Preparation of Aspergillus niger 426 naringinases for debittering citrus juice using of agro-industrial residues

Abstract Naringin (4,5,7-trihydroxy flavanone-7-rhamnoglucoside), considered as the main bitter component of grapefruit, requires the use of enzymes to reduce the level of this substance during juice processing. For this reason, it has been the focus of many studies. To increase the production of naringinase by Aspergillus niger cultivated in solid-state fermentation (SSF), it was verified whether the influence of agro-industrial residues as fermentation substrates and, finally, selected the best of the three inducers, or their mixtures to remove the bitterness of grapefruit juice. Cultivation with 2.3 g of grapefruit peel, 2.5 g of rice bran, and 5.2 g of wheat bran and medium supplementation with a mixture of naringin, rutin, and hesperidin in the concentration of (g / L): 2, 5, 4.5, and 3.0, respectively, leading to a maximum activity of 28 U / mL. The results indicate that the sequencing procedure, which allowed the definition of an optimal mixture of components, is a new way for microorganisms to have a high naringinase yield, in particular by SSF, since our data showed a 96% increase in the production of naringinase.

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Effect of agro-industrial residues mixtures on the production of endoglucanase by Aspergillus niger in solid state fermentation

Acta Scientiarum Technology ◽

10.4025/actascitechnol.v42i1.41358 ◽

2019 ◽

Vol 42 ◽

pp. e41358

Author(s):

Fernanda de Oliveira ◽

Marcelo Rodrigues de Melo ◽

João Batista Buzato

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Rice Straw ◽

Sugarcane Bagasse ◽

Solid State Fermentation ◽

Industrial Waste ◽

Cellulose Degradation ◽

Maximum Activity ◽

Soybean Hulls ◽

Industrial Residues

The low-cost production of cellulolytic complexes that present high action at mild conditions is one of the major bottlenecks for the economic viability of the production of cellulosic ethanol. The influence of agro-industrial residues was assessed to enhance endoglucanase production by Aspergillus niger 426 grown in solid state fermentation. The highest percentage of lignin degradation was found on soybean hulls (56%) followed by sugarcane bagasse (36%) and rice straw (8.5%). The cellulose degradation, around 90%, was observed on soybean hulls and sugarcane bagasse, but only 50% on rice straw, and maximum production of endoglucanase (112.34 ± 0.984 U mL-1) was observed for soybean hulls. The best Experimental Mixture Design condition was under cultivation of 2.5 g of sugarcane bagasse, 2.3 g of rice straw and 5.2 g of soybean hulls, leading to a maximum activity of 138.92 ± 0.02 U mL-1. The statistical methodology enabled an increase of over 20% in the production of endoglucanase using agro-industrial waste. These data demonstrate that A. niger 426 is a potential source of cellulases which can be obtained by solid state fermentation using agro-industrial waste.

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Optimization of the Production of α-L-Rhamnosidase by Aspergillus niger in Solid State Fermentation Using Agro-Industrial Residues

British Microbiology Research Journal ◽

10.9734/bmrj/2014/6862 ◽

2014 ◽

Vol 4 (11) ◽

pp. 1198-1210 ◽

Cited By ~ 1

Author(s):

Adriana Petri

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Industrial Residues

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Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes

Jurnal Biodjati ◽

10.15575/biodjati.v4i1.4411 ◽

2019 ◽

Vol 4 (1) ◽

pp. 105-111

Author(s):

Kahar Muzakhar ◽

Rudju Winarsa

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Maximum Activity ◽

Reducing Sugar ◽

Sugar Production ◽

Coffee Pulp ◽

Alkali Extract ◽

Ph Range ◽

Hydrolysis Activity

An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C.

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PRODUCTION OF LIGNOCELLULOSES FROM ASPERGILLUS NIGER USING ARECA NUT SPADE AS BIOMASS..

10.36106/ijsr/1515973 ◽

2019 ◽

pp. 1-3

Author(s):

Madhuri B ◽

Narasimha G ◽

Balaji M*

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Cellulase Production ◽

Areca Nut ◽

Physical Treatment ◽

Cellulose Production ◽

Lactobionic Acid ◽

Cellulase Enzyme

Areca palm (ChrysalidoCarpus lutescenes) a widely used plant having feathery arching brands with 100 leaflets. All these plants produce much of waste in additions to greeny and nuts. This waste of spade is used for the production of various molecules that are used in industry and pharma sector. Fermentation techniques are used to generate economically important enzymes for industrial and pharmaceutical purposes. Cellulase enzyme degrades the cellulose in between β-1, 4 glucosidic link found in lignocellulosic complex which under physical treatment is slower to degrade. The present study of Aspergillus niger for cellulose production was carried in solid state (SS) and submerged (SM) fermentations for production of cellulase enzyme. Cellulase production in SSF after 72 h of fermentation was 8.02 and in SMF activity was 2.98 per ml of cultured broth at H 6 and temperature at 30°C. Both SMF and SSF were supplemented with lactose and lactobionic acid, which acted as cellulase P production inducers. The aim of the present work was to study the effect of Areca palm spade as substrate for Aspergillus niger and its cellulase production under SMF and SSF.

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Agro-Industrial Residues as Solid Substrate for α-Amylase Production Using Solid State Fermentation by Filamentous Fungi: A Review

Bioscience Biotechnology Research Communications ◽

10.21786/bbrc/13.2/28 ◽

2020 ◽

Vol 13 (2) ◽

pp. 550-555

Author(s):

Santosh Kumar Mishra

Keyword(s):

Solid State ◽

Filamentous Fungi ◽

Solid State Fermentation ◽

Solid Substrate ◽

Industrial Residues ◽

Amylase Production

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Food Waste-Assisted Metal Extraction from Printed Circuit Boards: The Aspergillus niger Route

Microorganisms ◽

10.3390/microorganisms9050895 ◽

2021 ◽

Vol 9 (5) ◽

pp. 895

Author(s):

Carlotta Alias ◽

Daniela Bulgari ◽

Fabjola Bilo ◽

Laura Borgese ◽

Alessandra Gianoncelli ◽

...

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Food Waste ◽

Solid State Fermentation ◽

Printed Circuit Boards ◽

Electronic Waste ◽

Metal Extraction ◽

Circuit Boards ◽

Printed Circuit ◽

Waste Printed Circuit Boards

A low-energy paradigm was adopted for sustainable, affordable, and effective urban waste valorization. Here a new, eco-designed, solid-state fermentation process is presented to obtain some useful bio-products by recycling of different wastes. Urban food waste and scraps from trimmings were used as a substrate for the production of citric acid (CA) by solid state fermentation of Aspergillus niger NRRL 334, with a yield of 20.50 mg of CA per gram of substrate. The acid solution was used to extract metals from waste printed circuit boards (WPCBs), one of the most common electronic waste. The leaching activity of the biological solution is comparable to a commercial CA one. Sn and Fe were the most leached metals (404.09 and 67.99 mg/L, respectively), followed by Ni and Zn (4.55 and 1.92 mg/L) without any pre-treatments as usually performed. Commercial CA extracted Fe more efficiently than the organic one (123.46 vs. 67.99 mg/L); vice versa, biological organic CA recovered Ni better than commercial CA (4.55 vs. 1.54 mg/L). This is the first approach that allows the extraction of metals from WPCBs through CA produced by A. niger directly grown on waste material without any sugar supplement. This “green” process could be an alternative for the recovery of valuable metals such as Fe, Pb, and Ni from electronic waste.

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