Paper Chromatography to Detect Predation on Mites

1965 ◽  
Vol 97 (4) ◽  
pp. 435-441 ◽  
Author(s):  
W. L. Putman
Keyword(s):  
Paper Chromatography ◽  
Filter Paper ◽  
Pigment Content ◽  
Panonychus Ulmi ◽  
Circular Chromatography

Abstract Predators that have fed on Panonychus ulmi (Koch) and Bryobia arborea Morgan and Anderson can be distinguished by the orange and reddish carotenoid biochromes derived from the prey. Predation on the two species of mites cannot be clearly separated. The predators are squashed on filter paper and the pigments separated by circular chromatography in a mixture of one part of zylene to four parts of white kerosene. The mite pigments are considerably altered during digestion in the predators. The method does not reveal the number of mites consumed by a predator, owing to differences in the pigment content of the various stages of the prey and in the time the pigments are retained in the predators.

PAPER CHROMATOGRAPHY OF COMPLEX BIOLOGICAL MATERIALS BY SOLVENT REDEVELOPMENT WITHOUT PRIOR REMOVAL OF SALTS OR PROTEINS

1960 ◽  
Vol 38 (10) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Complex Mixtures ◽  
Two Dimensional ◽  
One Dimensional ◽  
Equivalent Time ◽  
Biological Compounds ◽  
High Concentrations ◽  
Subsequent Staining

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

Identification of Flavonoid Compounds by Filter Paper Chromatography

1951 ◽  
Vol 23 (11) ◽  
pp. 1582-1585 ◽  
Author(s):  
Thomas Gage ◽  
Carl Douglass ◽  
Simon Wender
Keyword(s):  
Paper Chromatography ◽  
Filter Paper ◽  
Flavonoid Compounds

A TECHNIQUE OF PREPARATIVE PAPER CHROMATOGRAPHY

1966 ◽  
Vol 44 (2) ◽  
pp. 149-153 ◽  
Author(s):  
A. E. Pasieka ◽  
J. E. Logan
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Biological Materials ◽  
High Salt ◽  
Salt Medium ◽  
Preparative Scale ◽  
High Concentrations ◽  
Total Amino Acids

The use of a solvent redevelopment technique enables the separation of amino acids from complex biological materials in the presence of high concentrations of salts. By conventional chromatography, 1.17 mg of total amino acids have been separated from the high salt medium M 150. The preparative technique as described here has separated amounts as great as 1.17 g and the patterns are essentially the same as for the analytical types. The separations are effected by four or more successive 15- to 20-hour solvent developments with drying between each solvent stage before the staining of chromatograms or isolation of particular bands. The results of these solvent developments on the preparative scale are illustrated with photographs of actual chromatograms. This technique requires thick filter paper sheets up to 4 ft in length for analytical, and particularly for preparative, chromatograms.

Improved Routine Maintenance of Nippostrongylus brasiliensis Culture

1968 ◽  
Vol 42 (1-2) ◽  
pp. 193-198
Author(s):  
Paul Whur
Keyword(s):  
Paper Chromatography ◽  
Filter Paper ◽  
Fungal Growth ◽  
Petri Dish ◽  
Nippostrongylus Brasiliensis ◽  
Total Inhibition ◽  
Culture Maintenance ◽  
Time Required

A new method is described for the in vitro culture of the preparasitic stages of Nippostrongylus brasiliensis. It utilises the plating of faecal suspensions on to paper chromatography strips supported on non-porous Perspex slabs and has been designed primarily to facilitate savings in the time required for routine culture maintenance without lowering the quantity or quality of the larval yield. Comparison with the filter paper/Petri dish method shows an increase in larval yield of 85% and a reduction in time required for culture of 72%. Total inhibition of fungal growth on incubated faeces suspensions was obtained by the addition of “Mycostatin” (Squibb) in a concentration of 62 units per ml. or more.

Buffered Filter Paper Chromatography of Amino Acids

1951 ◽  
Vol 23 (1) ◽  
pp. 168-174 ◽  
Author(s):  
E. F. McFarren
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper

Filter-Paper Chromatography: Extraction of Sugars from the Paper at Room Temperature

Nature ◽  
1950 ◽  
Vol 166 (4220) ◽  
pp. 476-477 ◽  
Author(s):  
R. A. LAIDLAW ◽  
S. G. REID
Keyword(s):  
Paper Chromatography ◽  
Filter Paper ◽  
Room Temperature

PAPER CHROMATOGRAPHY OF COMPLEX BIOLOGICAL MATERIALS BY SOLVENT REDEVELOPMENT WITHOUT PRIOR REMOVAL OF SALTS OR PROTEINS

1960 ◽  
Vol 38 (1) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Complex Mixtures ◽  
Two Dimensional ◽  
One Dimensional ◽  
Equivalent Time ◽  
Biological Compounds ◽  
High Concentrations ◽  
Subsequent Staining

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

PREVALENCE OF SPIDERS AND THEIR IMPORTANCE AS PREDATORS IN ONTARIO PEACH ORCHARDS

1967 ◽  
Vol 99 (2) ◽  
pp. 160-170 ◽  
Author(s):  
W. L. Putman
Keyword(s):  
Population Density ◽  
Paper Chromatography ◽  
Panonychus Ulmi ◽  
Single Tree ◽  
Minor Peak ◽  
Satisfactory Method ◽  
Form Part ◽  
Mite Density ◽  
A Minor

AbstractPhilodromus spp., especially praelustris Keyserlmg, and sometimes Theridion murarium were the only abundant spiders in peach orchards, though small numbers of many other species were present. No satisfactory method of sampling the population density of all species was discovered. The spider population as a whole reached a minor peak in June and a much higher peak in early August. A single tree may contain more than 200 spiders. When fed only on Panonychus ulmi (Koch), young P. praelustris eventually died without moulting; young of T. murarium moulted once or twice but their development was slower than that of those in the orchards. Chironomids were the commonest prey of P. praelustris and apparently of other spiders in the orchards.Spiders collected from peach plots with different densities of P. ulmi and Bryobia arborea Morgan and Anderson were examined for pigments of these mites by paper chromatography. Some spiders in all collections had fed on the mites, and the percentage that had done so usually varied directly with mite density in the plots. Spiders form part of the complex of minor predators that aid the major predators in regulating the density of P. ulmi at endemic levels.

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