scholarly journals Molecular characterisation of infectious bursal disease virus in Namibia, 2017

2019 ◽  
Vol 86 (1) ◽  
Author(s):  
Umberto Molini ◽  
Gottlieb Aikukutu ◽  
Juliet Kabajani ◽  
Siegfried Khaiseb ◽  
Giovanni Cattoli ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Partial Sequence ◽  
Molecular Characterisation ◽  
Rt Pcr ◽  
Bursal Disease ◽  
Control And Management

Between July and September 2017, samples collected from six unvaccinated chickens in Namibia were shown to be positive for infectious bursal disease virus (IBDV) by RT-PCR. Partial sequence and phylogenetic analysis of the VP1 and VP2 genes from six viruses revealed that they all belong to the very virulent pathotype (Genogroup 3) and are genetically very similar to IBDVs identified in neighbouring Zambia. This is the first molecular characterisation of IBDV in Namibia and has implications on the control and management of the disease in the country.

A Practical Tissue Sampling Method Using Ordinary Paper for Molecular Detection of Infectious Bursal Disease Virus RNA by RT-PCR

2006 ◽  
Vol 50 (4) ◽  
pp. 556-560 ◽  
Author(s):  
Min Thein Maw ◽  
Tsuyoshi Yamaguchi ◽  
Christopher J. Kasanga ◽  
Kaori Terasaki ◽  
Hideto Fukushi
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Sampling Method ◽  
Molecular Detection ◽  
Infectious Bursal Disease ◽  
Tissue Sampling ◽  
Rt Pcr ◽  
Virus Rna ◽  
Bursal Disease

scholarly journals Effective inhibition of infectious bursal disease virus replication by recombinant avian adeno-associated virus-delivered microRNAs

2009 ◽  
Vol 90 (6) ◽  
pp. 1417-1422 ◽  
Author(s):  
Yongjuan Wang ◽  
Huaichang Sun ◽  
Pengpeng Shen ◽  
Xinyu Zhang ◽  
Xiaoli Xia
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Viral Vector ◽  
Infectious Bursal Disease ◽  
Inhibitory Effects ◽  
Rt Pcr ◽  
Adeno Associated Virus ◽  
Rnai Technology ◽  
Bursal Disease ◽  
In Cells

RNA interference (RNAi) is a novel antiviral strategy against a variety of virus infections. Infectious bursal disease virus (IBDV) causes an economically important disease in young chickens. This study demonstrated efficient inhibition of IBDV replication by recombinant avian adeno-associated virus (rAAAV)-delivered anti-VP1 and anti-VP2 microRNAs (miRNAs). In the viral vector-transduced cells, sequence-specific miRNA expression was detected by poly(A)-tailed RT-PCR. Reporter assays using a pVP2-EGFP vector showed significant and long-lasting inhibition of VP2–EGFP expression in cells transduced with anti-VP2 miRNA-expressing rAAAV-RFPmiVP2E, but not with the control miRNA-expressing rAAAV-RFPmiVP2con or anti-VP1 miRNA-expressing rAAAV-RFPmiVP1. Semi-quantitative RT-PCR and/or virus titration assays showed a significant inhibitory effect on homologous IBDV replication in cells transduced with rAAAV-RFPmiVP1 or rAAAV-RFPmiVP2E. For two heterologous IBDV isolates, transduction with rAAAV-RFPmiVP1 led to slightly weaker but similar inhibitory effects, whereas transduction with rAAAV-RFPmiVP2E resulted in significantly weaker and different inhibitory effects. These results suggest that rAAAV could act as an efficient vector for miRNA delivery into avian cells and that VP1 is the more suitable target for interfering with IBDV replication using RNAi technology.

scholarly journals Phylogenetic Analysis Reveals a Correlation between the Expansion of Very Virulent Infectious Bursal Disease Virus and Reassortment of Its Genome Segment B

2006 ◽  
Vol 80 (17) ◽  
pp. 8503-8509 ◽  
Author(s):  
Chung-Chau Hon ◽  
Tsan-Yuk Lam ◽  
Alexei Drummond ◽  
Andrew Rambaut ◽  
Yiu-Fai Lee ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Population Dynamics ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Genome Segment ◽  
Infectious Bursal Disease ◽  
Reassortment Event ◽  
The Past ◽  
Bursal Disease ◽  
Past Population

ABSTRACT Infectious bursal disease virus (IBDV) is a birnavirus causing immunosuppressive disease in chickens. Emergence of the very virulent form of IBDV (vvIBDV) in the late 1980s dramatically changed the epidemiology of the disease. In this study, we investigated the phylogenetic origins of its genome segments and estimated the time of emergence of their most recent common ancestors. Moreover, with recently developed coalescence techniques, we reconstructed the past population dynamics of vvIBDV and timed the onset of its expansion to the late 1980s. Our analysis suggests that genome segment A of vvIBDV emerged at least 20 years before its expansion, which argues against the hypothesis that mutation of genome segment A is the major contributing factor in the emergence and expansion of vvIBDV. Alternatively, the phylogeny of genome segment B suggests a possible reassortment event estimated to have taken place around the mid-1980s, which seems to coincide with its expansion within approximately 5 years. We therefore hypothesize that the reassortment of genome segment B initiated vvIBDV expansion in the late 1980s, possibly by enhancing the virulence of the virus synergistically with its existing genome segment A. This report reveals the possible mechanisms leading to the emergence and expansion of vvIBDV, which would certainly provide insights into the scope of surveillance and prevention efforts regarding the disease.

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