Antibody response to recombinant HCV antigens and detection of HCV RNA in serum samples of blood donors positive for anti-HCV by 2nd generation HCV antibody screening test (supplement). A comparison of reaction between PHA & PAII.

A novel DNA virus designated TT virus (TTV) has been reported to be involved in the development of posttransfusion non–A-C hepatitis. We evaluated the frequency and natural course of TTV infection in a cohort of transfusion-dependent thalassemic patients in a 3-year follow-up study. Ninety-three serum hepatitis C virus (HCV) antibody-negative patients (median age of 8 years; range, 0 to 25) from eight centers were studied. Of them, 34 (37%) had an abnormal alanine-aminotransferase (ALT) baseline pattern, and the other 12 (13%) showed ALT flare-ups during the follow-up. TTV DNA in patient sera collected at the time of enrollment and at the end of follow-up was determined by polymerase chain reaction (PCR). In parallel, serum samples from 100 healthy blood donors were also tested. At baseline, 87 patient sera (93.5%) tested positive for the TTV DNA. Of these TTV DNA-positive patients, 84 (96.5%) remained viremic at the end of the study period. Of the 6 TTV DNA-negative patients, 3 acquired TTV infection during follow-up. However, no definite relation was observed between the results of TTV DNA determination and ALT patterns. TTV viremia was also detectable in 22% of blood donors. In conclusion, TTV infection is frequent and persistent among Italian transfusion-dependent patients. The high rate of viremia observed in healthy donors indicates that the parenteral route is not the only mode of TTV spread.

Download Full-text

Prediction of hepatitis C virus infectivity in seropositive Australian blood donors by supplemental immunoassays and detection of viral RNA

Blood ◽

10.1182/blood.v78.9.2462.bloodjournal7892462 ◽

1991 ◽

Vol 78 (9) ◽

pp. 2462-2468

Author(s):

JP Allain ◽

PJ Coghlan ◽

KG Kenrick ◽

K Whitson ◽

A Keller ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Blood Donors ◽

Hcv Rna ◽

Virus Infectivity ◽

Gene Products ◽

Serum Samples ◽

Immunoblot Assay ◽

Positive Serum ◽

Polymerase Chain

The prevalence of anti-hepatitis C virus (HCV) enzyme immunoassay (EIA)- positive in 167,511 Australian volunteer blood donors from Adelaide, Melbourne, Perth, and Sydney was 0.78%. One thousand two-hundred and eighteen EIA-positive serum samples were assessed by supplemental tests including a blocking EIA and two peptide EIAs corresponding to major epitopes of the HCV C-100–3 antigen. Seven hundred and eighteen samples (59%) were negative by supplemental testing; no evidence of reactivity with other HCV gene products or HCV RNA detected by cDNA polymerase chain reaction was found in selected samples from this group. In contrast, of 43 samples randomly selected from 400 samples (32.8%) positive by supplemental testing, 88% were reactive with HCV 33-C or core antigens and 52% contained HCV RNA, suggesting contact with HCV and infectivity of most donors in this group. Most samples equivocal by supplemental testing reacted only with C-100 and not with other HCV antigens when tested by dot immunoblot assay. Only 21% had detectable HCV RNA. The battery of assays used in this study indicated that approximately 32% of HCV EIA repeatably reactive serum samples were serologically related to HCV, corresponding to a 0.25% prevalence of potentially infectious donors.

Download Full-text

Multicenter evaluation of a fully automated third-generation anti-HCV antibody screening test with excellent sensitivity and specificity

Medical Microbiology and Immunology ◽

10.1007/s00430-010-0171-0 ◽

2010 ◽

Vol 200 (2) ◽

pp. 77-83 ◽

Cited By ~ 35

Author(s):

F. Alborino ◽

A. Burighel ◽

F.-W. Tiller ◽

J. van Helden ◽

C. Gabriel ◽

...

Keyword(s):

Sensitivity And Specificity ◽

Screening Test ◽

Third Generation ◽

Antibody Screening ◽

Hcv Antibody

Download Full-text

A Prospective Study on TT Virus Infection in Transfusion-Dependent Patients With β-Thalassemia

Blood ◽

10.1182/blood.v93.5.1502 ◽

1999 ◽

Vol 93 (5) ◽

pp. 1502-1505 ◽

Cited By ~ 52

Author(s):

Daniele Prati ◽

Yu-Huei Lin ◽

Claudia De Mattei ◽

Jen-Kuei Liu ◽

Elena Farma ◽

...

Keyword(s):

Blood Donors ◽

High Rate ◽

Chain Reaction ◽

Serum Samples ◽

Tt Virus ◽

Healthy Donors ◽

Hcv Antibody ◽

Polymerase Chain ◽

A Prospective Study

Abstract A novel DNA virus designated TT virus (TTV) has been reported to be involved in the development of posttransfusion non–A-C hepatitis. We evaluated the frequency and natural course of TTV infection in a cohort of transfusion-dependent thalassemic patients in a 3-year follow-up study. Ninety-three serum hepatitis C virus (HCV) antibody-negative patients (median age of 8 years; range, 0 to 25) from eight centers were studied. Of them, 34 (37%) had an abnormal alanine-aminotransferase (ALT) baseline pattern, and the other 12 (13%) showed ALT flare-ups during the follow-up. TTV DNA in patient sera collected at the time of enrollment and at the end of follow-up was determined by polymerase chain reaction (PCR). In parallel, serum samples from 100 healthy blood donors were also tested. At baseline, 87 patient sera (93.5%) tested positive for the TTV DNA. Of these TTV DNA-positive patients, 84 (96.5%) remained viremic at the end of the study period. Of the 6 TTV DNA-negative patients, 3 acquired TTV infection during follow-up. However, no definite relation was observed between the results of TTV DNA determination and ALT patterns. TTV viremia was also detectable in 22% of blood donors. In conclusion, TTV infection is frequent and persistent among Italian transfusion-dependent patients. The high rate of viremia observed in healthy donors indicates that the parenteral route is not the only mode of TTV spread.

Download Full-text

A study on the HCV antibody screening test in donor blood for prevention of posttransfusional non-A non-B hepatitis in Tokushima Prefecture.

Journal of the Japan Society of Blood Transfusion ◽

10.3925/jjtc1958.37.787 ◽

1991 ◽

Vol 37 (6) ◽

pp. 787-794

Author(s):

Yoshifumi Kajimoto ◽

Tsukasa Takaoka ◽

Susumu Ito

Keyword(s):

Screening Test ◽

Donor Blood ◽

Antibody Screening ◽

Hcv Antibody

Download Full-text

Prediction of hepatitis C virus infectivity in seropositive Australian blood donors by supplemental immunoassays and detection of viral RNA

Blood ◽

10.1182/blood.v78.9.2462.2462 ◽

1991 ◽

Vol 78 (9) ◽

pp. 2462-2468 ◽

Cited By ~ 22

Author(s):

JP Allain ◽

PJ Coghlan ◽

KG Kenrick ◽

K Whitson ◽

A Keller ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Blood Donors ◽

Hcv Rna ◽

Virus Infectivity ◽

Gene Products ◽

Serum Samples ◽

Immunoblot Assay ◽

Positive Serum ◽

Polymerase Chain

Abstract The prevalence of anti-hepatitis C virus (HCV) enzyme immunoassay (EIA)- positive in 167,511 Australian volunteer blood donors from Adelaide, Melbourne, Perth, and Sydney was 0.78%. One thousand two-hundred and eighteen EIA-positive serum samples were assessed by supplemental tests including a blocking EIA and two peptide EIAs corresponding to major epitopes of the HCV C-100–3 antigen. Seven hundred and eighteen samples (59%) were negative by supplemental testing; no evidence of reactivity with other HCV gene products or HCV RNA detected by cDNA polymerase chain reaction was found in selected samples from this group. In contrast, of 43 samples randomly selected from 400 samples (32.8%) positive by supplemental testing, 88% were reactive with HCV 33-C or core antigens and 52% contained HCV RNA, suggesting contact with HCV and infectivity of most donors in this group. Most samples equivocal by supplemental testing reacted only with C-100 and not with other HCV antigens when tested by dot immunoblot assay. Only 21% had detectable HCV RNA. The battery of assays used in this study indicated that approximately 32% of HCV EIA repeatably reactive serum samples were serologically related to HCV, corresponding to a 0.25% prevalence of potentially infectious donors.

Download Full-text