The choice of auxin analogue for in vitro root induction influences post-induction root development in Eucalyptus grandis

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

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In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v20i1.5970 ◽

2010 ◽

Vol 20 (1) ◽

pp. 73-79 ◽

Cited By ~ 2

Author(s):

M. F. Hasan ◽

B. Sikdar

Keyword(s):

Plant Regeneration ◽

In Vitro Propagation ◽

Multiple Shoots ◽

Multiple Shoot ◽

Shoot Tips ◽

Root Induction ◽

Shoot Induction ◽

Multiple Shoot Induction ◽

Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words: Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

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The Influence of NAA, BA and Temperature on Shoot and Root Development from Begonia x cheimantha Petiole Segments Grown in vitro

Physiologia Plantarum ◽

10.1111/j.1399-3054.1974.tb03725.x ◽

1974 ◽

Vol 32 (1) ◽

pp. 49-54 ◽

Cited By ~ 15

Author(s):

MOGENS FONNESBECH

Keyword(s):

Root Development ◽

Begonia X Cheimantha

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The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

Notulae Scientia Biologicae ◽

10.15835/nsb335931 ◽

2011 ◽

Vol 3 (3) ◽

pp. 97-100

Author(s):

Naimeh SHARIFMOGHADAM ◽

Abbas SAFARNEJAD ◽

Sayed Mohammad TABATABAEI

Keyword(s):

Tissue Culture ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Base Material ◽

Culture Technique ◽

Induction Medium ◽

Root Induction ◽

Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

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Frequências de cortes em nove leguminosas forrageiras tropicais herbáceas cultivadas ao sol e sob plantação florestal

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-7119 ◽

2015 ◽

Vol 67 (3) ◽

pp. 809-818 ◽

Cited By ~ 1

Author(s):

M.L.F. Nicodemo ◽

F.H.D. Souza ◽

J.R.M. Pezzopane ◽

J.C.T. Mendes ◽

P. Tholon ◽

...

Keyword(s):

Eucalyptus Grandis

O objetivo deste trabalho foi estudar a produção e o valor nutritivo de nove espécies de leguminosas forrageiras herbáceas tropicais, cultivadas sob dois níveis de irradiação luminosa e seis frequências de corte na estação chuvosa, em Anhembi (SP), Brasil. Parcelas puras foram estabelecidas ao sol e sob plantação de Eucalyptus grandis, com 8 anos de idade, com 1.000 árvores/ha, que interceptou mais de 80% da radiação luminosa incidente. Avaliou-se a taxa de acúmulo de matéria seca, a proteína bruta e a digestibilidade in vitro da matéria seca em amostras de plantas colhidas em intervalos de 30, 60, 90 e 180 dias, com cortes adicionais aos 120 e 150 dias. Sob pleno sol, as leguminosas testadas toleraram cortes mais frequentes (intervalos de 30 - 90 dias), exceto o Lablab. Na sombra, o grau de tolerância variou conforme a espécie; em termos de produção de MS e de persistência, a Centrosema e a Puerária se destacaram dentre as demais leguminosas comparadas, quando os cortes foram realizados em intervalos de 60 dias, enquanto o Arachis e o Estilosantes foram os mais produtivos quando os cortes ocorreram em intervalos de 120 dias, mantendo o valor nutricional. O Alysicarpus e a Aeschynomene não persistiram sob o nível de sombreamento que prevaleceu nesse experimento.

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In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

Notulae Scientia Biologicae ◽

10.15835/nsb829817 ◽

2016 ◽

Vol 8 (2) ◽

pp. 161-163

Author(s):

Owk ANIEL KUMAR ◽

Songa RAMESH ◽

Sape SUBBA TATA

Keyword(s):

Large Scale ◽

Leaf Disc ◽

Basal Medium ◽

Shoot Proliferation ◽

Shoot Multiplication ◽

Medicinal Herb ◽

Root Induction ◽

Survival Percentage ◽

Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

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IBA AND MICROCUTTING COLLECTIONS IN THE MICROPROPAGATION OF Eucalyptus spp HYBRID CLONES.1

Revista Árvore ◽

10.1590/1806-90882017000600005 ◽

2017 ◽

Vol 41 (6) ◽

Author(s):

Ricardo Gallo ◽

Aloisio Xavier ◽

Luciana Coelho de Moura ◽

Brener de Almeida Oliveira ◽

Heloisa Rocha do Nascimento ◽

...

Keyword(s):

Eucalyptus Grandis ◽

Stem Diameter ◽

Residual Effects ◽

Seedling Height ◽

Seedling Vigor ◽

Eucalyptus Urophylla ◽

Ex Vitro ◽

Semisolid Medium ◽

Hybrid Clones

ABSTRACT This study aims to evaluate the effect of IBA concentrations and microcuttings successive collections in the micropropagation of Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones. Clumps containing six to eight buds of clones established in vitro were transferred to a 250 mL glass flask in JADS semisolid medium. Successive collections were performed every 20 days for Eucalyptus grandis x E. urophylla clone and every 30 days for Eucalyptus urophylla x E. globulus clone. The following variables were evaluated under in vitro conditions: number of shoots > 0.5 cm, number of microcuttings > 2 cm, length of the longest microcutting, and shoots vigor. Under ex vitro conditions, in the greenhouse and shade house, the following variables were evaluated: seedling height, percentage of survival, stem diameter, percentage of root observed at the lower end of the tube, and seedling vigor. In full sun (ex vitro), the following variables were analyzed: seedling height, stem diameter, survival, number of roots, root volume, seedling vigor, and shoot and root dry matter. Good in vitro microcuttings productivity was observed over the successive collections. IBA levels were adjusted for each clone, ranging from 0.25 to 0.50 mg L-1 for Eucalyptus grandis x E. urophylla clone, and from 0.75 to 1.0 mg L-1 for Eucalyptus urophylla x E. globulus clone. IBA concentrations led to residual effects under ex vitro conditions, providing good rooting and survival for Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones at IBA concentrations between 0.25 and 0.50 mg L-1 and between 0.50 and 1.0 mg L-1, respectively.

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ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

Revista Árvore ◽

10.1590/1806-90882017000100014 ◽

2017 ◽

Vol 41 (1) ◽

Author(s):

Leandro Silva Oliveira ◽

Aloisio Xavier ◽

Wagner Campos Otoni ◽

José Marcello Salabert Campos ◽

Lyderson Facio Viccini ◽

...

Keyword(s):

Flow Cytometry ◽

Microsatellite Markers ◽

Genetic Stability ◽

Culture Medium ◽

Eucalyptus Grandis ◽

Genetic Fidelity ◽

Shoot Multiplication ◽

Micropropagated Plants ◽

Hybrid Clones

ABSTRACT Flow cytometry and microsatellite markers were used to determine a genetic fidelity of micropropagated plants from the two Eucalyptus urophylla x E. globulus clones and a Eucalyptus grandis x E. globulus clone derived from adult material. Clones were repeatedly subcultured for 25 subcultures on MS medium supplemented with BA (2.22 µM) and ANA (0.05 µM) for in vitro shoot multiplication. The elongation was performed in MS culture medium supplemented with AIB (2.46 µM) and BA(0.22 µM). The ex vitro rooting and acclimatization phases were lead at the same time. The micropropagated clones showed genetic stability by flow cytometry and microsatellite markers. The results proved that micropropagation, for purposes of rejuvenation, can be a viable technique to generate genetically stable or identical E. globulus hybrid clones.

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