Indirect Determination of Acetylcysteine in Pharmaceutical Sample by Discoloration Spectrophotometry Using Fe(III)-Tiron System

2015 ◽  
Vol 713-715 ◽  
pp. 2710-2713
Author(s):  
Chang Qing Tu ◽  
Xin Rong Wen
Keyword(s):  
Spectrophotometric Method ◽  
Indirect Determination ◽  
Chromogenic Reagent ◽  
Pharmaceutical Sample

Fe (Ш) can be reduced to Fe (II) by hydrosulfuryl (-SH) in acetylcysteine molecule,and then using tiron as chromogenic reagent of Fe (Ш),and the content of acetylcysteine is determinated indirectly through determinating the surplus content of Fe (Ш) in the system.An accurate fast spectrophotometric method for the determination of acetylcysteine by discoloration spectrophotometry using Fe (Ш)-tiron system has been established.This proposed method had been successfully applied to determinate of acetylcysteine in real pharmaceutical.

scholarly journals Sensitive Spectrophotometric Determinations of Paracetamol and Protriptyline HCl Using 3-Chloro-7-hydroxy-4-methyl-2H-chromen-2-one

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Kumble Divya ◽  
Badiadka Narayana ◽  
Majal Sapnakumari
Keyword(s):  
Charge Transfer ◽  
Spectrophotometric Method ◽  
Charge Transfer Complex ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pharmaceutical Samples ◽  
Chromogenic Reagent ◽  
Detection And Quantification

A new spectrophotometric method is developed for the determination of Paracetamol (PCT) and protriptyline HCl (PTP) in pure forms and in pharmaceutical formulations. The experiment involves the use of 3-chloro-7-hydroxy-4-methyl-2H-chromen-2-one as a novel chromogenic reagent for the determination of PCT and PTP. The method is based on the formation of charge transfer complex between the drugs and chromogenic reagent. Beer's law is obeyed in the concentration ranges 10.00–60.00 µg mL−1 for PCT at 545 nm and 40.00–160.00 µg mL−1 for PTP at 468 nm. The molar absorptivity, Sandell, sensitivity, and limit of detection and quantification are also calculated. The method has been successfully applied for the determination of both PCT and PTP in pharmaceutical samples with acceptable results.

A Simple Sensitive Spectrophotometric Method for the Determination of Ce 4+ Using Variamine Blue as a Chromogenic Reagent

2004 ◽  
Vol 144 (4) ◽  
pp. 291-295
Author(s):  
Mendalin Mathew ◽  
Badiadka Narayana ◽  
Naracham Veedu Sreekumar ◽  
Kuttamath Vipin
Keyword(s):  
Spectrophotometric Method ◽  
Chromogenic Reagent ◽  
Variamine Blue ◽  
Sensitive Spectrophotometric Method

Indirect Determination of Methimazole in Pharmaceutical Sample by Discoloration Spectrophotometry Using Fe (III)-Tiron System

2013 ◽  
Vol 781-784 ◽  
pp. 115-119 ◽  
Author(s):  
Chang Qing Tu ◽  
Xin Rong Wen
Keyword(s):  
Linear Regression ◽  
Linear Relationship ◽  
Correlation Coefficient ◽  
Linear Correlation ◽  
Effect Factors ◽  
Indirect Determination ◽  
Pharmaceutical Sample ◽  
Novel Method ◽  
Maximum Absorption Wavelength

A novel method for the indirect determination of methimazole by discoloration spectrophotometry using Fe (Ш)-tiron system has been established.The various effect factors on the determination of methimazole by discoloration spectrophotometry using tiron-Fe (Ш) system were investigated in detail. The results showed that by controlling pH=2.8,Fe (Ш) could be reduced to Fe (II) by hydrosulfuryl (-SH) in methimazole molecule,and then using tiron as chromogenic reagent of Fe (Ш),and the content of methimazole was determinated indirectly through determinating the surplus content of Fe (Ш) in the system.The maximum absorption wavelength of chromogenic system was 663 nm, good linear relationship was obtained between A and the concentration of methimazole in the range of 3.2~12.8μg·mL-1,the equation of the linear regression was A=-0.1849+57.277ρ (mg·mL-1),with a linear correlation coefficient was 0.9998.This proposed method had been successfully applied to determinate of methimazole in real pharmaceutical.

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