Antimicrobial efficacy of nanosilver and chitosan against Streptococcus mutans, as an ingredient of toothpaste formulation: An in vitro study

Introduction: Dental caries is a pandemic disease caused by Streptococcus mutans. Numerous preventive strategies have been developed to prevent dental caries with modern medicines. Recently, there has been a shift from use of modern medicines to herbal ayurvedic preparations which are easily available and cause least possible side effects but have not been tested against S. mutans. Aim: To evaluate antimicrobial value of Pudina, Tulsi and Curry leaves against S. mutans by modified direct contact test. Materials and Methods: This in vitro study used finely powdered form of Pudina, Tulsi and Curry leaves extracts (Hakim Chi Chi Pharmacy, Surat, India) without any added preservatives. The test microorganism S. mutans was isolated from human saliva using mitis salivarius agar. The Minimum Inhibition Concentration (MIC) was determined using agar well diffusion for Group 1 (Tulsi), Group 2 (Pudina), Group 3 (Curry leaves) and Group 4 (Chlorhexidine). Using this MIC, modified Direct Contact Test was performed for specific evaluation of the antimicrobial efficacy of each product. Chlorhexidine (0.2%) was used as a positive control for the comparison of each product. The results of modified direct contact test were subjected to ANOVA Test and Tukey’s Test using SPSS software version 23.0 which showed that when compared against chlorhexidine, the antimicrobial efficacy against S. mutans in decreasing order was of curry leaves, tulsi and pudina and the result of the study was significant. Results: Curry leaves showed higher antimicrobial efficacy than tulsi and pudina when compared against chlorhexidine (control) and the results were statistically significant (p<0.05). Conclusion: Curry leaf extract shows promising antimicrobial property against S. mutans and can be recommended for caries control with further investigations.

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Evaluation of antimicrobial efficacy of Triphala (an Indian Ayurvedic herbal formulation) and 0.2% chlorhexidine against Streptococcus mutans biofilm formed on tooth substrate: An in vitro study

Indian Journal of Dental Research ◽

10.4103/0970-9290.142539 ◽

2014 ◽

Vol 25 (4) ◽

pp. 475 ◽

Cited By ~ 5

Author(s):

J Prabhakar ◽

S Balagopal ◽

MS Priya ◽

S Selvi ◽

M Senthilkumar

Keyword(s):

Streptococcus Mutans ◽

In Vitro Study ◽

Vitro Study ◽

Antimicrobial Efficacy ◽

Herbal Formulation

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Antimicrobial Efficacy of Nanopropolis Coated Vs Silver-Curcumin Nanoparticles Coated Gutta-Percha Points on Various Microbial Species. A Comparative In Vitro Study

Egyptian Dental Journal ◽

10.21608/edj.2020.31338.1132 ◽

2020 ◽

Vol 66 (3) ◽

pp. 1893-1902

Author(s):

Hajer Abd El Hamid ◽

Mohamed Abdel-Aziz ◽

Fatma Abu Naeem

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Antimicrobial Efficacy ◽

Curcumin Nanoparticles ◽

Microbial Species ◽

Gutta Percha

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Evaluation of Antimicrobial Efficacy and Permeability of Various Sealing Materials at the Implant–Abutment Interface—A Pilot In Vitro Study

Materials ◽

10.3390/ma14020385 ◽

2021 ◽

Vol 14 (2) ◽

pp. 385

Author(s):

Igor Smojver ◽

Marko Vuletić ◽

Dražena Gerbl ◽

Ana Budimir ◽

Mato Sušić ◽

...

Keyword(s):

Fungal Infections ◽

Aerobic Condition ◽

In Vitro Study ◽

Vitro Study ◽

Antimicrobial Efficacy ◽

Sealing Material ◽

Implant Abutment ◽

Static Conditions ◽

Sealing Materials

The microenvironment of the oral cavity is altered when an implant, a biocompatible foreign body, is inserted into the mouth. Bacteria settle in the tissues in and around the implant due to the passage of microorganisms through the microgap at the connection of the implant and prosthetic abutment. To prevent colonization of the implant by microorganisms, one idea is to use sealing and antimicrobial materials to decontaminate the implant–abutment interface and close the microgap. The purpose of this study is to evaluate the antimicrobial efficacy and permeability of different types of sealing materials at the implant–abutment interface, under static conditions. Three different sealing material (GapSeal gel, Oxysafe gel and Flow.sil) were used for sealing the implant–abutment interfaces in 60 titanium dental implants, which were first contaminated with a solution containing Staphylococcus aureus and Candida albicans for 14 days under an aerobic condition. Results showed that a complete seal against bacterial infection was not formed at the implant–abutment interface, while for fungal infections, only GapSeal material helped to prevent microleakage. Findings of this in vitro study reported that application of sealing material before abutment connection may reduce peri-implant bacterial and fungal population compared with the interface without sealing material.

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