Acid Phosphatases of Budding Yeast as a Model of Choice for Transcription Regulation Research

Acid phosphatases of budding yeast have been studied for more than forty years. This paper covers biochemical characteristics of acid phosphatases and different aspects in expression regulation of eukaryotic genes, which were researched using acid phosphatases model. A special focus is devoted to cyclin-dependent kinase Pho85p, a negative transcriptional regulator, and its role in maintaining mitochondrial genome stability and to pleiotropic effects of pho85 mutations.

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DNA Precursor Metabolism and Mitochondrial Genome Stability

10.21236/ada460347 ◽

2003 ◽

Author(s):

Christopher K. Mathews

Keyword(s):

Mitochondrial Genome ◽

Genome Stability ◽

Mitochondrial Genome Stability

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The influence of mitochondrial dynamics on mitochondrial genome stability

Current Genetics ◽

10.1007/s00294-017-0717-4 ◽

2017 ◽

Vol 64 (1) ◽

pp. 199-214 ◽

Cited By ~ 9

Author(s):

Christopher T. Prevost ◽

Nicole Peris ◽

Christina Seger ◽

Deanna R. Pedeville ◽

Kathryn Wershing ◽

...

Keyword(s):

Mitochondrial Genome ◽

Genome Stability ◽

Mitochondrial Dynamics ◽

Mitochondrial Genome Stability

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MMS19 localizes to mitochondria and protects the mitochondrial genome from oxidative damage

Biochemistry and Cell Biology ◽

10.1139/bcb-2017-0149 ◽

2018 ◽

Vol 96 (1) ◽

pp. 44-49 ◽

Cited By ~ 2

Author(s):

Rui Wu ◽

Qunsong Tan ◽

Kaifeng Niu ◽

Yuqi Zhu ◽

Di Wei ◽

...

Keyword(s):

Mitochondrial Genome ◽

Oxidative Damage ◽

Genome Stability ◽

Excision Repair ◽

Mass Spectrometry Analysis ◽

Mtdna Copy Number ◽

Repair Capacity ◽

Spectrometry Analysis ◽

Mitochondrial Genome Stability ◽

Mtdna Repair

MMS19 localizes to the cytoplasmic and nuclear compartments involved in transcription and nucleotide excision repair (NER). However, whether MMS19 localizes to mitochondria, where it plays a role in maintaining mitochondrial genome stability, remains unknown. In this study, we provide the first evidence that MMS19 is localized in the inner membrane of mitochondria and participates in mtDNA oxidative damage repair. MMS19 knockdown led to mitochondrial dysfunctions including decreased mtDNA copy number, diminished mtDNA repair capacity, and elevated levels of mtDNA common deletion after oxidative stress. Immunoprecipitation – mass spectrometry analysis identified that MMS19 interacts with ANT2, a protein associated with mitochondrial ATP metabolism. ANT2 knockdown also resulted in a decreased mtDNA repair capacity after oxidative damage. Our findings suggest that MMS19 plays an essential role in maintaining mitochondrial genome stability.

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Mitochondrial DNA Integrity: Role in Health and Disease

Cells ◽

10.3390/cells8020100 ◽

2019 ◽

Vol 8 (2) ◽

pp. 100 ◽

Cited By ~ 26

Author(s):

Priyanka Sharma ◽

Harini Sampath

Keyword(s):

Mitochondrial Dna ◽

Mitochondrial Genome ◽

Genome Stability ◽

Dna Lesions ◽

Genome Integrity ◽

Dna Integrity ◽

Age Related ◽

Genome Damage ◽

Health And Disease ◽

Mitochondrial Genome Stability

As the primary cellular location for respiration and energy production, mitochondria serve in a critical capacity to the cell. Yet, by virtue of this very function of respiration, mitochondria are subject to constant oxidative stress that can damage one of the unique features of this organelle, its distinct genome. Damage to mitochondrial DNA (mtDNA) and loss of mitochondrial genome integrity is increasingly understood to play a role in the development of both severe early-onset maladies and chronic age-related diseases. In this article, we review the processes by which mtDNA integrity is maintained, with an emphasis on the repair of oxidative DNA lesions, and the cellular consequences of diminished mitochondrial genome stability.

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