Expression of P53 protein and Ki-67 antigen in oral leukoplakia with different histopathological grades of epithelial dysplasia

OBJECTIVES: (1) To determine whether the protein of the suppressor gene p53 accumulates in leukoplakia of the oral cavity in individuals who use snuff; and (2) to determine whether a correlation exists between the accumulation of p53 protein and the degree of epithelial dysplasia present in oral leukoplakia. DESIGN: Retrospective analysis of archival tissue specimens. SETTING: The University Hospital, a tertiary referral hospital affiliated with the Oklahoma University Medical Center, Oklahoma City, Oklahoma. PATIENTS: In the first part of the study, biopsy specimens of leukoplakia from 12 persons who used snuff were compared with specimens from uninvolved oral mucosa of the same persons and with biopsy specimens from 12 nontobacco-using persons. In the second part of the study, accumulation of p53 protein was determined in 42 archival paraffin-embedded specimens from oral leukoplakia and correlated with the degree of epithelial dysplasia. METHODS: Accumulation of p53 protein was assessed by immunoperoxidase staining with four different primary antibodies. Positive cells were counted in five consecutive high-power fields. RESULTS: In part one, the average number of positive cells in the leukoplakia of snuff-users (21.89 ± 4.33; mean ± SE) was higher than that of normal-appearing mucosa (4.00 ± 1.0; p < 0.05) and that of nontobacco-using controls (7.00 ± 5.04). In part two, the average number of positive cells was higher in the moderately dysplastic (140.36 ± 30.03) and severely dysplastic lesions (232.86 ± 26.85) than in the mildly dysplastic lesions (14.53 ± 3.33; p < 0.05). The correlation between the degree of epithelial dysplasia and the number of cells positive is strong (Spearman's correlation coefficient = 0.853). CONCLUSIONS: The accumulation of p53 protein in leukoplakia of snuff-users is higher than in normal-appearing oral mucosa from both snuff-users and nontobacco-using controls. A strong correlation exists between the degree of epithelial dysplasia present in oral leukoplakia and the number of cells staining positive for p53. The accumulation of p53 protein holds potential as an intermediate end point in studies of chemoprevention of oral cancer.

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Expression of p53, bcl-2, and ki-67 Proteins in the Inﬂammatory Regenerative and Dysplastic Epithelial Lesions of Flat Colonic Mucosa

Bosnian Journal of Basic Medical Sciences ◽

10.17305/bjbms.2006.3208 ◽

2008 ◽

Vol 6 (1) ◽

pp. 39-45 ◽

Cited By ~ 3

Author(s):

Svjetlana Radović ◽

Zora Vukobrat-Bijedić ◽

Ivan Selak ◽

Mirsad Babić

Keyword(s):

Cellular Proliferation ◽

P53 Protein ◽

Normal Mucosa ◽

Labelling Index ◽

Epithelial Dysplasia ◽

Severe Dysplasia ◽

Ki 67 ◽

Mild Dysplasia ◽

Sporadic Cases ◽

Epithelial Lesions

The aim of the study was to define the distribution of p53, bcl-2 and Ki-67 proteins in the inflammatory-regenerative and dysplastic lesionsof the colon mucosa. The relationship between the presentation of p53, bcl-2 and Ki-67 proteins and the intensity of the inflammatory-regenerative and dysplastic lesions in the colon flat mucosa was investigated as well. Biopsy specimens from 270 patients were examined: 74 were classified as inflammatory-regenerative and 196 as dysplastic lesions (108 mild, 58 moderate, and 30 severe dysplasia). The expression of all three proteins was assessed on the basis of location, quantity, and intensity of immunostaining, by counting antigen positive cells, in comparison with normal mucosa and adenocarcinoma. p53 protein appears only in sporadic cases (6.6%) of severe dysplasia. Bcl-2 expression appears significantly (p<0.005) more often in cases of mild dysplasia (61.1%) compared to inflammatory-regenerative mucosa (14.8%). In cases of mild dysplasia, bcl-2 positive cells were spreading from the lower third to the middle third of the crypts. Bcl-2 expression was maintained through the stadiums of moderate and severe dysplasia (75.8%), where antigen positive cells were found all along the crypts. A significant increase (p<0.005) in the expression of nuclear protein Ki-67 was noticed in the stadiums of moderate (labelling index =26.3) compared to mild dysplasia (labelling index=16.7), and severe (labelling index=36.7) compared to moderate dysplasia, where the zone of cellular proliferation was widen along the whole crypt length. In the process of the development of epithelial dysplasia in the flat mucosa of colon a degree of the gene p53 alteration is low and appears only in sporadic cases of severe dysplasia. Mutation of the bcl-2 gene is involved in the genesis of the lesion but not in its progression to carcinoma. Increased expressionof Ki-67 protein speaks in favour of an increased cellular proliferation which, together with the above mentioned mechanisms, is involved in the process of occurrence and progression of epithelial dysplasia in the flat mucosa of colon.

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Numerical Chromosomal Aberrations in Borderline, Benign, and Malignant Epithelial Tumors of the Ovary: Correlation With p53 Protein Overexpression and Ki-67

Journal of the Society for Gynecologic Investigation ◽

10.1016/s1071-5576(97)00061-0 ◽

1997 ◽

Vol 4 (5) ◽

pp. 262-264 ◽

Cited By ~ 7

Author(s):

P Kohlberger

Keyword(s):

Chromosomal Aberrations ◽

P53 Protein ◽

Protein Overexpression ◽

Ki 67 ◽

Epithelial Tumors

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The Role of Immunohistochemical Markers for the Diagnosis and Prognosis of Adrenocortical Neoplasms

Journal of Personalized Medicine ◽

10.3390/jpm11030208 ◽

2021 ◽

Vol 11 (3) ◽

pp. 208

Author(s):

Anna Angelousi ◽

Georgios Kyriakopoulos ◽

Fani Athanasouli ◽

Anastasia Dimitriadi ◽

Eva Kassi ◽

...

Keyword(s):

Transcriptome Analysis ◽

Cox Regression ◽

P53 Protein ◽

Immunohistochemical Analysis ◽

Ki 67 ◽

Diagnosis And Prognosis ◽

Immunohistochemical Markers ◽

Small Series ◽

Morphological Criteria ◽

Survival And Development

Adrenal cortical carcinoma (ACC) is a rare cancer with poor prognosis that needs to be distinguished from adrenocortical adenomas (ACAs). Although, the recently developed transcriptome analysis seems to be a reliable tool for the differential diagnosis of adrenocortical neoplasms, it is not widely available in clinical practice. We aim to evaluate histological and immunohistochemical markers for the distinction of ACCs from ACAs along with assessing their prognostic role. Clinical data were retrospectively analyzed from 37 patients; 24 archived, formalin-fixed, and paraffin-embedded ACC samples underwent histochemical analysis of reticulin and immunohistochemical analysis of p27, p53, Ki-67 markers and were compared with 13 ACA samples. Weiss and Helsinki scores were also considered. Kaplan−Meier and univariate Cox regression methods were implemented to identify prognostic effects. Altered reticulin pattern, Ki-67% labelling index and overexpression of p53 protein were found to be useful histopathological markers for distinguishing ACAs from ACCs. Among the studied markers, only pathological p53 nuclear protein expression was found to reach statistically significant association with poor survival and development of metastases, although in a small series of patients. In conclusion, altered reticulin pattern and p53/Ki-67 expression are useful markers for distinguishing ACCs from ACAs. Immunohistopathology alone cannot discriminate ACCs with different prognosis and it should be combined with morphological criteria and transcriptome analysis.

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Immunohistochemical study of p53 expression in endometrial carcinomas: correlation with markers of proliferating cells and clinicopathologic features

International Journal of Gynecological Cancer ◽

10.1046/j.1525-1438.1993.03060363.x ◽

1993 ◽

Vol 3 (6) ◽

pp. 363-368 ◽

Cited By ~ 10

Author(s):

T. Hachisuga ◽

K. Fukuda ◽

M. Uchiyama ◽

N. Matsuo ◽

T. Iwasaka ◽

...

Keyword(s):

Dna Polymerase ◽

Proliferative Activity ◽

P53 Protein ◽

Myometrial Invasion ◽

Proliferating Cells ◽

Ki 67 ◽

Normal Endometrium ◽

P53 Expression ◽

Dna Polymerase Α ◽

Endometrial Carcinomas

Using anti-p53 (PAb1801 and PAb240), anti-DNA polymerase α and Ki-67 monoclonal antibodies, the expression of p53 was studied in 11 normal endometria, 14 endometrial hyperplasias and 27 endometrial carcinomas and its relationship to the proliferative activity of the tumors was examined. Normal endometria and simple hyperplasias were completely negative for p53. The PAb1801 indices of complex hyperplasias and complex atypical hyperplasias were 2.5±1.8% and 5.0±3.2%, respectively. The PAb1801 indices of grade 1, grade 2 and grade 3 endometrial carcinomas were 10.2±14.2%, 44.4±29/0% and 45.0±32.5%, respectively. These results indicate a progressively enhanced p53 expression in the sequence from normal endometrium, through hyperplasia to carcinoma. A significant correlation between p53 expression and labeling indices of Ki-67 and DNA polymerase α was observed in endometrial carcinomas. The endo-metrial carcinomas with p53 overexpression developed mainly in post-menopausal patients and were frequently high-grade tumors with deep myometrial invasion. These findings may indicate that overexpression of p53 protein contributes to the proliferative activity of the tumor cells.

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Expression of p53 Protein and Ki-67 Antigen in Gingival Hyperplasia Induced by Nifedipine and Phenytoin

Journal of Periodontology ◽

10.1902/jop.1999.70.6.581 ◽

1999 ◽

Vol 70 (6) ◽

pp. 581-586 ◽

Cited By ~ 29

Author(s):

Keiichi Saito ◽

Shiro Mori ◽

Naoko Tanda ◽

Seizaburo Sakamoto

Keyword(s):

P53 Protein ◽

Gingival Hyperplasia ◽

Ki 67

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