scholarly journals Optimization of DNA Isolation and PCR Protocol for ISSR Analysis of Nothapodytes nimmoniana: A Threatened Anti-cancerous Medicinal Plant

2012 ◽  
Vol 4 (2) ◽  
pp. 100-107 ◽  
Author(s):  
Anuradha Sane ◽  
V.K. Abdul Kare ◽  
P.E. Rajasekhar ◽  
T. Vasantha K
Keyword(s):  
Medicinal Plant ◽  
Dna Isolation ◽  
Nothapodytes Nimmoniana ◽  
Issr Analysis

Optimization of DNA isolation and PCR protocol for analysis and evaluation of genetic diversity of the medicinal plant,Anemopsis californicausing RAPD

2013 ◽  
Vol 64 (2) ◽  
pp. 184-195 ◽  
Author(s):  
C. Lizette Del-Toro-Sánchez ◽  
S. Villaseñor-Alvarado ◽  
Florentina Zurita-Martínez ◽  
O. Castellanos-Hernández ◽  
Araceli Rodríguez-Sahagún ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
Dna Isolation ◽  
Analysis And Evaluation

ISSR analysis reveals high intraspecific variation in Rauvolfia serpentina L. – A high value medicinal plant

2012 ◽  
Vol 40 ◽  
pp. 192-197 ◽  
Author(s):  
Padmesh Pandaram Pillai ◽  
Jayakumari Sanakan Sajan ◽  
Kuttapetty Manikantan Menon ◽  
Kuttanappilly Sudhakara Panicker Jayakumar ◽  
Apian Subramoniam
Keyword(s):  
Medicinal Plant ◽  
Intraspecific Variation ◽  
Rauvolfia Serpentina ◽  
Issr Analysis

scholarly journals Review on Camptothecin Producing Medicinal Plant: Nothapodytes Nimmoniana

2021 ◽  
Vol 14 (4) ◽  
pp. 1799-1813
Author(s):  
Vanshika Shrivastava ◽  
Naveen Sharma ◽  
Vikas Shrivastava ◽  
Ajay Sharma
Keyword(s):  
Medicinal Plant ◽  
Extraction Methods ◽  
Good Source ◽  
Nothapodytes Nimmoniana ◽  
Endangered Medicinal Plant ◽  
Analytical Protocol ◽  
Western Ghats Of India ◽  
Global Demand ◽  
Pharmacological Data ◽  
The Western Ghats

Nothapodytes nimmoniana is an endangered medicinal plant widely distributed throughout the Western Ghats of India. The plant contains camptothecin (CPT) which is renowned anticancer drugs. Though, CPT found in many plant species but maximum amount of CPT has been reported from N. nimmoniana. Due to very good source of CPT, this plant has been explored for its Phytochemical, Biotechnological and Pharmacological aspects. Looking to the huge global demand for CPT, overexploitation of N. nimmoniana, unplanned deforestation, and lowest production of CPT from intact plant, reduction of seed germination, high market cost and not have economically feasible process of production has optimistic us to investigate this plant in a systematic manner. The proposed article can be utilized for the establishment of extraction methods and analytical protocol for CPT. Also, Bioreactors production of CPT using high yielding cell line of N. nimmoniana. The pharmacological data will be applicable for discovery of new Drug and development lead to novel compounds which are safe and effective.

scholarly journals PCR ASSAY BASED MOLECULAR CHARACTERIZATION OF JUSTICIA BETONICA LINN., THOTTEA SILIQUOSA LAM., MEMECYLON EDULE ROXB., PAJANELIA LONGIFOLIA (WILLD) K. SCHUM AND BAUHINIA ACCUMINATA LINN

2020 ◽  
Vol 11 (6) ◽  
pp. 149-156
Author(s):  
A Shahul Hameed ◽  
Jollykutty Eapen ◽  
Ansary P Y ◽  
Indulekha V C ◽  
Resny AR
Keyword(s):  
Medicinal Plants ◽  
Medicinal Plant ◽  
Molecular Characterization ◽  
Plant Species ◽  
Dna Isolation ◽  
Medicinal Plant Species ◽  
Accurate Identification ◽  
Scientific Experiments ◽  
Ncbi Blast

Characterization of plants with DNA markers is an ideal approach for identification of medicinal plant species as they provide objectively analyzable data for accurate and fast identification. There are many medicinal plants described in ancient literatures that have not been exploited for their medicinal values. A documented treasure of traditional knowledge of Kerala’s Ayurvedic and folklore practices is the ‘Hortus Malabaricus’. Description of various useful medicinal plants and their use as single drug can be seen in this ethnomedicinal compendium. The plants Justicia betonica Linn., Thottea siliquosa Lam., Memecylon edule Roxb., Pajanelia longifolia (Willd) K. Schum.) and Bauhinia accuminata Linn described in Hortus Malabaricus are considered to possess immense therapeutic potentials. As the Ayurveda industry as well as practitioners are facing severe scarcity of required medicinal plants, search for establishing the claimed pharmacologic values of hitherto unexplored plants is to be promoted. For this purpose, their proper identification and authentication is needed as a first step of scientific experiments. The molecular characterization including DNA isolation and sequencing provides fast and accurate identification of medicinal plant species. In this project, the molecular characterization of Justicia betonica, Thottea siliquosa, Memecylon edule, Pajanelia longifolia and Bauhinia accuminata, including DNA isolation and quantification, NCBI-BLAST analysis and DNA sequencing was done. The sequence analysis of Thottea siliquosa, Memecylon edule, Bauhinia accuminata and Justicia betonica by BLASTN (NCBI) is revealed 99 to 100 % nucleotide sequence homology with different database sequences enabling species level confirmation.

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