Characteristics and the Basic Kinetics of Ammonium Removal by Heterotrophic Nitrifying Bacteria at Low Temperature

2013 ◽  
Vol 807-809 ◽  
pp. 327-331
Author(s):  
Xiao Fei Huang ◽  
Wei Guang Li ◽  
Duo Ying Zhang ◽  
Wen Qin

A oligotrophic heterotrophic nitrifying bacterium Y21 was isolated from Songhua River at low temperature. The influence of temperature, pH, rotating speed of shaker as well as the carbon sources on the strain Y21 was investigated in this study. The experimental results showed that for the strain Y21, the optimum conditions were found to be temperature of 15 °C, pH of 7.4~8.2, shaking speed of 140 rmp respectively, with sodium acetate as carbon source. The strain Y21 has high activity under the culture condition at low temperature. The basic dynamic equation for the degradation of ammonia-nitrogen was obtained.

2013 ◽  
Vol 726-731 ◽  
pp. 406-411 ◽  
Author(s):  
Duo Ying Zhang ◽  
Wei Guang Li ◽  
Wen Qin ◽  
Xiao Fei Huang

For reducing ammonium concentration and guaranteeing safe drinking water, three heterotrophic bacteria were isolated from the Songhua River, which were SFA6, SFA7 and SFA11. When the initial ammonium concentration was about 130 mg/L, the ammonium removal rate of the strains SFA7, SFA6 and SFA 11 were 1.54 mg NH4+/L/h, 1.20 mg NH4+/L/h and 1.27 mg NH4+/L/h respectively at 8 °C. The 16S rDNA sequence results revealed that the strain SFA6 wasBacillus subtilis, SFA7 wasPseudomonas putida, and SFA11 showed similarity toPseudomonas nitroreducens. The biochemical characteristics of SFA6 were quite different from that of SFA7 and SFA11. After 48 h degradation, the NH4+-N (about 5 mg/L) was consumed to 0.23±0.15 mg/L, 0.37±0.20 mg/Land 0.58±0.17 mg/L by the strains SFA6, SFA7 and SFA11, with little NO3--N and NO2--N accumulation. Above all, the strains SFA6, SFA7 and SFA11 could be used in drinking water treatment at 8 °C. The strain SFA6 showed the highest ammonium removal efficiency.


2021 ◽  
Author(s):  
Hui Liu ◽  
Junyu Liang ◽  
Giorgos Markou ◽  
Zhaofeng Song ◽  
Jianfeng Ye

Abstract Swine wastewater (SW) poses a great threat to the environment due to its high-nutrient profiles if not properly managed. Advanced biological treatment method is an efficient method to treat SW by screening potent microalgae or bacterial strains. In this study, activated sludge, nine locally isolated heterotrophic nitrification bacteria and one microalgal strain (Chlorella) were used as inoculums in treating a local SW. Their treatment efficiencies were compared, while the nitrogen removal mechanisms and microbiome profile were explored in detail. It was found that certain heterotrophic nitrification strains had a slight advantage in removing chemical oxygen demand and phosphorus from SW, with the highest removal efficiencies of 83.9% and 76.2%, respectively. The removal efficiencies of ammonia nitrogen and total nitrogen in wastewater by microalgae reached 80.9% and 66.0% respectively, which were far higher than all the heterotrophic nitrification strains. Biological assimilation was the main pathway of nitrogen conversion by microalgae and heterotrophic nitrifying bacteria; especially microalgae showed excellent biological assimilation performance. Correlation analysis showed that Comamonas was highly positively correlated with nitrogen assimilation, while Acidovorax was closely correlated with simultaneous nitrification and denitrification. This study gives a comparison of microalgae and heterotrophic nitrifying bacteria on the nitrogen transfer and transformation pathways.


2011 ◽  
Vol 183-185 ◽  
pp. 720-724 ◽  
Author(s):  
Ping Li ◽  
Li Long Yan ◽  
Fang Ma

Biological Aerated Filter has the drawback of severe plug under low temperature, and frequent back-washing would cause the bad performance of ammonia removal. To solve these shortcomings, partial backwashing experiment was carried out to test its amelioration effect on Biological Aerated Filter. The result showed that performing backwashing at the 40 cm of filter had strong protective effect on nitrifying bacteria, the ammonia removal could be improved gradually with the highest removal rate of 71.71%. Partial backwashing affected less on ammonia removal and the removal efficiency could be restored to the previous level after the backwashing completed for 2.5 h.


2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Chaoxu Wang ◽  
Jing Ren ◽  
Xin Qiao ◽  
Mudassir Habib

In order to explore the performance of biochar-based microbial immobilization body in ammonium removal from water and potential mechanisms, a strain of heterotrophic nitrifying bacteria (HNB) was isolated from activated sludge, and the biochemical and molecular biological identification of HNB was carried out. Moreover, HNO<sub>3</sub>-, Mg<sup>2+</sup>-, NaOH<sub>-</sub>, and NaOH+Mg<sup>2+</sup>-modified rice husk-derived biochars were prepared. Then all the five kinds of biochars, including the original biochar, were used as carriers of HNB to remove NH<sub>4</sub><sup>+</sup><sub>-</sub>N from water. Results showed that HNB was classified as <i>Pseudomonas</i>, and the 72-h NH<sub>4</sub><sup>+</sup><sub>-</sub>N removal ratio of the free bacteria reached 80.24%. Compared with biochar itself, biochar-based HNB immobilization body showed a much stronger ability to remove NH<sub>4</sub><sup>+</sup><sub>-</sub>N, especially for NaOH<sub>-</sub> and NaOH+Mg<sup>2+</sup>-modified biochars. At the initial NH<sub>4</sub><sup>+</sup><sub>-</sub>N concentration of 100 mg/L and biochar addition dose of 10 g/L, NH<sub>4</sub><sup>+</sup><sub>-</sub>N removal ratio of NaOH<sub>-</sub> and NaOH+Mg<sup>2+</sup>-modified biochar-based HNB immobilization bodies reached 57.78% and 58.35% after 5 h, and reached 88.66% and 90.93% after 48 h respectively, which were obviously higher than the original, HNO<sub>3-</sub> and Mg<sup>2+</sup>-modified biochar-based HNB immobilization bodies. The phenomenon resulted from significantly higher bacteria adsorption ability of NaOH<sub>-</sub> and NaOH+Mg<sup>2+</sup>-modified biochars, which reached 773.75 and 941.17 nmol P/g biochar, respectively.


2012 ◽  
Vol 67 (2) ◽  
pp. 133-139
Author(s):  
B. Matijević ◽  
I. Kumić ◽  
T. Belić

1980 ◽  
Vol 45 (12) ◽  
pp. 3402-3407 ◽  
Author(s):  
Jaroslav Bartoň ◽  
Vladimír Pour

The course of the conversion of methanol with water vapour was followed on a low-temperature Cu-Zn-Cr-Al catalyst at pressures of 0.2 and 0.6 MPa. The kinetic data were evaluated together with those obtained at 0.1 MPa and the following equation for the reaction kinetics at the given conditions was derived: r = [p(CH3OH)p(H2O)]0.5[p(H2)]-1.3.


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