Cryoturbation leads to iron-organic carbon associations along a permafrost soil chronosequence in northern Alaska

In permafrost soils, substantial amounts of organic carbon (OC) are potentially protected from microbial degradation and transformation into greenhouse gases by association with reactive iron (Fe) minerals. As permafrost environments respond to climate change, increased drainage of thaw lakes in permafrost regions is predicted. Soils will subsequently develop on these drained thaw lakes, but the role of Fe-OC associations in future OC stabilization during this predicted soil development is unknown. To fill this knowledge gap, we have examined Fe-OC associations in organic, cryoturbated and mineral horizons along a 5500-year chronosequence of drained thaw lake basins in Utqiaġvik, Alaska. By applying chemical extractions, we found that ~17 % of the total OC content in cryoturbated horizons is associated with reactive Fe minerals, compared to ~10 % in organic or mineral horizons. As soil development advances, the total stocks of Fe-associated OC more than double within the first 50 years after thaw lake drainage, because of increased storage of Fe-associated OC in cryoturbated horizons (from 8 to 75 % of the total Fe-associated OC stock). Spatially-resolved nanoscale secondary ion mass spectrometry showed that OC is primarily associated with Fe(III) (oxyhydr)oxides which were identified by 57Fe Mössbauer spectroscopy as ferrihydrite. High OC:Fe mass ratios (>0.22) indicate that Fe-OC associations are formed via co-precipitation, chelation and aggregation. These results demonstrate that, given the proposed enhanced drainage of thaw lakes under climate change, OC is increasingly incorporated and stabilized by the association with reactive Fe minerals as a result of soil formation and increased cryoturbation.

Seasonal Regime Shift in the Viral Communities of a Permafrost Thaw Lake

Permafrost thaw lakes including thermokarst lakes and ponds are ubiquitous features of Subarctic and Arctic landscapes and are hotspots of microbial activity. Input of terrestrial organic matter into the planktonic microbial loop of these lakes may greatly amplify global greenhouse gas emissions. This microbial loop, dominated in the summer by aerobic microorganisms including phototrophs, is radically different in the winter, when metabolic processes shift to the anaerobic degradation of organic matter. Little is known about the viruses that infect these microbes, despite evidence that viruses can control microbial populations and influence biogeochemical cycling in other systems. Here, we present the results of a metagenomics-based study of viruses in the larger than 0.22 µm fraction across two seasons (summer and winter) in a permafrost thaw lake in Subarctic Canada. We uncovered 351 viral populations (vOTUs) in the surface waters of this lake, with diversity significantly greater during the summer. We also identified and characterized several phage genomes and prophages, which were mostly present in the summer. Finally, we compared the viral community of this waterbody to other habitats and found unexpected similarities with distant bog lakes in North America.

Focus on Thermokarst Lakes in Indian Himalaya: Inception and Implication under Warming Climate

Thermokarst (Thaw) lakes are landforms found in topographic depressions created by thawing ground ice in permafrost zones. They play an important role in the regulation of climatic functions. These lakes are a manifestation of warming surface temperatures that accelerates the ice-rich permafrost to degrade by creating marshy hollows/ponds. In the current global warming scenario, the thermokarst lakes in the high mountain regions (Himalaya) are expected to grow further. This accelerate permafrost thawing which will affect the carbon cycle, hydrology and local ecosystems. This phenomenon has attracted huge scientific attention because it has led to a rapid mass change of glaciers in the region, including extensive changes occurring on peri-glacial environments. The most striking fact is the release of an enormous amount of greenhouse gases, including methane, carbon dioxide and nitrous oxide that is locked in these lakes. The present study delves into the thermokarst lakes in the upper reaches of Chandra Valley and Western Himalaya. The study also aims at designating the impact of their changes on the ecosystem, particularly their influence on the atmospheric greenhouse gas concentrations.

Novel Psychrophiles and Exopolymers from Permafrost Thaw Lake Sediments

Thermokarst lakes are one of the most abundant types of microbial ecosystems in the circumpolar North. These shallow basins are formed by the thawing and collapse of ice-rich permafrost, with subsequent filling by snow and ice melt. Until now, permafrost thaw lakes have received little attention for isolation of microorganisms by culture-based analysis. The discovery of novel psychrophiles and their biomolecules makes these extreme environments suitable sources for the isolation of new strains, including for potential biotechnological applications. In this study, samples of bottom sediments were collected from three permafrost thaw lakes in subarctic Québec, Canada. Their diverse microbial communities were characterized by 16S rRNA gene amplicon analysis, and subsamples were cultured for the isolation of bacterial strains. Phenotypic and genetic characterization of the isolates revealed affinities to the genera Pseudomonas, Paenibacillus, Acinetobacter,Staphylococcus and Sphingomonas. The isolates were then evaluated for their production of extracellular enzymes and exopolymers. Enzymes of potential biotechnological interest included α and β-glucosidase, α and β-maltosidase, β-xylosidase and cellobiohydrolase. One isolate, Pseudomonas extremaustralis strain 2ASCA, also showed the capability to produce, in the loosely bound cell fraction, a levan-type polysaccharide with a yield of 613 mg/L of culture, suggesting its suitability as a candidate for eco-sustainable alternatives to commercial polymers.

Biogeochemistry of macrophytes, sediments and porewaters in thermokarst lakes of western Siberia in the discontinuous and continuous permafrost zone

<p>Despite high importance of macrophytes in shallow thaw lakes for control of major and trace nutrients in lake water, the chemical composition of different aquatic plants and trace element (TE) partitioning between macrophytes and lake water and sediments in the permafrost regions remain totally unknown. Here we sampled dominant macrophytes of thermokarst (thaw) lakes of discontinuous and continuous permafrost zones in Western Siberia Lowland (WSL) and we measured major and trace elements in plant biomass, lake water, lake sediments and sediment porewater. All 6 studies plants (Hippuris vulgaris L., Glyceria maxima (Hartm.) Holmb., Comarum palustre L., Ranunculus spitzbergensis Hadac, Carex aquatilis Wahlenb s. str., Menyanthes trifoliata L.), sizably accumulate macronutrients (Na, Mg, Ca), micronutrients (B, Mo, Nu, Cu, Zn, Co) and toxicants (As, Cd) relative to lake sediments. The accumulation of other trace elements including rare earth elements (REE) in macrophytes relative to pore waters and sediments was strongly species-specific. Under climate warmings scenario and the propagation of southern species northward, the accumulation of trace metals in aquatic plants of thermokarst lakes will produce preferential uptake of Cd, Pb, Ba from thermokarst lake water and sediments by the biomass of aquatic macrophytes. This may eventually diminish the transport of metal micronutrients from lakes to rivers and further to the Arctic Ocean.</p><p>Support from the RSF (RNF) grant 19-77-00073 “Experimental modeling of the formation mechanisms for elemental composition of water in thermokarst lakes of Western Siberia: vegetation effect”.</p>

Oxygen depletion in subarctic peatland thaw lakes

Permafrost thawing and erosion results in the enrichment of northern lakes by soil organic matter. These allochthonous inputs favour bacterial decomposition and may cause the draw-down of dissolved oxygen to anoxic conditions that promote methanogenesis. Our objective in the present study was to determine the seasonal variations in dissolved oxygen in a set of permafrost peatland lakes in subarctic Quebec, Canada, and to relate these changes to metabolic rates, ice cover, and mixing. The lakes had high dissolved organic carbon concentrations, and their surface waters in summer had greenhouse gas concentrations that were up to one (CO2) to three (CH4) orders of magnitude above air-equilibrium values, indicating their strongly heterotrophic character. Consistent with these observations, the peatland lakes had elevated rates of bacterial production and oxygen consumption. Continuous measurements of oxygen by in situ sensors and of ice cover by automated field cameras showed that the lakes became fully anoxic shortly after freeze-up. The waters were partially re-oxygenated by mixing events in spring and fall, but in one lake, the bottom waters remained anoxic throughout the year. These observations provide a foundation for subsequent biogeochemical and modelling studies of peatland thaw lakes as an abundant class of Arctic freshwater ecosystems.