Portable-Surface Plasmon Resonance Biosensor Immunoassays for the Human Serum Albumin Detection

2013 ◽  
Vol 562-565 ◽  
pp. 408-411 ◽  
Author(s):  
Hui Li ◽  
Lu Lu Zhang ◽  
Hao Yuan Cai ◽  
Xing Chen ◽  
Jian Hai Sun ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Surface Plasmon Resonance ◽  
Human Serum Albumin ◽  
Light Intensity ◽  
Serum Albumin ◽  
Human Serum ◽  
Label Free ◽  
Detection Range ◽  
Spr Biosensor ◽  
Detection Assay

The screening of urine human serum albumin (HSA) is significant for the early diagnosis and intervention of the nephropathia. This study developed sensitive HSA detection assay with a self-made portable-SPR biosensor based on the reflection light intensity interrogation. Anti-HSA monoclonal antibody was immobilized onto the gold sensing area and a directive assay for HSA was developed. A detection range of 1–100μg/mL HSA was found. The described label-free, real-time immunoassay for the quantification of HSA in urine is a rapid, sensitive, alternative method available for the clinic detection.

Drug/Antibody Conjugates. In Vitro Cytotoxicity of a Human Serum Albumin-Mediated Conjugate of Methotrexate with Anti-MM46 Monoclonal Antibody

1987 ◽  
Vol 29 (4) ◽  
pp. 566-568
Author(s):  
Noriaki Endo ◽  
Yoshinori Kato ◽  
Yumiko Takeda ◽  
Masahiko Saito ◽  
Naoji Umemoto ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
In Vitro Cytotoxicity ◽  
Antibody Conjugates

scholarly journals Monitoring human serum albumin cell cultures using surface plasmon resonance (SPR) spectroscopy

2015 ◽  
Vol 4 (1) ◽  
pp. 77-83 ◽  
Author(s):  
A. Henseleit ◽  
C. Pohl ◽  
Th. Bley ◽  
E. Boschke
Keyword(s):  
Surface Plasmon Resonance ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Surface Plasmon ◽  
Cell Cultures ◽  
Plasmon Resonance ◽  
Incident Light ◽  
Three Dimensional ◽  
Critical Parameters

Abstract. Continuously monitoring cell cultures is essential for both controlling critical parameters and improving understanding of key processes. An ideal technique in this context is surface plasmon resonance (SPR) spectroscopy, which essentially exploits changes in the angle of incident light that occur when molecules bind to a surface. It provides the ability to monitor real-time changes in small concentrations of various molecules, with no need for additional labels or sample preparation. Here we present an SPR-based immunoassay for monitoring concentrations of human serum albumin (HSA), and compare its sensitivity when used in conjunction with a Biacore platform and the cheaper, smaller liSPR system. In conjunction with either system, the immunoassay can detect HSA (a hepatocyte viability marker) at concentrations typically present in three-dimensional hepatocyte cultures mimicking the liver used to evaluate effects of drug candidates before exposure to humans or animals. Furthermore, in conjunction with the liSPR system, it is sufficiently sensitive to measure the much lower HSA levels present in skin–hepatocyte co-cultures.

A Unique Epitope on Human Serum Albumin Recognized by Monoclonal Antibody HSA-1: A Probe for Identification of the Human Origin of Blood or Tissue

Hybridoma ◽  
1987 ◽  
Vol 6 (2) ◽  
pp. 183-190 ◽  
Author(s):  
DAVID C. BENJAMIN ◽  
JOHN C. HERR ◽  
WILLIAM M. SUTHERLAND ◽  
MICHAEL P. WOODWARD ◽  
KRISTI DeCOURCY ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Human Origin
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