In vitro Evaluation of Trichoderma Species Against Fusarium oxysporum f. sp. lycopersici Causing Tomato Wilt

The potential of biocontrol agentsPurpureocillium lilacinum(Paecilomyceslilacinus) andTrichodermaharzianumwas evaluated against tomato wilt complex, caused by a combination ofMeloidogyne incognitaandFusarium oxysporumf. sp.lycopersici, under both laboratory and field conditions. Biocontrol agents at spore concentration of 1 × 106spores ml−1were applied alone and in combined treatments. The results of combined application revealed maximum mortality and inhibition of hatching ofM. incognitaunderin vitroconditions. Combined application of both antagonistic fungi was found to be more effective in mycelial inhibition ofFusarium oxysporumf. sp.lycopersici. In glasshouse trials, application ofT. harzianumpromoted overall plant growth, followed by combined application ofP. lilacinumandT. harzianum; nematode development parameters and fungus damage were significantly reduced. Under field conditions, the combined application ofP. lilacinumandT. harzianumincreased the number of leaves, shoot length, shoot weight and root length, and decreased root weight, with minimum number of females and egg masses ofM. incognitaper root system and mycelia inhibition ofF. oxysporum.

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In vitro, evaluation of some medicinal plants extracts against Fusarium oxysporum f. sp. lycopersici

Scientific Journal for Damietta Faculty of Science ◽

10.21608/sjdfs.2016.194523 ◽

2016 ◽

Vol 6 (1) ◽

pp. 20-27

Author(s):

Zakaria Baka ◽

Mahmoud Nour El-Din ◽

M Abodobara ◽

Fatma Elzahra El-Menyer

Keyword(s):

Medicinal Plants ◽

Fusarium Oxysporum ◽

In Vitro Evaluation

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Pengembangan Teknologi Pengendalian Penyakit Layu Tomat (Fusarium oxysporum f. sp. lycopersici) yang Ramah Lingkungan dengan Menggunakan Mikroorganisme Saprofit

Agrotek ◽

10.30862/agt.v2i1.431 ◽

2018 ◽

Vol 2 (1) ◽

Author(s):

Derek Kornelis Erari ◽

Eko Agus Martanto

Keyword(s):

Biological Control ◽

Fusarium Oxysporum ◽

Growth And Development ◽

Wilt Disease ◽

Common Disease ◽

Microbial Antagonism ◽

The Earth ◽

Tomato Wilt

Fusarium wilt disease is a common disease for tomatoes plant which damages plant although it is intensively cultivated. Biological control by microbial antagonism is environmentally friendly compare to the use of fungicide. This research was aimed to study the capability of Bacillus subtilis, Pseudomonas fluorescens, Gliocladium fimbriatum and Trichoderma viridae that are locally specific in controll Fusarium oxysporum f.sp. lycopersici� cause tomato wilt disease. Evaluation of F.o. f.sp. lycopersici� antagonism capability was done by in vitro and in vivo.� In vitro antagonism treatment used the ouble plating treatment on Potato Dextose Agar.� In vivo antagonism treatment was done by gave antagonism agent and F.o. f.sp. lycopersici� together to the earth medium. The result of the study revealed that (1) B. subtilis, G. fimbriatum and T. viridae can pressure the growth and development �F.o. f.sp. lycopersici which cause tomato wilt disease both in vitro and in vivo, (2) In vitro the average of the prevented pathogen growth was as follow: B. subtilis (55.52%), G. fimbriatum (36.77%) and� T. viridae (35.87%), (3) In vivo treatment by the earth medium was not significantly different, and (4) In vivo the ability of prevent disease intensity was as follow : B. subtilis (62.35%), G. fimbriatum (49.95%) and� T. viridae (49.95%).

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