scholarly journals First PCR isolation of Crithidia mellificae (Euglenozoa: Trypanosomatidae) in Apis mellifera (Hymenoptera: Apidae) in Italy

2015 ◽  
Vol 47 (1) ◽  
pp. 45 ◽  
Author(s):  
Antonella Cersini ◽  
Valeria Antognetti ◽  
Raffaella Conti ◽  
Fabiana Velletrani ◽  
Giovanni Formato
Keyword(s):  
Apis Mellifera ◽  
Cytochrome B ◽  
Ribosomal Rna ◽  
Short Communication ◽  
Small Subunit ◽  
Mitochondrial Cytochrome ◽  
Small Subunit Ribosomal Rna ◽  
Mitochondrial Cytochrome B ◽  
Pcr Products

<em>Crithidia mellificae</em> (Langridge &amp; McGhee, 1967) is a trypanosomatid described in <em>Apis</em> <em>mellifera</em> (Linnaeus, 1758). The pathological effects of this parasite on the host are not well known. In this short communication, we report the first isolation of this pathogen in Italy, as realized in December 2013. The detection of <em>Crithidia</em> spp. was obtained by applying two PCR protocols that target the sequence of the mitochondrial cytochrome b (<em>Cytb</em>) and the sequence of small subunit ribosomal RNA (<em>SSUrRNA</em>), respectively. The PCR products were subjected to sequencing, which confirmed that the strain belonged to <em>Crithidia mellificae</em>.

Mitochondrial Cytochrome b DNA Sequence Variations: An Approach to Fish Species Identification in Processed Fish Products

2005 ◽  
Vol 68 (2) ◽  
pp. 421-425 ◽  
Author(s):  
TIZIANA PEPE ◽  
MICHELE TROTTA ◽  
ISOLINA DI MARCO ◽  
PAOLA CENNAMO ◽  
ANIELLO ANASTASIO ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Cytochrome B ◽  
Fish Species ◽  
Mitochondrial Cytochrome ◽  
Fish Products ◽  
Community Regulation ◽  
Sequence Variations ◽  
Fish Species Identification ◽  
Mitochondrial Cytochrome B ◽  
Pcr Products

The identification of fish species in food products is problematic because morphological features of the fish are partially or completely lost during processing. It is important to determine fish origin because of the increasing international seafood trade and because European Community Regulation 104/2000 requires that the products be labeled correctly. Sequence analysis of PCR products from a conserved region of the cytochrome b gene was used to identity fish species belonging to the families Gadidae and Merluccidae in 18 different processed fish products. This method allowed the identification of fish species in all samples. Fish in all of the examined products belonged to these two families, with the exception of one sample of smoked baccalà (salt cod), which was not included in the Gadidae cluster.

PHYLOGENY OF DINOFLAGELLATES BASED ON MITOCHONDRIAL CYTOCHROME B AND NUCLEAR SMALL SUBUNIT RDNA SEQUENCE COMPARISONS1

2005 ◽  
Vol 41 (2) ◽  
pp. 411-420 ◽  
Author(s):  
Huan Zhang ◽  
Debashish Bhattacharya ◽  
Senjie Lin
Keyword(s):  
Cytochrome B ◽  
Small Subunit ◽  
Rdna Sequence ◽  
Mitochondrial Cytochrome ◽  
Small Subunit Rdna ◽  
Mitochondrial Cytochrome B

scholarly journals A discontinuous small subunit ribosomal RNA in Tetrahymena pyriformis mitochondria.

1986 ◽  
Vol 261 (11) ◽  
pp. 5187-5193
Author(s):  
M N Schnare ◽  
T Y Heinonen ◽  
P G Young ◽  
M W Gray
Keyword(s):  
Ribosomal Rna ◽  
Tetrahymena Pyriformis ◽  
Small Subunit ◽  
Small Subunit Ribosomal Rna

scholarly journals Nuclear small-subunit ribosomal RNA gene sequences from representative Ceramiaceae (Ceramiales, Rhodophyta)

1996 ◽  
Vol 31 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Gary W. Saunders ◽  
Isabelle M. Strachan ◽  
John A. West ◽  
Gerald T. Kraft
Keyword(s):  
Ribosomal Rna ◽  
Small Subunit ◽  
Gene Sequences ◽  
Ribosomal Rna Gene ◽  
Small Subunit Ribosomal Rna
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