scholarly journals Bacterial DNA Activates Endothelial Cells and Promotes Neutrophil Adherence through TLR9 Signaling

2009 ◽  
Vol 182 (7) ◽  
pp. 4386-4394 ◽  
Author(s):  
Driss El Kebir ◽  
Levente József ◽  
Wanling Pan ◽  
Lili Wang ◽  
János G. Filep
Keyword(s):  
Endothelial Cells ◽  
Bacterial Dna ◽  
Neutrophil Adherence ◽  
Tlr9 Signaling

scholarly journals Thrombin-mediated release of lipids from pulmonary artery endothelial cells promotes neutrophil adherence.

1991 ◽  
Vol 68 (4) ◽  
pp. 930-939 ◽  
Author(s):  
M A Fisher ◽  
P J Del Vecchio ◽  
G P Palace ◽  
E M Denholm ◽  
L Lai ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Pulmonary Artery ◽  
Neutrophil Adherence

Exogenous xanthine promotes neutrophil adherence to cultured endothelial cells

1997 ◽  
Vol 273 (2) ◽  
pp. G342-G347
Author(s):  
H. Ichikawa ◽  
R. E. Wolf ◽  
T. Y. Aw ◽  
N. Ohno ◽  
L. Coe ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tissue Injury ◽  
Umbilical Vein ◽  
Platelet Activating Factor ◽  
Adhesion Assay ◽  
Neutrophil Adherence ◽  
Paf Receptor ◽  
Umbilical Vein Endothelial Cells ◽  
Adhesive Effect ◽  
Paf Receptor Antagonist

Oxidants generated by endothelial xanthine oxidase (XO) can help trigger free radical-mediated tissue injury. An important event in oxidant-mediated tissue injury is neutrophil-endothelial adhesion. Although activation of endothelial XO increases adhesion, little is known about xanthine in the adhesive effect of XO. This study examined administered xanthine on the adhesion of neutrophils. Endothelial [human umbilical vein endothelial cells (HUVEC)] monolayers were exposed to xanthine (15 min), and neutrophils were allowed to adhere to HUVEC in an adhesion assay. Adhesion was dose dependently increased by xanthine (3-100 microM). Either catalase (1,000 U/ml), oxypurinol (XO inhibitor; 100 microM), or platelet-activating factor (PAF) receptor antagonist (WEB 2086; 10 microM) reduced neutrophil adhesion. Superoxide dismutase (1,000 U/ml) had no effect. Pretreatment of HUVEC with 50 microM tungsten also blocked xanthine-induced adherence. Adhesion was also inhibited by preincubation with 100 U/ml heparin. Finally, anti-P-selectin antibody (PB1.3; 20 micrograms/ml) attenuated adhesion. Our results indicate that xanthine may promote neutrophil-endothelial adhesion via a hydrogen peroxide- and PAF-mediated P-selectin expression.

The Anti-Inflammatory Drug Nimesulide Inhibits Neutrophil Adherence to and Migration Across Monolayers of Cytokine-Activated Endothelial Cells

Respiration ◽  
1994 ◽  
Vol 61 (6) ◽  
pp. 336-341 ◽  
Author(s):  
Patrizia Dapino ◽  
Luciano Ottonello ◽  
Franco Dallegri
Keyword(s):  
Endothelial Cells ◽  
Inflammatory Drug ◽  
Neutrophil Adherence ◽  
Anti Inflammatory ◽  
And Migration

Mediation of endothelial injury following neutrophil adherence to extracellular matrix

1993 ◽  
Vol 264 (4) ◽  
pp. L401-L405 ◽  
Author(s):  
R. A. Kaslovsky ◽  
L. Lai ◽  
K. Parker ◽  
A. B. Malik
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Monoclonal Antibodies ◽  
Endothelial Permeability ◽  
Endothelial Injury ◽  
Matrix Proteins ◽  
Extracellular Matrix Components ◽  
The Matrix ◽  
Neutrophil Adherence ◽  
Permeability Increase

Since polymorphonuclear leukocytes (PMN) rapidly migrate across the endothelial barrier and attach to extracellular matrix components, we tested the hypothesis that adhesion of PMN to matrix proteins can mediate endothelial injury following PMN activation. Studies were made using gelatin- and fibronectin-coated polycarbonate microporous filters (10 microns thick) on which confluent monolayers of bovine pulmonary microvessel endothelial cells were grown. PMN were layered either directly onto endothelial cells (at a ratio of 10:1) (“upright system”) or onto gelatin- and fibronectin-coated filters with the endothelial monolayer grown on the underside of the filter without contact between PMN and endothelial cells (“inverted system”). PMN were activated with phorbol 12-myristate 13-acetate (PMA; 5 x 10(-9) M) in both systems. PMN activation increased endothelial permeability to 125I-labeled albumin in upright as well as inverted systems. Pretreatment of PMN with anti-CD18 monoclonal antibodies IB4 or R15.7, which inhibited PMN adherence to matrix constituents as well as to endothelial cells, prevented the permeability increase in both configurations. This effect of anti-CD18 monoclonal antibodies (mAbs) was not ascribed to a reduction in PMN activation, since PMA-induced superoxide generation was unaffected. We conclude that activation of PMN adherent to extracellular matrix proteins increases endothelial permeability to albumin and that this response is dependent on PMN adhesion to the matrix. The results support the concept that PMN-mediated increase in endothelial permeability is the result of “targeted” release of PMN products independent of whether the PMN are adherent to the extracellular matrix or the endothelium.

Redox modulation of tyrosine phosphorylation-dependent neutrophil adherence to endothelial cells

2005 ◽  
Vol 72 (2-3) ◽  
pp. 333-339
Author(s):  
Paul A. Thibodeau ◽  
Alexia Gozin ◽  
Marie-Anne Gougerot-Pocidalo ◽  
Catherine Pasquier
Keyword(s):  
Endothelial Cells ◽  
Tyrosine Phosphorylation ◽  
Redox Modulation ◽  
Neutrophil Adherence

Retention Fluids of Chronic Sinusitis Induce Neutrophil Adherence to Microvascular Endothelial Cells

1992 ◽  
Vol 112 (5) ◽  
pp. 882-889 ◽  
Author(s):  
Kazunori Itoh ◽  
Shoko Katahira ◽  
Tsutomu Matsuzaki ◽  
Masaru Ohyama ◽  
Katsunori Fukuda
Keyword(s):  
Endothelial Cells ◽  
Chronic Sinusitis ◽  
Microvascular Endothelial Cells ◽  
Neutrophil Adherence ◽  
Microvascular Endothelial

Hyperoxia Injures Endothelial Cells in Culture and Causes Increased Neutrophil Adherence

CHEST Journal ◽  
1983 ◽  
Vol 83 (5) ◽  
pp. 33S-35S ◽  
Author(s):  
C. Michael Bowman ◽  
Elaine N. Butler ◽  
Albert E. Vatter ◽  
John E. Repine
Keyword(s):  
Endothelial Cells ◽  
Cells In Culture ◽  
Neutrophil Adherence

Bacterial DNA promotes neutrophil adhesion to endothelial cells

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Levente Jozsef ◽  
Driss El Kebir ◽  
Tarek Khreiss ◽  
Janos G Filep
Keyword(s):  
Endothelial Cells ◽  
Neutrophil Adhesion ◽  
Bacterial Dna

Evidence of endothelial activation in preeclampsia: Neutrophil adherence to endothelial cells is increased in preeclamptic pregnancies

1998 ◽  
Vol 5 (1) ◽  
pp. 129A-129A
Author(s):  
Y WANG ◽  
S ALEXANDER ◽  
D ADAIR ◽  
J WEEKS ◽  
D LEWIS
Keyword(s):  
Endothelial Cells ◽  
Endothelial Activation ◽  
Neutrophil Adherence

scholarly journals Increased neutrophil adherence and adhesion molecule mRNA expression in endothelial cells during selenium deficiency

1999 ◽  
Vol 65 (5) ◽  
pp. 658-664 ◽  
Author(s):  
Jane F. Maddox ◽  
Kristen M. Aherne ◽  
C. Channa Reddy ◽  
Lorraine M. Sordillo
Keyword(s):  
Endothelial Cells ◽  
Mrna Expression ◽  
Adhesion Molecule ◽  
Selenium Deficiency ◽  
Neutrophil Adherence
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