Colorimetric Method for Determination of Amino Acids on Thin Layer and Filter Paper Chromatography Using a Modified Ninhydrin Reagent

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

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Thin-layer and paper chromatography of dinitrophenyl derivatives of amino acids from bacterial cell wall peptidoglycans

Journal of Chromatography A ◽

10.1016/0021-9673(70)80019-x ◽

1970 ◽

Vol 47 ◽

pp. 124-126 ◽

Cited By ~ 3

Author(s):

W.D. Grant ◽

A.J. Wicken

Keyword(s):

Amino Acids ◽

Cell Wall ◽

Thin Layer ◽

Paper Chromatography ◽

Bacterial Cell ◽

Bacterial Cell Wall ◽

Derivatives Of

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Determination of amino acids by ion-exchange chromatography on filter paper spotted blood samples stored at different temperatures and for different periods: comparison with capillary and venous blood

Clinical Biochemistry ◽

10.1016/s0009-9120(01)00244-2 ◽

2001 ◽

Vol 34 (5) ◽

pp. 399-406 ◽

Cited By ~ 11

Author(s):

Yousef M Abdulrazzaq ◽

Ahmed Ibrahim

Keyword(s):

Amino Acids ◽

Ion Exchange ◽

Filter Paper ◽

Venous Blood ◽

Ion Exchange Chromatography ◽

Exchange Chromatography ◽

Blood Samples ◽

Different Temperatures

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Thin-layer Chromatographic Separation and Quantitative Determination of Several Free Amino-acids of Avian Liver

Nature ◽

10.1038/1991216a0 ◽

1963 ◽

Vol 199 (4899) ◽

pp. 1216-1216 ◽

Cited By ~ 14

Author(s):

ROBERT L. SQUIBB

Keyword(s):

Amino Acids ◽

Thin Layer ◽

Quantitative Determination ◽

Chromatographic Separation ◽

Free Amino Acids ◽

Free Amino

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Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper.

Clinical Chemistry ◽

10.1093/clinchem/26.8.1198 ◽

1980 ◽

Vol 26 (8) ◽

pp. 1198-1200 ◽

Cited By ~ 3

Author(s):

A P Orfanos ◽

E W Naylor ◽

R Guthrie

Keyword(s):

Filter Paper ◽

Colorimetric Method ◽

Dried Blood Spots ◽

Arginase Activity ◽

Kinetic Reaction ◽

Hematological Disorders ◽

Blood Spots ◽

Blood Specimens ◽

The Stability

Abstract We describe a microfluorometric method for determination of arginase activity in dried blood spots on filter paper. The arginase in discs punched from such dried blood specimens is activated by preincubation with Mn2+ at 37 degrees C. After incubation with substrate at the same temperature, urea is determined fluorometrically by oxidation of NADH to NAD+ in a coupled kinetic reaction. We compare the results of this method with those of a colorimetric method involving liquid blood samples, and assess the stability of the enzyme in dried blood on filter paper. The presence of serum has no effect on the activity. This method may be useful in the early detection of arginase deficiency and certain hematological disorders.

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Serum Propylthiouracil: Determination by a Direct Colorimetric Procedure

Clinical Chemistry ◽

10.1093/clinchem/18.11.1373 ◽

1972 ◽

Vol 18 (11) ◽

pp. 1373-1375 ◽

Cited By ~ 19

Author(s):

Charles R Ratliff ◽

Paul F Gilliland ◽

Frank F Hall

Keyword(s):

Filter Paper ◽

Colorimetric Method ◽

Control Sample ◽

Chloroform Solution ◽

Soluble Compound ◽

Aqueous Layer ◽

Free Serum ◽

Drug Free ◽

Colorimetric Procedure

Abstract A direct colorimetric method is described for the determination of propylthiouracil in serum, which is based on reaction of the drug at pH 8.0 with 2,6-dichloroquinone-chloroimide to produce a colored chloroform-soluble compound. After isolation from the aqueous layer with phase-separating filter paper, the chloroform solution is read against a chloroform blank at 435 nm. Propylthiouracil added to drug-free serum serves as a control sample. The method obeys Beer's law up to a concentration of at least 10 mg/liter.

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