Construction of Two-Gene Modified Artificial Bone by Using Recombinant Adenovirus

2009 ◽  
Vol 610-613 ◽  
pp. 1343-1355
Author(s):  
Chun Guang Duan ◽  
Guo Lin Meng ◽  
Jian Liu ◽  
Yun Yu Hu ◽  
Bao Feng Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Supply ◽  
High Efficiency ◽  
Biological Function ◽  
Recombinant Adenovirus ◽  
Artificial Bone ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Good Biocompatibility ◽  
Osseous Defects

Large osseous defects are difficult to treat because of deficient blood supply on the defected area. To get sufficient blood supply, we designed to establish the adenovirus simultaneously encoding both VEGF and Ang-1 (pAd-VIA) to accelerate the formation of new vessels in the process of bone defect repair. The construction of the adenovirus was performed according to the method reported by Tong-Chuan HE with a tiny modification. Three kinds of adenoviruses were acquired. They are adenovirus pAd-VIA simultaneously encoding VEGF and Ang-1, adenovirus pAd-VEGF encoding VEGF, and adenovirus pAd-Ang-1 encoding Ang-1. The adenovirus prepared in this study could successfully transfer VEGF and Ang-1 into mesenchymal stem cells(MSCs) with high efficiency. Two-gene modified artificial bone was established by use of these adenovirus. In the end, the two-gene modified artificial bone was proved to have good biocompatibility and biological function. Study reports presented here will pave the way for further exploration of vascularization in the process of large osseous defects repair.

scholarly journals Cobalt-doped bioceramic scaffolds fabricated by 3D printing show enhanced osteogenic and angiogenic properties for bone repair

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Jungang Li ◽  
Chaoqian Zhao ◽  
Chun Liu ◽  
Zhenyu Wang ◽  
Zeming Ling ◽  
...  
Keyword(s):  
Compressive Strength ◽  
3D Printing ◽  
Bone Regeneration ◽  
Doping Concentration ◽  
Artificial Bone ◽  
Bone Defect Repair ◽  
Co Doping ◽  
Defect Repair ◽  
Co Doped

Abstract Background The bone regeneration of artificial bone grafts is still in need of a breakthrough to improve the processes of bone defect repair. Artificial bone grafts should be modified to enable angiogenesis and thus improve osteogenesis. We have previously revealed that crystalline Ca10Li(PO4)7 (CLP) possesses higher compressive strength and better biocompatibility than that of pure beta-tricalcium phosphate (β-TCP). In this work, we explored the possibility of cobalt (Co), known for mimicking hypoxia, doped into CLP to promote osteogenesis and angiogenesis. Methods We designed and manufactured porous scaffolds by doping CLP with various concentrations of Co (0, 0.1, 0.25, 0.5, and 1 mol%) and using 3D printing techniques. The crystal phase, surface morphology, compressive strength, in vitro degradation, and mineralization properties of Co-doped and -undoped CLP scaffolds were investigated. Next, we investigated the biocompatibility and effects of Co-doped and -undoped samples on osteogenic and angiogenic properties in vitro and on bone regeneration in rat cranium defects. Results With increasing Co-doping level, the compressive strength of Co-doped CLP scaffolds decreased in comparison with that of undoped CLP scaffolds, especially when the Co-doping concentration increased to 1 mol%. Co-doped CLP scaffolds possessed excellent degradation properties compared with those of undoped CLP scaffolds. The (0.1, 0.25, 0.5 mol%) Co-doped CLP scaffolds had mineralization properties similar to those of undoped CLP scaffolds, whereas the 1 mol% Co-doped CLP scaffolds shown no mineralization changes. Furthermore, compared with undoped scaffolds, Co-doped CLP scaffolds possessed excellent biocompatibility and prominent osteogenic and angiogenic properties in vitro, notably when the doping concentration was 0.25 mol%. After 8 weeks of implantation, 0.25 mol% Co-doped scaffolds had markedly enhanced bone regeneration at the defect site compared with that of the undoped scaffold. Conclusion In summary, CLP doped with 0.25 mol% Co2+ ions is a prospective method to enhance osteogenic and angiogenic properties, thus promoting bone regeneration in bone defect repair.

Nano-Hydroxyapatite Scaffold Based on Recombinant Human Bone Morphogenetic Protein 2 and Its Application in Bone Defect Repair

2021 ◽  
Vol 17 (7) ◽  
pp. 1330-1338
Author(s):  
Shibai Zhu ◽  
Xiaotian Zhang ◽  
Xi Chen ◽  
Yiou Wang ◽  
Shanni Li ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Defects ◽  
Bone Morphogenetic Protein 2 ◽  
Artificial Bone ◽  
Bone Defect Repair ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Defect Repair ◽  
Hydroxyapatite Scaffold ◽  
Recombinant Human Bone

The best way in which to prepare scaffolds with good biological properties is an urgent problem in the field of tissue engineering. In this paper we discuss the preparation of nano-hydroxyapatite scaffold of recombinant human bone morphogenetic protein-2 (rhBMP-2) and its application in bone defect repair. rhBMP-2 reagent was dissolved in 1 mol/L sodium dihydrogen phosphate solution, and the rhBMP-2 solution was added to the nano-hydroxyapatite artificial bone with a 100 μL glass micro dropper at the rate of 10 drops/min to obtain Nano-HA/rhBMP-2 composite artificial bone. In in vivo experiments, rabbits were fixed on an operating table, a 2 cm longitudinal incision was made in the middle part of the radial forearm, and the radius was cut with a wire saw and periosteum, 2.5 cm away from the distal radius. After washing the wound with normal saline, Adv-hBMP-2/MC3T3-E1 nano-HA composite artificial bone, MC3T3-E1 nan-HA composite artificial bone, or Nano-HA artificial bone were implanted in different groups. The artificial bone scaffold prepared in this study has a stronger ability to repair bone defects than the alternatives, and is a promising prospect for the clinical treatment of bone defects.

scholarly journals Tissue engineering using mesenchymal stem cells with periosteal wrap for bone defect repair in rabbits

2015 ◽  
Vol 2 (6) ◽  
pp. 340
Author(s):  
Trung-Hau Lê Thua ◽  
Dang-Nhat Pham ◽  
Khanh-Linh Lê ◽  
Minh-Tuan Lê ◽  
Quang-Ton-Quyen Nguyen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Bone Defect Repair ◽  
Defect Repair

An in situ phototriggered-imine-crosslink composite hydrogel for bone defect repair

2016 ◽  
Vol 4 (5) ◽  
pp. 973-981 ◽  
Author(s):  
Jieyuan Zhang ◽  
Yunlong Yang ◽  
Yunfeng Chen ◽  
Xiaolin Liu ◽  
Shangchun Guo ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Repair ◽  
Composite Hydrogel ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Good Biocompatibility

A novel in situ formed composite hydrogel based on the phototriggered imine crosslink mechanism with good biocompatibility and osteoinduction is developed for bone repair.

scholarly journals Ferulic acid promotes bone defect repair after radiation by maintaining the stemness of skeletal stem cells

2021 ◽  
Author(s):  
Jia‐Wu Liang ◽  
Pei‐Lin Li ◽  
Qian Wang ◽  
Song Liao ◽  
Wei Hu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ferulic Acid ◽  
Bone Defect ◽  
Bone Defect Repair ◽  
Defect Repair
Sign in / Sign up

Export Citation Format

Share Document