scholarly journals Effect of agro-industrial residues mixtures on the production of endoglucanase by Aspergillus niger in solid state fermentation

2019 ◽  
Vol 42 ◽  
pp. e41358
Author(s):  
Fernanda de Oliveira ◽  
Marcelo Rodrigues de Melo ◽  
João Batista Buzato
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Rice Straw ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Industrial Waste ◽  
Cellulose Degradation ◽  
Maximum Activity ◽  
Soybean Hulls ◽  
Industrial Residues

The low-cost production of cellulolytic complexes that present high action at mild conditions is one of the major bottlenecks for the economic viability of the production of cellulosic ethanol. The influence of agro-industrial residues was assessed to enhance endoglucanase production by Aspergillus niger 426 grown in solid state fermentation. The highest percentage of lignin degradation was found on soybean hulls (56%) followed by sugarcane bagasse (36%) and rice straw (8.5%). The cellulose degradation, around 90%, was observed on soybean hulls and sugarcane bagasse, but only 50% on rice straw, and maximum production of endoglucanase (112.34 ± 0.984 U mL-1) was observed for soybean hulls. The best Experimental Mixture Design condition was under cultivation of 2.5 g of sugarcane bagasse, 2.3 g of rice straw and 5.2 g of soybean hulls, leading to a maximum activity of 138.92 ± 0.02 U mL-1. The statistical methodology enabled an increase of over 20% in the production of endoglucanase using agro-industrial waste. These data demonstrate that A. niger 426 is a potential source of cellulases which can be obtained by solid state fermentation using agro-industrial waste.

Construction of the Starter to Produce Lignocellulases via Solid State Fermentation of Rice Straw

2011 ◽  
Vol 356-360 ◽  
pp. 1819-1822
Author(s):  
Shan Shan Du ◽  
Lu Yang ◽  
Jian Zhang Lu ◽  
Qing Qing Li ◽  
Juan Xu ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Rice Straw ◽  
Solid State Fermentation ◽  
Low Cost ◽  
Natural Microflora ◽  
Special Strain

The study demonstrates the starter construction and its solid state fermentation (SSF) using rice straw as substrate for lignocellulases production. The starter is constructed by natural microflora growing on rice straw additionally reinforced with Aspergillus niger ZJU-RYD1, using the SSF substrate rice straw with 90% moisture content, successfully produced the integrate and high active lignocellulases at about 72 h, i.e. CMCase 12.44 U/g, cellobiase 12.05 U/g, FPAase 3.18 U/g, xylanase 365.61 U/g, lassase 2.48 U/g. In this study the strategy is satisfactorily developed to construct the starter for high effective and low-cost production of lignocellulases using rice straw as substrate, based on natural microflora and additional reinforcing with special strain.

scholarly journals Preparation of Aspergillus niger 426 naringinases for debittering citrus juice using of agro-industrial residues

2021 ◽  
Author(s):  
Fernanda de Oliveira ◽  
Tereza Cristina Luque Castellane ◽  
Marcelo Rodrigues de Melo ◽  
João Batista Buzato
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Grapefruit Juice ◽  
Maximum Activity ◽  
Industrial Residues ◽  
Juice Processing ◽  
Grapefruit Peel ◽  
Definition Of ◽  
Medium Supplementation ◽  
Sequencing Procedure

Abstract Naringin (4,5,7-trihydroxy flavanone-7-rhamnoglucoside), considered as the main bitter component of grapefruit, requires the use of enzymes to reduce the level of this substance during juice processing. For this reason, it has been the focus of many studies. To increase the production of naringinase by Aspergillus niger cultivated in solid-state fermentation (SSF), it was verified whether the influence of agro-industrial residues as fermentation substrates and, finally, selected the best of the three inducers, or their mixtures to remove the bitterness of grapefruit juice. Cultivation with 2.3 g of grapefruit peel, 2.5 g of rice bran, and 5.2 g of wheat bran and medium supplementation with a mixture of naringin, rutin, and hesperidin in the concentration of (g / L): 2, 5, 4.5, and 3.0, respectively, leading to a maximum activity of 28 U / mL. The results indicate that the sequencing procedure, which allowed the definition of an optimal mixture of components, is a new way for microorganisms to have a high naringinase yield, in particular by SSF, since our data showed a 96% increase in the production of naringinase.

Multi‐enzymatic recovery of fungal cellulases ( Aspergillus niger ) through solid‐state fermentation of sugarcane bagasse

2021 ◽  
Author(s):  
Tales Prado Alves ◽  
Carina Contini Triques ◽  
Edson Antonio da Silva ◽  
Marcia R. Fagundes‐Klen ◽  
Salah Din Mahmud Hasan
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation

scholarly journals Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste

2020 ◽  
Vol 6 ◽  
pp. 331-335 ◽  
Author(s):  
Dwini Normayulisa Putri ◽  
Andy Khootama ◽  
Meka Saima Perdani ◽  
Tania Surya Utami ◽  
Heri Hermansyah
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Industrial Waste ◽  
Lipase Production

scholarly journals Citric Acid Production by Aspergillus niger through Solid-State Fermentation on Sugarcane Bagasse

1970 ◽  
Vol 25 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Abdullah Al-Mahin ◽  
Shek Mahdi Hasan ◽  
Mahboob Hossain Khan ◽  
Rehana Begum
Keyword(s):  
Citric Acid ◽  
Solid State ◽  
Aspergillus Niger ◽  
Sugarcane Bagasse ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Sucrose Solution ◽  
Citric Acid Production ◽  
Sugar Utilization ◽  
Ig G

Four isolates of Aspergillus niger (viz., CA16, 14/20, HB3 and 318) were used for citric acid production using sugarcane bagasse as a substrate and sucrose solution as a moistening agent. Using 3 g of sugarcane bagasse as substrate, the highest citric acid production was obtained when 10 ml of 14% sucrose solution was used as moistening agent. Maximum citric acid production was found after 11 days fermentation for all isolates of A. niger. Both sugar utilization as well as citric acid production was found highest in the presence of Prescott salt by A. niger 318 (196.21 ìg/g), followed next by A. niger 14/20 (103.06 ìg/g). However, in absence of Prescott salt both sugar utilization and citric acid production was highest (50.01 ìg/g) by A. niger 14/20. In general, extension of the fermentation (up to 11 days) resulted in an increase in citric acid, total titratable acid and biomass, and decrease in both residual sugar concentrations. Keywords: Citric acid; Solid-state fermentation; Aspergillus niger; Sugarcane bagasseDOI: http://dx.doi.org/10.3329/bjm.v25i1.4664 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 9-12

scholarly journals Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes

2019 ◽  
Vol 4 (1) ◽  
pp. 105-111
Author(s):  
Kahar Muzakhar ◽  
Rudju Winarsa
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Maximum Activity ◽  
Reducing Sugar ◽  
Sugar Production ◽  
Coffee Pulp ◽  
Alkali Extract ◽  
Ph Range ◽  
Hydrolysis Activity

 An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C. 

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