scholarly journals Prospective nucleotide sequence analysis of methicillin resistant Staphylococcus aureus isolates from Sokoto state

2020 ◽  
Vol 35 (1) ◽  
Author(s):  
Shuaibu Suleiman Adeiza ◽  
Josiah Ademola Onaolapo ◽  
Busayo Olalekan Olayinka
Keyword(s):  
Staphylococcus Aureus ◽  
Phylogenetic Analysis ◽  
Nucleotide Sequence ◽  
Molecular Characterization ◽  
Nucleotide Sequence Analysis ◽  
Local Data ◽  
The Past ◽  
Mrsa Strains ◽  
Hospital Acquired

The epidemiology of MRSA has evolved considerably. In the past, MRSA strains could be traced back to a hospital or clinic source, but over the years there appear to be a decrease in hospital acquired MRSA and an upsurge in the occurrence in community acquired strains. Even-though in Nigeria there are studies that have reported on the molecular characterization of MRSA, Sadly, not much effort has been put into analysis and submission of sequenced data to Gene repositories. Based on information available to us, this is the first study to have successfully deposited nucleotide sequences of MRSA strains from Sokoto state owned Hospital into the Genbank database. Sequence reads of Molecular characterization should be deposited into Gene repositories database by researchers to further populate the database and improve the efficiency of local phylogenetic analysis to foster better bioinformatics as paucity of local data exists in these databases.

scholarly journals Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant

2017 ◽  
Vol 30 (1) ◽  
pp. 172-174 ◽  
Author(s):  
Hiroya Ito ◽  
Sayaka Takahashi ◽  
Tetsuo Asai ◽  
Yutaka Tamura ◽  
Koshi Yamamoto
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Gene Cluster ◽  
Molecular Characterization ◽  
Dna Sequence ◽  
Actinobacillus Pleuropneumoniae ◽  
Nucleotide Sequence Analysis ◽  
Urease Gene ◽  
Negative Mutant

An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates.

scholarly journals Complete nucleotide sequence and molecular characterization of two lytic Staphylococcus aureus phages: 44AHJD and P68

2003 ◽  
Vol 219 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Dietmar Vybiral ◽  
Marian Takáč ◽  
Martin Loessner ◽  
Angela Witte ◽  
Uwe Ahsen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Nucleotide Sequence ◽  
Molecular Characterization ◽  
Complete Nucleotide Sequence

scholarly journals Molecular Characterization of Community-Associated Methicillin-Resistant Staphylococcus aureus in Iranian Burn Patients

2019 ◽  
Vol 14 (4) ◽  
pp. 284-289 ◽  
Author(s):  
Samira Tajik ◽  
Shahin Najar Peerayeh ◽  
Bita Bakhshi ◽  
Reza Golmohammadi
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Significant Proportion ◽  
Burn Patients ◽  
Burn Wound ◽  
Methicillin Resistant ◽  
Pfge Typing ◽  
Mrsa Strains

Background & Objective: Methicillin-resistant Staphylococcus aureus (MRSA) is reported as one of the important bacterial causes of burn wound infections. This study was carried out to investigate molecular characterization of community-associated MRSA (CA-MRSA) isolated from Iranian burn patients. Methods: A total of 31 isolates of S. aureus were collected fromthe Motahari Burns Hospital (Tehran, Iran) in 2016. All isolates were collected from outpatients and inpatients within 48 hours of admission. The mecA, pvl, tsst-1, hla-α, and psmα genes detecting, SCCmec, agr and PFGE typing were done. Results: A total of 13 (41.9%) isolates were cefoxitin-resistant and mecA-positive, which were considered as MRSA. The SCCmec typing MRSA strains revealed type II in 1 (7.7%), type III in 9 (69.2%), and other types in 3 isolates (23.7%) cases. The agrtyping of all 31 isolates showed that 14 (45.2%), 1 (3.2%), 6 (19.4%), and 10 (32.3%) strains belonged to agr groups 1, 3, 4, and unknown type, respectively. The pvl, tsst-1, hla-α, and psmα genes were positive in 3 (9.7%), 4 (12.9%), 21 (67.7%), and 31 (100%) isolates, respectively. Considering the cut-off values of ≥50%, 3 groups of related isolates (cluster A1, B1, and C1) in PFGE study were observed. Conclusion: The MRSA strains of this study were initially isolated as Community-associated S. aureus (CA-MRSA); however molecular characterization showed that a significant proportion of them had hospital-associated MRSA (HA-MRSA) features. Therefore, it is likely that the HA-MRSA strains are spread among the community.

scholarly journals Isolation, Detection and Molecular Characterization of Staphylococcus aureus from Postoperative Infections

2020 ◽  
pp. 63-67
Author(s):  
Ritya Mary Jibu ◽  
R. V. Geetha ◽  
T. Lakshmi
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Infection ◽  
Molecular Characterization ◽  
Methicillin Resistance ◽  
Healthcare Associated Infection ◽  
Postoperative Infections ◽  
Coagulase Negative Staphylococci ◽  
Mrsa Strains ◽  
Healthcare Associated

Post operative infections that occur after surgical procedures can cause a lot of complications like sepsis, organ failure or even death. These are the third most commonly reported healthcare associated infection. The most common cause of wound infection regardless of procedure performed remains gram-positive cocci which comprise more than 50% of all infections. Specifically, Staphylococcus aureus and coagulase-negative staphylococci are the most frequent organisms isolated from a wound infection. There has been an increasing incidence of MRSA strains reported in hospitals across the globe. The main aim of our study is isolation, detection and molecular characterization of Staphylococcus aureus from postoperative infections.  Samples were collected from post operative patients with infected wounds. The area around the wound was cleaned. Exudates were collected from the wound with a sterile swab stick. The samples were inoculated on different solid culture mediums and the plates were incubated in the presence of oxygen at 37°C overnight. There were many standard procedures done in which tube coagulase was taken as the main criteria. Antibiotic susceptibility testing was done by Kirby Bauer method following Clinical and Laboratory Standards Institute (CLSI) guidelines using commercially available cefoxitin (30 μg) disc (HiMedia) and the results were compared with Staphylococcus aureus ATCC 25923 and MRSA ATCC 43300 control strains. The MRSA strains were identified and detection of Mec A gene that codes for methicillin resistance is done using PCR technique.

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