Comparative FISH-mapping of TTAGG telomeric sequences to the chromosomes of leafcutter ants (Formicidae, Myrmicinae): is the insect canonical sequence conserved?

Telomeric sequences are conserved across species. The most common sequence reported among insects is (TTAGG)n, but its universal occurrence is not a consensus because other canonical motifs have been reported. In the present study, we used fluorescence in situ hybridization (FISH) using telomeric probes with (TTAGG)6 repeats to describe the telomere composition of leafcutter ants. We performed the molecular cytogenetic characterization of six Acromyrmex Mayr, 1865 and one Atta Fabricius, 1804 species (Acromyrmex ambiguus (Emery, 1888), Ac. crassispinus (Forel, 1909), Ac. lundii (Guérin-Mèneville, 1838), Ac. nigrosetosus (Forel, 1908), Ac. rugosus (Smith, 1858), Ac. subterraneus subterraneus (Forel, 1893), and Atta sexdens (Linnaeus, 1758)) and described it using a karyomorphometric approach on their chromosomes. The diploid chromosome number 2n = 38 was found in all Acromyrmex species, and the karyotypic formulas were as follows: Ac. ambiguus 2K = 14M + 12SM + 8ST + 4A, Ac. crassispinus 2K = 12M + 20SM + 4ST + 2A, Ac. lundii 2K = 10M + 14SM + 10ST + 4A, Ac. nigrosetosus 2K = 12M + 14SM + 10ST + 2A, and Ac. subterraneus subterraneus 2K = 14M + 18SM + 4ST + 2A. The exact karyotypic formula was not established for Ac. rugosus. FISH analyses revealed the telomeric regions in all the chromosomes of the species studied in the present work were marked by the (TTAGG)6 sequence. These results reinforce the premise that Formicidae presents high homology between their genera for the presence of the canonical sequence (TTAGG)n.

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Molecular Cytogenetic Characterization of the DiGeorge Syndrome Region Using Fluorescence in Situ Hybridization

Genomics ◽

10.1006/geno.1993.1339 ◽

1993 ◽

Vol 17 (2) ◽

pp. 403-407 ◽

Cited By ~ 60

Author(s):

Elizabeth A. Lindsay ◽

Stephanie Halford ◽

Roy Wadey ◽

Peter J. Scambler ◽

Antonio Baldini

Keyword(s):

In Situ Hybridization ◽

Fluorescence In Situ Hybridization ◽

Digeorge Syndrome ◽

Molecular Cytogenetic ◽

Cytogenetic Characterization

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Molecular cytogenetic characterization of the KG-1 and KG-1a acute myeloid leukemia cell lines by use of spectral karyotyping and fluorescence in situ hybridization

Genes Chromosomes and Cancer ◽

10.1002/gcc.10274 ◽

2003 ◽

Vol 38 (3) ◽

pp. 249-252 ◽

Cited By ~ 13

Author(s):

Krzysztof Mrózek ◽

Stephan M. Tanner ◽

Kristiina Heinonen ◽

Clara D. Bloomfield

Keyword(s):

Myeloid Leukemia ◽

Leukemia Cell ◽

Spectral Karyotyping ◽

Molecular Cytogenetic ◽

Leukemia Cell Lines ◽

Cytogenetic Characterization ◽

Acute Myeloid ◽

Myeloid Leukemia Cell

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Establishment and molecular cytogenetic characterization of non–small cell lung cancer cell line KU-T1 by multicolor fluorescence in situ hybridization, comparative genomic hybridization, and chromosome microdissection

Cancer Genetics and Cytogenetics ◽

10.1016/j.cancergencyto.2007.07.020 ◽

2007 ◽

Vol 179 (2) ◽

pp. 93-101 ◽

Cited By ~ 2

Author(s):

Motohiko Kume ◽

Takahiro Taguchi ◽

Hironobu Okada ◽

Takashi Anayama ◽

Akira Tominaga ◽

...

Keyword(s):

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

Comparative Genomic ◽

Chromosome Microdissection ◽

Lung Cancer Cell ◽

Molecular Cytogenetic ◽

Cytogenetic Characterization ◽

Multicolor Fluorescence

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Molecular Cytogenetic Characterization of a Non-Robertsonian Dicentric Chromosome 14;19 Identified in a Girl with Short Stature and Amenorrhea

Case Reports in Genetics ◽

10.1155/2012/212065 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5

Author(s):

Usha R. Dutta ◽

Vijaya Kumar Pidugu ◽

Ashwin Dalal

Keyword(s):

Short Stature ◽

Cytogenetic Analysis ◽

Clinical Phenotype ◽

Chromosome Rearrangement ◽

Dicentric Chromosome ◽

Derivative Chromosome ◽

Molecular Cytogenetic ◽

Cytogenetic Characterization

We report a 16-year-old girl who presented with short stature and amenorrhea. Initially the cytogenetic analysis showed the presence of a mosaic non-Robertsonian dicentric chromosome involving chromosomes 14 and 19. Subsequent molecular cytogenetic analysis by fluorescencein situhybridization (FISH) using whole chromosome paints, centromeric probes, as well as gene specific probes confirmed the dicentric nature of the derivative chromosome and indicated that the rearrangement involved the short arms of both of these chromosomes. Furthermore, we also determined that the chromosome 19p13.3 breakpoint occurred within the terminal 1 Mb region. This is the first report of a mosaic non-Robertsonian dicentric chromosome involving chromosomes 14 and 19 with the karyotype determined as 45,XX,dic(14;19)(p11.2;p13.3)[35]/46,XX[15], and we suggest that the chromosome rearrangement could be the cause of clinical phenotype.

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Clinical and molecular cytogenetic characterization of two cases of inverted duplication deletion 8p

Nauchno-prakticheskii zhurnal «Medicinskaia genetika» ◽

10.25557/2073-7998.2020.03.41-42 ◽

2020 ◽

pp. 41-42

Author(s):

Д.А. Юрченко ◽

М.Е. Миньженкова ◽

Е.Л. Дадали ◽

Н.В. Шилова

Keyword(s):

Mental Retardation ◽

Congenital Heart ◽

Heart Defects ◽

Clinical Manifestations ◽

Formation Mechanisms ◽

Agenesis Of Corpus Callosum ◽

Molecular Cytogenetic ◽

Inverted Duplication ◽

Cytogenetic Characterization

Синдром инвертированной дупликации короткого плеча хромосомы 8 со смежной терминальной делециенй (inv dup del(8p), ORPHA 96092) - редкая хромосомная аномалия (ХА) с частотой 1/10000-1/30000 живорожденных. В статье представлены клинические и молекулярно-цитогенетические характеристики двух неродственных пациентов с синдромом inv dup del(8p) и уточнены механизмы формирования хромосомного дисбаланса. Inverted duplication deletion 8p syndrome (inv dup del(8p), ORPHA 96092) is a rare chromosomal abnormality with a frequency of 1:10,000 - 30,000 newborns. Clinical manifestations of this syndrome include mental retardation, facial anomalies, hypoplasia/agenesis of corpus callosum, scoliosis and/or kyphosis, hypotonia, congenital heart defects. The article presents the clinical and molecular cytogenetic characteristics of two patients with inv dup del (8p) syndrome and clarifies the formation mechanisms.

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