Vol 540 ◽  
pp. 405-427 ◽  
Author(s):  
Marc de Meyer ◽  
Hélène Delatte ◽  
Sunday Ekesi ◽  
Kurt Jordaens ◽  
Blanka Kalinova ◽  
...  
Keyword(s):  
Cryptic Species ◽  
Species Complex ◽  
Integrative Approach ◽  
Cryptic Species Complex

Description of Three Pristionchus Species (Nematoda: Diplogastridae) from Japan that Form a Cryptic Species Complex with the Model Organism P. pacificus

2012 ◽  
Vol 29 (6) ◽  
pp. 403 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Erik J. Ragsdale ◽  
Matthias Herrmann ◽  
Werner E. Mayer ◽  
Ralf J. Sommer
Keyword(s):  
Cryptic Species ◽  
Species Complex ◽  
Model Organism ◽  
Cryptic Species Complex

FUSARIUM-ID v.3.0: An updated, downloadable resource for Fusarium species identification

Plant Disease ◽  
2021 ◽  
Author(s):  
Terry Torres-Cruz ◽  
Briana Whitaker ◽  
Robert Proctor ◽  
Kirk Broders ◽  
Imane Laraba ◽  
...  
Keyword(s):  
Sequence Data ◽  
Safety Concern ◽  
Fusarium Species ◽  
Elongation Factor ◽  
Sequence Database ◽  
Accurate Identification ◽  
Dna Sequence Data ◽  
Species Complexes ◽  
Marker Loci ◽  
Feed Safety

Species within Fusarium are of global agricultural, medical, and food/feed safety concern and have been extensively characterized. However, accurate identification of species is challenging and usually requires DNA sequence data. FUSARIUM-ID (http://isolate.fusariumdb.org/) is a publicly available database designed to support the identification of Fusarium species using sequences of multiple phylogenetically informative loci, especially the highly informative ~680 bp 5' portion of the translation elongation factor 1-alpha (TEF1) gene that has been adopted as the primary barcoding locus in the genus. However, FUSARIUM-ID v.1.0 and 2.0 had several limitations, including inconsistent metadata annotation for the archived sequences and poor representation of some species complexes and marker loci. Here, we present FUSARIUM-ID v.3.0, which provides the following improvements: (i) additional and updated annotation of metadata for isolates associated with each sequence, (ii) expanded taxon representation in the TEF1 sequence database, (iii) availability of the sequence database as a downloadable file to enable local BLAST queries, and (iv) a tutorial file for users to perform local BLAST searches using either freely-available software, such as SequenceServer, BLAST+ executable in the command line, and Galaxy, or the proprietary Geneious software. FUSARIUM-ID will be updated on a regular basis by archiving sequences of TEF1 and other loci from newly identified species and greater in-depth sampling of currently recognized species.

scholarly journals An integrative approach to discovering cryptic species within the Bemisia tabaci whitefly species complex

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Soňa Vyskočilová ◽  
Wee Tek Tay ◽  
Sharon van Brunschot ◽  
Susan Seal ◽  
John Colvin
Keyword(s):  
Cryptic Species ◽  
Bemisia Tabaci ◽  
Species Complex ◽  
Integrative Approach

Molecular phylogeny and systematics of the centipede genus Ethmostigmus Pocock (Chilopoda : Scolopendromorpha) from peninsular India

2018 ◽  
Vol 32 (6) ◽  
pp. 1316 ◽  
Author(s):  
Jahnavi Joshi ◽  
Gregory D. Edgecombe
Keyword(s):  
Dna Sequence ◽  
Molecular Phylogenetics ◽  
Sequence Data ◽  
Indian Plate ◽  
Eastern Ghats ◽  
Integrative Approach ◽  
Phylogenetic Position ◽  
Ecological Data ◽  
Peninsular India ◽  
Dna Sequence Data

Integrative taxonomy assesses the congruence between different lines of evidence for delimiting species, such as morphological, molecular or ecological data. Herein molecular phylogenetics is used to test monophyly and determine the phylogenetic position of the Old World tropical centipede genus Ethmostigmus Pocock, 1898, and to define species boundaries for Ethmostigmus in peninsular India. A phylogeny of the family Scolopendridae based on DNA sequence data for three markers from 427 specimens sampling in all major lineages (144 individuals generated in this study) recovers Ethmostigmus as a monophyletic group, but relationships among the genera in its subfamily Otostigminae are poorly supported. Two species delimitation methods for DNA sequence data and phylogeny are integrated with morphology and geographic data to propose a well-supported species hypothesis for Ethmostigmus on the peninsular Indian plate. Five species of Ethmostigmus are recognised in peninsular India, of which E. coonooranus Chamberlin, 1920 and three new species, namely, E. agasthyamalaiensis, sp. nov., E. sahyadrensis, sp. nov. and E. praveeni, sp. nov., occur in the Western Ghats, a biodiversity hotspot. The lesser-known Eastern Ghats harbour one species, E. tristis (Meinert, 1886), which has been nearly unreported for 130 years. This study highlights the value of an integrative approach to systematics, especially in underexplored, high biodiversity regions and where morphological variation is limited among closely related species.

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