Extract of Housefly (Musca domestica) Maggot Anti-Inflammatory Parts could Regulate the Proliferation and Migration of TNF-α- Stimulated Human Umbilical Vein Endothelial Cells

House fly maggot, Musca domestica (Linnaeus) (Diptera: Muscidae) is one of the traditional Chinese medicine (TCM). In our earlier studies, the anti-inflammatory and anti-atherosclerotic functions of the housefly maggot have been found and also the anti-inflammatory effective parts have been acquired. In this study, the effect of housefly maggot anti-inflammatory parts on proliferation and migration of TNF-α-stimulated human umbilical vein endothelial cells (HUVEC) were investigated. And the results showed that the proliferation index and the migration rates of HUVEC which stimulated by TNF-α were decreased significantly in housefly maggot anti-inflammatory parts treatment group. And also the secretion of vascular endothelial growth factor (VEGF) was decreased too compared with only TNF-α treatment group. Based on the above, the housefly maggot anti-inflammatory parts could regulate the endothelial cell dysfunction through decreasing cell proliferation and migration and a reduction in VEGF expression might plays a key role in this process.

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Tentacle extract from the jellyfish Cyanea capillata increases proliferation and migration of human umbilical vein endothelial cells through the ERK1/2 signaling pathway

PLoS ONE ◽

10.1371/journal.pone.0189920 ◽

2017 ◽

Vol 12 (12) ◽

pp. e0189920 ◽

Cited By ~ 3

Author(s):

Beilei Wang ◽

Dan Liu ◽

Chao Wang ◽

Qianqian Wang ◽

Hui Zhang ◽

...

Keyword(s):

Endothelial Cells ◽

Signaling Pathway ◽

Umbilical Vein ◽

Human Umbilical Vein ◽

Cyanea Capillata ◽

And Migration ◽

Umbilical Vein Endothelial Cells ◽

Proliferation And Migration

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LncRNA TUG1 silencing enhances proliferation and migration of ox-LDL-treated human umbilical vein endothelial cells and promotes atherosclerotic vascular injury repairing via the Runx2/ANPEP axis

International Journal of Cardiology ◽

10.1016/j.ijcard.2021.05.014 ◽

2021 ◽

Author(s):

Hong Du ◽

Lei Yang ◽

Hui Zhang ◽

Xiaolin Zhang ◽

Huiyu Shao

Keyword(s):

Endothelial Cells ◽

Vascular Injury ◽

Umbilical Vein ◽

Human Umbilical Vein ◽

And Migration ◽

Umbilical Vein Endothelial Cells ◽

Lncrna Tug1 ◽

Proliferation And Migration

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Correction: GDF11/BMP11 activates both smad1/5/8 and smad2/3 signals but shows no significant effect on proliferation and migration of human umbilical vein endothelial cells

Oncotarget ◽

10.18632/oncotarget.10664 ◽

2016 ◽

Vol 7 (29) ◽

pp. 46832-46832 ◽

Cited By ~ 6

Author(s):

Yong-Hui Zhang ◽

Feng Cheng ◽

Xue-Ting Du ◽

Jin-Lai Gao ◽

Xiao-Lin Xiao ◽

...

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Human Umbilical Vein ◽

And Migration ◽

Umbilical Vein Endothelial Cells ◽

Proliferation And Migration

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The effect of formononetin on the proliferation and migration of human umbilical vein endothelial cells and its mechanism

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2018.12.049 ◽

2019 ◽

Vol 111 ◽

pp. 86-90 ◽

Cited By ~ 2

Author(s):

Chunmei Liang ◽

Anwei Zhou ◽

Chengliang Sui ◽

Zhaoquan Huang

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Human Umbilical Vein ◽

And Migration ◽

Umbilical Vein Endothelial Cells ◽

Proliferation And Migration

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Effect of Lentivirus-Mediated miR-499a-3p on Human Umbilical Vein Endothelial Cells

BioMed Research International ◽

10.1155/2020/9372961 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13

Author(s):

Huilei Zheng ◽

Juan Li ◽

Ying Chen ◽

Danping Gong ◽

Jianlin Wen ◽

...

Keyword(s):

Endothelial Cells ◽

Umbilical Vein ◽

Human Umbilical Vein ◽

Polymerase Chain ◽

And Migration ◽

Umbilical Vein Endothelial Cells ◽

Cell Migration And Proliferation ◽

Scratch Tests ◽

Proliferation And Migration

Objective. To explore the possible role of miR-499a-3p in the function of primary human umbilical vein endothelial cells (HUVECs) and the expression of ADAM10 in primary HUVEC. Method. miR-499a-3p was first transfected into primary HUVECs via lentivirus vector. The viability, proliferation, and migration of stable transfected primary HUVEC were then determined by flow cytometry, CCK8 assays, scratch tests, and Transwell tests. The transcription of miR-499a-3p and ADAM10 was examined by reverse transcription-polymerase chain reaction (RT-PCR), and the expression of ADAM10 was examined by Western blot (WB). Results. After transfection, miR-499a-3p transcription was significantly increased (P<0.01), compared to the blank and nonspecific control (NC) groups, while both ADAM10 transcription and expression were significantly decreased (P<0.05). In contrast, in the inhibitors group, miR-499a-3p transcription was significantly reduced (P<0.05) whereas both ADAM10 transcription and expression were significantly increased (P<0.05). The viability, proliferation, and migration of primary HUVECs were significantly impaired (P<0.05) by the transfection of miR-499a-3p but enhanced by miR-499a-3p inhibitors (P<0.05). Conclusions. Upregulation of miR-499a-3p transcription will inhibit the expression of ADAM10 in HUVECs; cell migration and proliferation, however, promote apoptosis. And reverse effects were established by downregulation of miR-499a-3p transcription. All these effects may be achieved by regulating the transcription and expression of ADAM10. These results combined suggested that miR-499a-3p may affect the proliferation, migration, and apoptosis of endothelial cells and regulate AS by regulating ADAM10. miR-499a-3p may become a candidate biomarker for the diagnosis of unstable angina pectoris (UA).

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