Production of Cold-active Extracellular α-Amylase by Newly Isolated Microbacterium foliorum GA2 from Gangotri glacier, Western Himalaya, India

2011 ◽  
Vol 3 (5) ◽  
pp. 449-459 ◽  
Author(s):  
Roohi . ◽  
M.Kuddus . ◽  
I.Z. Ahmad ◽  
J.M. Arif
Keyword(s):  
Western Himalaya ◽  
Cold Active ◽  
Gangotri Glacier

Psychrotrophic proteolytic bacteria from cold environment of Gangotri glacier, Western Himalaya, India

2005 ◽  
Vol 36 (5-6) ◽  
pp. 654-659 ◽  
Author(s):  
V.S. Baghel ◽  
R.D. Tripathi ◽  
P.W. Ramteke ◽  
K. Gopal ◽  
S. Dwivedi ◽  
...  
Keyword(s):  
Western Himalaya ◽  
Cold Environment ◽  
Proteolytic Bacteria ◽  
Gangotri Glacier

Cold-active extracellular alkaline protease from an alkaliphilicStenotrophomonas maltophilia: production of enzyme and its industrial applications

2009 ◽  
Vol 55 (11) ◽  
pp. 1294-1301 ◽  
Author(s):  
Mohammed Kuddus ◽  
Pramod W. Ramteke
Keyword(s):  
High Efficiency ◽  
Western Himalaya ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Maximum Activity ◽  
Exchange Chromatography ◽  
Protease Production ◽  
Cold Active ◽  
Metalloprotease Inhibitors ◽  
Potential Component

A novel psychro-tolerant bacterium Stenotrophomonas maltophilia (MTCC 7528) with an ability to produce extracellular, cold-active, alkaline, and detergent-stable protease was isolated from soil samples obtained from Gangotri glacier, Western Himalaya, India. The culture conditions for higher protease production were optimized with respect to incubation time, agitation, substrate, pH, and temperature. Maximum protease production of 56.2 U·mL–1was achieved in the medium at 20 °C and pH 9.0 after 120 h incubation. The protease was partially purified by ion-exchange chromatography and approximately 55-fold purification was achieved. The purified enzyme was a 75 kDa protease with maximum activity and stability at pH 10 and 20 °C. The activity of enzyme is stimulated by Mn2+and inhibited completely by metalloprotease inhibitors, indicating that it is a metalloprotease. The protease showed excellent stability and compatibility with commercial detergents and exhibited high efficiency for the removal of different types of protein-containing stains at low temperature. The wash performance analysis of blood and grass stains on cotton fabric showed an increase in reflectance by 26% and 23%, respectively, after treatment with enzyme in comparison to detergent only. These results indicate that it may be a potential component to use as a detergent additive for cold washing and in environmental bioremediation in cold regions.

Focus on Thermokarst Lakes in Indian Himalaya: Inception and Implication under Warming Climate

2020 ◽  
Vol 6 (2) ◽  
pp. 59-69
Author(s):  
Pratima Pandey ◽  
S. Nawaz Ali ◽  
Vikram Sharma ◽  
Prashant K. Champati Ray
Keyword(s):  
Western Himalaya ◽  
High Mountain ◽  
Thermokarst Lakes ◽  
Glacial Environments ◽  
Global Warming Scenario ◽  
Scientific Attention ◽  
Topographic Depressions ◽  
Permafrost Thawing ◽  
The Impact ◽  
Thaw Lakes

Thermokarst (Thaw) lakes are landforms found in topographic depressions created by thawing ground ice in permafrost zones. They play an important role in the regulation of climatic functions. These lakes are a manifestation of warming surface temperatures that accelerates the ice-rich permafrost to degrade by creating marshy hollows/ponds. In the current global warming scenario, the thermokarst lakes in the high mountain regions (Himalaya) are expected to grow further. This accelerate permafrost thawing which will affect the carbon cycle, hydrology and local ecosystems. This phenomenon has attracted huge scientific attention because it has led to a rapid mass change of glaciers in the region, including extensive changes occurring on peri-glacial environments. The most striking fact is the release of an enormous amount of greenhouse gases, including methane, carbon dioxide and nitrous oxide that is locked in these lakes. The present study delves into the thermokarst lakes in the upper reaches of Chandra Valley and Western Himalaya. The study also aims at designating the impact of their changes on the ecosystem, particularly their influence on the atmospheric greenhouse gas concentrations.

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