scholarly journals Efficacy of an in-house polymerase chain reaction assay for rapid diagnosis of Mycobacterium tuberculosis in patients with tubercular lymphadenitis: Comparison with fine needle aspiration cytology and conventional techniques

2010 ◽  
Vol 53 (4) ◽  
pp. 714 ◽  
Author(s):  
Sunil Sethi ◽  
AshwiniKumar Mishra ◽  
ShivSekhar Chatterjee ◽  
Ajay Wanchu ◽  
Raje Nijhawan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Needle Aspiration ◽  
Rapid Diagnosis ◽  
Chain Reaction ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Polymerase Chain ◽  
Needle Aspiration Cytology ◽  
Tubercular Lymphadenitis

scholarly journals Problems and Solution to Diagnose Extrapulmonary Tuberculosis in Central Region of Nepal

Med Phoenix ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 41-43
Author(s):  
Ravi Shankar Gupta ◽  
Tarannum Khatun ◽  
Akhtar Alam Ansari ◽  
Amrullah Shidiki ◽  
Dipak Bhargava ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Tuberculosis ◽  
Correct Diagnosis ◽  
Extrapulmonary Tuberculosis ◽  
Needle Aspiration ◽  
Retropharyngeal Abscess ◽  
Chain Reaction ◽  
Specificity And Sensitivity ◽  
Polymerase Chain ◽  
Needle Aspiration Cytology

Extrapulmonary Tuberculosis is high, challenging the clinicians to make correct diagnosis. Microscopy, culture and fine needle aspiration cytology have their limitations in regard to specificity and sensitivity. In this report, polymerase chain reaction is used for detecting and distinguishing Extrapulmonary Tuberculosis. A case of retropharyngeal abscess was selected from which pus was collected which was negative for microscopy and culture in routine microbiology as well as mycobacteriology. Cytopathological examination was also negative. Polymerase chain reaction was applied to detect Mycobacterium tuberculosis specific IS6110 gene. The patients responded with anti-tuberculosis treatment well. Polymerase chain reaction was introduced for diagnosis of Extrapulmonary Tuberculosis since it can be done within hours, monitor therapy and also differentiate Mycobacterium tuberculosis from other Mycobacterial species.MED Phoenix Volume (1), Issue (1) July 2016, page: 41-43

scholarly journals Tuberculous mycobacteria bacilli fluorescence and compare with Ziehl- Neelsen stain in fine-needle aspiration cytology of tubercular lymphnode

2016 ◽  
Vol 2 (2) ◽  
pp. 66
Author(s):  
Mani Krishna ◽  
Adesh Kumar
Keyword(s):  
Infectious Disease ◽  
Mycobacterium Tuberculosis ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Needle Aspiration ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Fluorescent Method ◽  
Fluorescent Stain ◽  
Needle Aspiration Cytology

<p class="abstract"><strong>Background:</strong> Tuberculosis is infectious disease caused by <em>Mycobacterium tuberculosis</em>. There are various methods for diagnosis of tuberculosis such as direct clinical material examination of tubercular bacilli by Ziehl – Neelsen (ZN) staining, demonstration of tubercular bacilli by auramine – rhodamine (AR) staining and autofluorescence (AF).</p><p class="abstract"><strong>Methods:</strong> Present study was done clinically suspected tubercular patients. All received samples Zn stain, fluorescent stain and PAP stain were applied.</p><p class="abstract"><strong>Results:</strong> Among the clinically suspected patients 88 was diagnosed with tuberculosis. Female preponderance was noted accounting for 60.23% (53/88) of cases. Of the 88 aspirates, the smear positivity for acid fast bacilli (AFB) on the ZN method was 37.5% (33/88) while the positivity increased to 81.82% (72/88) on the AR fluorescent method and 86.36% (76/88) on AF.</p><strong>Conclusions:</strong> AF staining is more sensitive than the auramine – rhodamine fluorescent and ZN staining in demonstration of mycobacterium bacilli in fine needle aspiration cytology of tubercular lymphnode.

scholarly journals Pattern of Lymphadenopathy on Fine Needle Aspiration Cytology in a Tertiary Level Hospital in Kathmandu

2018 ◽  
Vol 1 (2) ◽  
pp. 97-99
Author(s):  
Amar Narayan Shrestha ◽  
Shova Kunwar ◽  
Barun Babu Aryal ◽  
Anurag Adhikari ◽  
Ayusha Poudel
Keyword(s):  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Needle Aspiration ◽  
Tertiary Level ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Granulomatous Lymphadenitis ◽  
Needle Aspiration Cytology ◽  
Tubercular Lymphadenitis ◽  
Level Hospital

Introduction: Fine Needle Aspiration Cytology is a relatively simple, inexpensive and rapid diagnostic procedure for identifying cause of lymphadenopathy without need for surgical procedures. This study aims to explain the pattern of lymphadenopathy seen on fine needle aspiration cytology in a tertiary level hospital in KathmanduMaterials and Methods: This study was conducted at Shree Birendra Hospital Nepal. Cases of lymph node FNAC done in the years 2073 and 2074 BS were included in the study. The cases were classified into reactive lymphadenitis, granulomatous lymphadenitis, tubercular lymphadenitis, lymphomas, leukemias, and metastases.Results: A total of 215 patients were included in the study, ranging in age from 2 to 84 years, out of which 98 were female and 117 were male. Reactive lymphadenitis was the most common diagnosis (n=126; 58.6%), followed by granulomatous lymphadenitis (n=34; 15.8%) and tubercular lymphadenitis (n=18; 8.4%). There were 18 cases of metastatic malignancies, 15 cases of suppurative lymphadenitis, and 2 cases of Hodgkin lymphoma and 2 cases of non-Hodgkin lymphoma.Conclusions: Reactive lymphadenitis is the most common type of lymphadenopathy encountered in FNAC, while in the elderly, metastases are more common.

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