DNA Electrochemical Biosensor Based on Chitosan and Gold Nanoparticles Using Methylene Blue as Electrochemical Indicator

In this paper, a novel DNA electrochemical biosensor based on layer-by-layer self-assembled technology was reported. Gold nanoparticles were firstly immobilized on chitosan modified gold electrode by large amino of chitosan because of strong electrostatic adsorption effect between amino and gold atom, and thoil group modified DNA (SH-ssDNA) probe sequence was then self-assembled onto the electrode. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were applied to investigate the electrochemical behavior of different modified electrodes with methylene blue as hybrid indicator. The results in optimization experiment condition show that: The peak current difference value pre and post hybridization was linearly related to the logatithmic value of the target DNA concentration ranging from 10-8~10-5mol/L.A detection limit of 3.55×10-9mol/L can be estimated.

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DNA Electrochemical Biosensor Based on Chitosan and Core-Shell Au@SiO2 Nanoparticles

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.298.135 ◽

2011 ◽

Vol 298 ◽

pp. 135-141 ◽

Cited By ~ 1

Author(s):

Cun Zhou ◽

Ai Chun Zhang

Keyword(s):

Gold Nanoparticles ◽

Electrochemical Biosensor ◽

Gold Atom ◽

Sio2 Nanoparticles ◽

Quantitative Detection ◽

Layer By Layer ◽

Adsorption Effect ◽

Dna Electrochemical Biosensor ◽

Modified Dna ◽

Self Assembled

In this paper, an efficient process for preparing monodisperse gold nanoparticles coated with silica shells (Au@SiO2) was reported, and a novel DNA electrochemical biosensor based on layer-by-layer self-assembled technology as well as Au@SiO2 nanoparticles was presented. Chitosan was immobilized on gold electrode by good film-forming property and lots of amino because of strong electrostatic adsorption effect between amino and gold atom, Au@SiO2 nanoparticles were also immobilized to the electrode in that it kept the excellent features of gold nanoparticles, therefore, thoil group modified DNA (SH-ssDNA) probe sequence can self-assembled on Au@SiO2 nanoparticles modified electrode with the purpose of realize quantitative detection of complementary DNA. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were applied to investigate the electrochemical behavior of different modified electrodes with methylene blue as hybrid indicator. The results in optimization experiment condition show that: the peak current difference value pre and post hybridization was linearly related to the logatithmic value of the target DNA concentration ranging from 10-10~10-6mol/L. A detection limit of 4.02×10-11mol/L can be estimated.

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A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite

The Analyst ◽

10.1039/d1an00116g ◽

2021 ◽

Vol 146 (8) ◽

pp. 2679-2688

Author(s):

Chammari Pothipor ◽

Noppadol Aroonyadet ◽

Suwussa Bamrungsap ◽

Jaroon Jakmunee ◽

Kontad Ounnunkad

Keyword(s):

Methylene Blue ◽

Gold Nanoparticles ◽

Signal Amplification ◽

Electrochemical Biosensor ◽

Highly Sensitive ◽

Highly Dispersed ◽

Polypyrrole Composite ◽

Amplification Strategy ◽

Dispersed Gold ◽

Potential Tool

An ultrasensitive electrochemical biosensor based on a gold nanoparticles/graphene/polypyrrole composite modified electrode and a signal amplification strategy employing methylene blue is developed as a potential tool for the detection of miRNA-21.

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Gold nanoparticles modified electrode via simple electrografting of in situ generated mercaptophenyl diazonium cations for development of DNA electrochemical biosensor

Biosensors and Bioelectronics ◽

10.1016/j.bios.2010.07.076 ◽

2011 ◽

Vol 26 (5) ◽

pp. 1947-1952 ◽

Cited By ~ 27

Author(s):

Feng Li ◽

Yan Feng ◽

Pingjun Dong ◽

Limin Yang ◽

Bo Tang

Keyword(s):

Gold Nanoparticles ◽

Modified Electrode ◽

Electrochemical Biosensor ◽

Dna Electrochemical Biosensor

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DNA Biosensors Based on Layer-by-Layer Self-Assembled Multilayer Films of Carbon Nanotubes and Gold Nanoparticles

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.298.84 ◽

2011 ◽

Vol 298 ◽

pp. 84-84 ◽

Cited By ~ 1

Keyword(s):

Carbon Nanotubes ◽

Gold Nanoparticles ◽

Multilayer Films ◽

Layer By Layer ◽

Dna Biosensors ◽

Self Assembled ◽

Double Publication

The paper has been withdrawn do to double publication. The original was published by SPIE Proceedings of SPIE, vol 7493, 74935A in 2009 (DOI:10.1117/12.840316)

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