Synergetic Effect of Rhizosphere Bacteria Isolates and Composted Manure on Fusarium Wilt Disease of Tomato Plants

2016 ◽  
Vol 11 (1) ◽  
pp. 20-27 ◽  
Author(s):  
Anbessa Dabassa ◽  
Lata Lachisa
2020 ◽  
Author(s):  
Halima Z. Hussein ◽  
Shaker I. Al-Dulaimi

AbstractChemical approaches have been applied to combat Fusarium wilt disease for a long time. Even though pesticides are effective in controlling the disease, they continue to damage the environment. Environmental-friendly approaches to manage plant disease are the goal of many studies recently. This study was conducted to assess the efficacy of some bio-agents in induction of systemic resistance in tomato plants as a management approach of Fusarium wilt disease caused by Fusarium oxysporum f.sp. lycopersici (FOL) under condition Plastic house. Results of the plastic house experiments showed that all treatments in decreased Fusarium disease percentage and severity on tomato, two bacterial combinations (Streptomyces sp. (St) and Pseudomonas fluorescence (Pf)) decreased the infection percentage and disease severity with 16.6% and 8.3%, respectively. Treatment with St reduced the infection percentage and disease severity with 33.3% and 22.8%, while the Pf treatment showed 41.6% and 31.2% reduction in infection percentage and disease severity, compared to 100% and 91.6% in the control treatment. Results of induced systemic resistance (ISR) biochemical indicators showed significant differences in tomato plants. Peroxidase and Phenylalanine-Ammonia-Lyase (PAL) activity and the Phenol content increased significantly 14 days after treatments compared to the control treatment, which contains only the fungal pathogen FOL.


2020 ◽  
Vol 3 (2) ◽  
pp. 20-30
Author(s):  
Muhammad Fazil ◽  
Tjut Chamzurni ◽  
Rina Sriwati

Abstrak: Fusarium oxysporum f.sp. lycopersici (Fol) adalah salah satu patogen tular tanah yang sangat berbahaya bagi tanaman tomat, hal itu dikarenakan patogen ini mampu bertahan dalam jangka waktu yang lama di dalam tanah. Salah  satu  alternatif  pengendalian  secara  biologi  yang  ramah  lingkungan  adalah dengan cara memanfaatkan cendawan antagonis sebagai agen biokontrol yaitu Trichoderma spp. penggunaan cendawan antagonis sebagai agen hayati harus dalam bentuk formula yang tepat dengan bahan yang mudah tersedia. Penelitian  ini   bertujuan  untuk  mengetahui  pengaruh  aplikasi  beberapa   bentuk formulasi  Trichoderma  spp  dalam  mengendalikan penyakit  layu  fusarium  pada  tanaman tomat. Penelitian ini menggunakan Rancangan Acak Lengkap pola non faktorial yang terdiri dari 4 perlakuan dengan menggunakan 5 ulangan, setiap ulangan terdiri dari 4 unit tanaman. Susunan perlakuan bentuk formulasi berbahan aktif Trichoderma spp yaitu F0 = Kontrol (tanpa perlakuan),F1 = Formulasi pelet daun katuk 3 g (+ 10 butir)/polibag, F2 = Formulasi padat jagung kering 3 g/polibag dan F3 = Formulasi cair produk komersil 100 ml/polibag. Hasil penelitian menunjukkan bahwa aplikasi beberapa bentuk formulasi  Trichoderma spp  mampu memberikan pengaruh yang nyata terhadap persentase layu tanaman dan tinggi tanaman serta persentase batang yang xylemnya terdiskolorasi. Application of Several Forms by Trichoderma spp Formulation in Controlling Fusarium Wilt Disease on Tomato PlantsAbstract. Fusarium oxysporum f.sp. lycopersici (Fol) is one of the soil pathogens that is very harmful for tomato plants, it is because these pathogens are able to survive for a long time in the soil. One of the alternative biological controls that are environmentally friendly is by utilizing the antagonist fungus as a biocontrol agent that is Trichoderma spp. the use of antagonistic fungi as biological agents should be in the form of the right formula with easily available materials. This study aims to determine the effect of application of some form of Trichoderma spp formulation in controlling fusarium wilt disease in tomato plants. This research uses Completely Randomized Design of non factorial pattern consisting of 4 treatments using 5 replications, each replication consisting of 4 plant units. the active formulation by several form formulation of Trichoderma spp is F0 = Control (without treatment), F1 = Leaf pelet formulation 3 g (+ 10 grain) / polybag, F2 = Dry corn solid formula 3 g / polybag and F3 = Commercial liquid product formulation 100 ml / polybag. The results showed that the application of some form of formulation Trichoderma spp able to give a real effect on the percentage of wilting plants and plant height as well as the percentage of stems that discoloration on xylem.


2018 ◽  
Vol 15 (1) ◽  
pp. 141-149
Author(s):  
Nguyễn Thanh Vũ ◽  
Huỳnh Quyền ◽  
Dương Hoa Xô ◽  
Lê Quang Luân

Chitosan with molecular weight (Mw) approx. 573,2 kDa and deacetylation degree about 80% was irradiated by gamma rays at swollen state in the present of 1 - 3% hydrogen peroxide for degradation to prepare low Mw products. The solution of 5% low Mw chitosan in 0.5% acetic acid was further irradiated for induction of oligochitosan. The obtained results indicated that the foliar application by 100 ppm oligochitosan with Mw ~ 19.5 kDa prepared by 4kGy-irradiation of the low Mw chitosan with Mw ~ 48.4 kDa had a strongly growth promotion effect on tomato plants after 30 days cultivation. The oligochitosan silver nanoparticles (AgNPs) was also synthesized by gamma irradiation of solution contained 5% low Mw chitosan and 1 mM silver nitrate in order to prepared antifungal fertilizer for tomato plants. The results on in vitro test for the antifungal activity oligochitosan AgNPs against Fusarium oxysporum causing Fusarium wilt disease pointed out that the addition of this product into cultivated media inhibited strongly the growth of the Fusarium oxysporum pathogen fungus on carrot agar media. The antifungal efficiency values were found from 50.5 to 94.9% by the supplementions of silver concentrations from 20 to 30 ppm, respectively. The supplementation with a silver concentration of 40 ppm inhibited completely the growth of F. oxysporum on the cultivated media. Thus the oligochitosan AgNPs product had not only the growth promotion activity for tomato, but also high antifungal activity against F. oxysporum. This product is promising to be used as an antifungal fertilizer for tomato with safety and efficiency.


2017 ◽  
Vol 35 (1) ◽  
pp. 027-035
Author(s):  
Alaa Ibrahim ◽  
◽  
Omar Hmmoudi ◽  
George Asmar ◽  
Naser Sheikh Suleiman ◽  
...  

Author(s):  
Ahmed M. Aldinary ◽  
Amer Morsy Abdelaziz ◽  
Ayman A. Farrag ◽  
Mohamed S. Attia

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