Utilization of spent millet from locally fermented drink (Burukutu) as experimental diets for Clarias gariepinus

Brewer’s waste is one of the promising carbohydrate and protein source by-products for fish diets. A 10-wk feeding trial experiment involving 10 different diets (10 diets for Spent millet, from locally fermented drink (Burukutu) with increasing levels of brewer’s waste (40% crude protein) was carried out to evaluate the use of spent millet, from Burukutu production in Clarias gariepinus diets in place of normal Corn. Growth performance was compared against a control diet formulated to have similar composition to a typical commercial diet. 10 experimental diets replaced successively 0%, 10%, 20%, 30%, 40%, 50%, 70%, 80%, 90%, and 100% of the Corn carbohydrate with Spent millet from locally fermented drink, Burukutu.. The diets were isonitrogenous and isocaloric. A relatively good growth performance and nutrient utilization by Clarias gariepinus fed the test diets were observed. However, the results showed significant difference (p<0.05) in weight gain, SGR, FCR and RGR between treatments, with the control performing best. The result also showed that survival rate was high in all the treatment which indicate that, spent millet from burukutu production has no adverse effect on the experimented fish. Based on this research, it wasconcluded that 50% of the corn carbohydrate in a typicalcommercial diet could be replaced with spent grain (millet) from locally fermented drink (BKT) withno adverse effect on growth and feed utilization for Clarias gariepinus.

Sensory Features Introduced by Brewery Spent Grain with Impact on Consumers’ Motivations and Emotions for Fibre-Enriched Products

The aim of this work was both to formulate three different fibre-enriched products by the addition of Brewery Spent Grain (BSG), and to evaluate the impact of this fibre enrichment on sensory quality, acceptability, and purchase intention under blind conditions. BSG was incorporated into bread, pasta, and chocolate milk at levels of 8.3%, 2.8%, and 0.35% (w/w), respectively. The fibre-enriched products and their regular counterparts were evaluated together by consumers through a CATA questionnaire, the EsSense 25 Profile, an overall acceptability rating, and a purchase intention ranking. Although fibre-enriched bread and chocolate milk ranked lower in overall acceptability compared with their counterparts, no significant difference was found for fibre-enriched pasta (p > 0.05). Purchase intention did not differ significantly for both bread and pasta (p > 0.05), yet the reasons for purchasing them differed significantly (p < 0.05). Consumers recognised the fibre enrichment in these two products and, therefore, were willing to partially compromise on sensory attributes. The fibre-enriched chocolate milk, nonetheless, scored significantly (p < 0.05) lower in purchase intention than the control. This work demonstrates that the effect of BSG addition is product-specific, and that fibre perception makes consumers feel more confident.

Influence of the composition of nutrient media on the productivity and biological activity of the strain of entomopathogenic fungus Beauveria bassiana

The productivity and biological activity of the entomopathogenic fungus Beauveria bassiana (F-145) strain during the liquid-phase cultivation on various substrates for developing a biopesticide in its native form was analyzed (the research was carried out in 2019). For submerged cultivation, by-products from dairy and beer production (milk whey and brewing spent grain liquor) were used as components of the nutrient medium with addition of diesel fuel (DF) and Tween-80 as inducers of biological activity. It has been established, that the productivity of the strain on industrial by-product substrates was 1.5-2 times higher than on the Czapek medium. A high yield of a mycelial biomass with a titer of 108 -1010 CFU/ml was shown in a 5-day suspension based on a mixture of milk whey and brewing spent grain liquor. The biological activity of the culture suspension of the strain was determined. It was shown that the nematicidal activity of Beauveria bassiana strain with regard to nematodes of the Rhabditis sp. was largely manifested in a suspension obtained on a mixed medium with the addition of inducers. Ninety per cent death at mobile nematode stages was registered within one or two days of test-organism incubation. A complex nutrient medium composition containing by-products and inducers contributed to the preservation of the biological activity of the strain. The strain nematicidal activity was established at the level of 67-80 per cent with a titer of 106 -107 CFU/ml when the suspension was stored for 67 days.