Production and Characterization of Bacterial Cellulose from Rice Washing Drainage (RWD) by Komagataeibacter nataicola Li1

2019 ◽  
Vol 824 ◽  
pp. 30-37
Author(s):  
Pipat Sudying ◽  
Nuchanat Laingaumnuay ◽  
Phimchanok Jaturapiree
Keyword(s):  
Carbon Source ◽  
Bacterial Cellulose ◽  
Sole Carbon Source ◽  
Yeast Fermentation ◽  
Malus Pumila ◽  
Structure And Properties ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis ◽  
Agitated Culture

Bacterial cellulose (BC), produced by some bacteria has received attention because its high purity and robust characteristics enable its use in medicine and industry. This study aimed to screen bacteria capable of producing BC from fruits, and to optimize the BC production in both traditional YE yeast fermentation and Schramm and Hestrin (SH) media. The rice washing drainage without any pretreatment as the sole carbon source in the yeast medium was also investigated for BC production in both static and agitated cultures. The bacterial strain Li1 isolated from apple (Malus pumila) was identified as Komagataeibacter (Gluconacetobacter) nataicola based on 16S rDNA sequence analysis. K. nataicola Li1 cultured in a YE medium produced significantly more BC than that in a SH medium in the static culture. Moreover, the weight yields of dry BC films obtained from rice washing drainage and YE media in the static cultures were not significantly different. However, in the agitated culture, the weight yields of dry BC films were more significant in the YE medium than in rice washing drainage medium. In addition, the structure and properties of BC were determined using SEM, FTIR, DSC and XRD. This study shows that rice washing drainage can be used as the carbon source for BC production by K. nataicola Li1.

Isolation of One Strain for Biodegradation of Diesel Fuel from Sea Water

2013 ◽  
Vol 726-731 ◽  
pp. 2424-2427
Author(s):  
Pei Pei Han ◽  
Ping Zhe Jiang ◽  
Xiang He Liu ◽  
Hong Yi Huang ◽  
Wen Qian Duan ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Carbon Source ◽  
Phylogenetic Tree ◽  
Diesel Fuel ◽  
Sole Carbon Source ◽  
Culture Medium ◽  
Sea Water ◽  
Rhodococcus Erythropolis ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

Four diesel fuel degrading microorganisms were isolated from soil and sea water from Crude Oil Terminal at Tianjin Port using diesel fuel as sole carbon source, and they all could degrade diesel fuel. The initial diesel fuel concentration in the culture medium was optimized and was 1.5 g/L. The ability of the four strains to degrade diesel was compared by spectrophotometer with the initial diesel concentration at 1.5 g/L. The results showed that the strain C1 had the highest degrading activity, which could degrade 32.59% of diesel in 7 days. The strain C1 was further identified by 16S rDNA sequence analysis as Rhodococcus erythropolis, and the phylogenetic tree of the strain C1 was constructed.

scholarly journals Erratum to: Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

2014 ◽  
Vol 52 (12) ◽  
pp. 1056-1056
Author(s):  
Ok-Hwa Hwang ◽  
Sebastian Raveendar ◽  
Young-Ju Kim ◽  
Ji-Hun Kim ◽  
Tae-Hun Kim ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Bacterial Diversity ◽  
16S Rdna ◽  
Rdna Sequence ◽  
Pig Slurry ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

scholarly journals Isolation and Characterization of a Novel p,p’-DDT-Degrading Bacterium: Aeromonas sp. Strain MY1 from Agricultural Soil

2020 ◽  
pp. 12-22
Author(s):  
Y. Murtala ◽  
B. C. Nwanguma ◽  
L. U. S. Ezeanyika
Keyword(s):  
Carbon Source ◽  
16S Rrna ◽  
Agricultural Soil ◽  
Sole Carbon Source ◽  
Phylogenetic Analyses ◽  
Inhibitory Effect ◽  
Aerobic Conditions ◽  
Degrading Bacterium ◽  
Isolation And Characterization

Background: Despite the banned on the use of dichlorodiphenyltrichloroethane (DDT) and other Persistent Organic Pollutants (POPs) by the Stockholm Convention for their toxicity, emerging shreds of evidence have indicated that DDT is, however, still in use in developing countries. This might increase the global burden of DDT contamination and its hazardous effects. Aim: This study focused on the isolation and characterization of p,p’-DDT-degrading bacterium from a tropical agricultural soil. Methodology: Standard isolation procedure was used for the screening and isolation of the strain. The 16S rRNA and phylogenetic analyses were used to identify the isolate and established protocols were followed to characterize the strain. Results: A new strain belonging to the genus Aeromonas was isolated from agricultural soil using minimal salt-p,p’-DDT enrichment medium. The 16S rRNA sequencing was used to identify the strain and the partial sequence was deposited in the NCBI GenBank as Aeromonas sp. Strain MY1. This mesophilic isolate was capable of utilizing up to 50 mgL-1 of p,p’-DDT as the sole carbon source at an optimum pH of 7.5 and optimum temperature of 35 °C within 120 h under aerobic conditions. Fe2+ (0.2 mgL-1) demonstrated a stimulatory effect on the p,p’-DDT degradation capacity by the strain MY1. However, Zn, Cu, Pb, Hg, Ag and Cr ions have demonstrated various patterns of inhibitory effect on the p,p’-DDT degradation capacity of the isolate at 0.2 mgL-1. The strain MY1 could be a promising candidate for the bioremediation of p,p’-DDT contaminant. Conclusion: Aeromonas sp. strain MY1 was capable of utilizing p,p’-DDT as a sole carbon source under aerobic conditions. The utilization capacity of the strain was influenced by some heavy metals. Fe was found to enhance the p,p’-DDT utilization capacity of the isolate at a lower concentration. While Zn, Cu, Pb, Hg, Ag and Cr showed various patterns of inhibitory effect.

SEM and TEM for structure and properties characterization of bacterial cellulose/hydroxyapatite composites

Scanning ◽  
2016 ◽  
Vol 38 (6) ◽  
pp. 757-765 ◽  
Author(s):  
Natalia A. Arkharova ◽  
Elena I. Suvorova ◽  
Alexander V. Severin ◽  
Albert K. Khripunov ◽  
Sergey V. Krasheninnikov ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Structure And Properties ◽  
Sem And Tem ◽  
Properties Characterization

scholarly journals Isolation and enzymatic characterization of fungal strains capable to use polyurethane as sole carbon source

2009 ◽  
Vol 25 ◽  
pp. S69
Author(s):  
A. Loredo-Treviño ◽  
J.A. Sánchez-Vasquez ◽  
R. Rodríguez-Herrera ◽  
C. Aguilar
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Enzymatic Characterization ◽  
Fungal Strains

scholarly journals Production and Characterization of Polyhydroxybutryrate Biopolymer from Azohydromonas australica Using Sucrose as a Sole Carbon Source

2017 ◽  
Vol 09 (03) ◽  
Author(s):  
Salman Ahmad ◽  
Abuzer Amir ◽  
Md Zafaryab ◽  
Khwaja Osama ◽  
Soban Ahmad Faridi ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Azohydromonas Australica
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