Abstract
Background: The microorganisms are preferred over plants and animals for the bulk production of industrially important enzymes like pectinases because of their low production-cost, availability of fermentation raw material and the easily controllable production conditions. The pectinolytic potential of a large pool (130) of Bacillus cereus group and non-Bacillus cereus was evaluated. The effect of different physicochemical parameters on polygalacturonase(PGase) production by three promising strains, B. pumilusS76, B.thuringiensis S140(b) and B.taquilensis S140(c) with gene bank accession no. KU981112, KU981113 and KU981114, were studied and the enzyme was partially characterized. The enzyme activity was assayed using dinitrosalicylic acid method.Results: The polygalacturonases produced by Bp S76, Bt S140(b) and BtqS140(c) were thermostable at temperature above 55ºC with a melting temperature (Tm) of 72ºC,62ºC and 57ºC, respectively and the half-life (T1/2) at 60ºC of 50, 65 and 30 min., respectively. The catabolite repression was noted in presence of glucose while the production increased upon addition of 1% yeast extract to the growth medium. The enzyme activity was also induced in presence of Na+ and Ca+2.Conclusion: The strain Bp S76 appeared a better candidate for future biotechnological application owing to the production of higher titers (6.11 IU/L.hrs) of polygalacturonase with greater stability.
Lipase from New Isolate Bacillus cereus ATA179: Optimization of Production Conditions, Partial Purification, Characterization and Its Potential in the Detergent Industry
