Involvement of Neurotransmitter and Nrf2 in Nicotine- and Cigarette Smoke-Induced Testicular Toxicity in Adult Rats

The current study was aimed to investigate the ameliorative effect of lycopene against gentamicin-induced testicular toxicity in adult rat testes. Pretreatment with lycopene (4 mg/kg/day) significantly prevented the decrease in the absolute testes weight and relative testes weight and the reduction in sperm count, motility, viability, and daily sperm production in gentamicin (100 mg/kg/day)-treated rats. Gentamicin significantly decreased the level of serum testosterone and testicular lactate dehydrogenase-X and G6PDH activities but a marked increase was observed upon pretreatment with lycopene. Testicular caspase-3 and -9 activities were significantly increased but lycopene showed significant protection from gentamicin-induced apoptosis. Oxidative stress was induced by gentamicin treatment as evidenced by increased hydrogen peroxide level and lipid peroxidation and decreased the antioxidant enzymes superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activities and glutathione content. These alterations were effectively prevented by lycopene pretreatment. Histopathological examination showed loss of spermatogenesis and morphological abnormalities of the testis after treatment with gentamycin. These abnormalities were effectively normalized by pretreatment with lycopene. In conclusion, gentamicin decreases rat testes weight and inhibits spermatogenesis. It induces oxidative stress and apoptosis by possible mitochondrial dysfunction. These data provide insight into the mode of action of gentamicin-induced testicular toxicity and the beneficial role provided by lycopene to restore the suppressed spermatogenesis.

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The effect of coadministration of α-tocopherol and ascorbic acid on arsenic trioxide-induced testicular toxicity in adult rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2012-0039 ◽

2013 ◽

Vol 24 (4) ◽

Cited By ~ 18

Author(s):

Prabir Kumar Mukhopadhyay ◽

Avijit Dey ◽

Sanjit Mukherjee ◽

Nirmal Kumar Pradhan

Keyword(s):

Ascorbic Acid ◽

Arsenic Trioxide ◽

Testicular Toxicity ◽

Adult Rats

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Perinatal exposure to 2,2′,4′4′ −Tetrabromodiphenyl ether induces testicular toxicity in adult rats

Toxicology ◽

10.1016/j.tox.2017.07.006 ◽

2017 ◽

Vol 389 ◽

pp. 21-30 ◽

Cited By ~ 19

Author(s):

Ahmed Khalil ◽

Mikhail Parker ◽

Sarah E. Brown ◽

Sebnem E. Cevik ◽

Lian W. Guo ◽

...

Keyword(s):

Perinatal Exposure ◽

Testicular Toxicity ◽

Adult Rats

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Lipoic acid andCalligonum comosumonattenuate aroclor 1260-induced testicular toxicity in adult rats

Environmental Toxicology ◽

10.1002/tox.22310 ◽

2016 ◽

Vol 32 (4) ◽

pp. 1147-1157 ◽

Cited By ~ 3

Author(s):

Hamdy A. A. Aly ◽

Abdulrahman M. Alahdal ◽

Ayman A. Nagy ◽

Hossam M. Abdallah ◽

Essam A. Abdel-Sattar ◽

...

Keyword(s):

Lipoic Acid ◽

Testicular Toxicity ◽

Adult Rats

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P09: Susceptibilities to acrylamide-induced neurotoxicity and testicular toxicity between young and adult rats

Experimental and Toxicologic Pathology ◽

10.1016/j.etp.2009.02.095 ◽

2009 ◽

Vol 61 (4) ◽

pp. 406

Author(s):

Miwa Takahashi ◽

Kaoru Inoue ◽

Midori Yoshida ◽

Makoto Shibutani ◽

Akiyoshi Nishikawa

Keyword(s):

Testicular Toxicity ◽

Adult Rats

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Perinatal sidestream cigarette smoke exposure and the developing pulmonary surfactant system in rats

Human & Experimental Toxicology ◽

10.1191/096032799678839923 ◽

1999 ◽

Vol 18 (4) ◽

pp. 206-211 ◽

Cited By ~ 9

Author(s):

Sriram Subramaniaml ◽

Suseela Srinivasan ◽

Paul M Bummer ◽

C Gary Gairola

Keyword(s):

Cigarette Smoke ◽

Surface Activity ◽

Pulmonary Surfactant ◽

Whole Body ◽

Cigarette Smoke Exposure ◽

Perinatal Exposure ◽

Sprague Dawley ◽

Adult Rats ◽

Surfactant System ◽

Sidestream Smoke

1 Epidemiological studies have strongly implicated passive smoking with increased incidence of various respiratory diseases in children. Our earlier studies have shown that chronic exposure to tobacco smoke significantly changes the composition and the surface activity of the pulmonary surfactant in adult rats. The aim of the present study was to determine if perinatal exposure to sidestream cigarette smoke influences the composition and function of pulmonary surfactant system in developing rat pups. 2 Pregnant Sprague Dawley rats were exposed to sidestream cigarette smoke in a whole body exposure chamber for a total of 6 h each day and the pups born to these dams were further exposed to sidestream smoke for 2 h/day during postnatal period. Exposure of animals to smoke was ascertained by measuring their plasma cotinine. Surfactant analysis was performed on bronchoalveolar lavage fluids (BALF) collected from pups on postnatal day 1, 3, 7, 14, 21 and 35. The phospholipid (PL) content, percentage disaturated phosphatidylcholine (DSPC) and surfactant proteins (SP-A and SP-B) in BALF surfactant preparations from sham and smoke-exposed pups were compared. 3 Significant differences between the two groups were observed at two exposure points: a reduced level of SPA on day 1, and, a higher level of SP-A and PLs on day 21, in smoke-exposed pups. Surface activity analysis of the surfactant films by pulsating bubble surfactometer did not show any significant differences between the sham and smoke-exposed groups at any exposure point. 4 The results indicate that perinatal exposure to sidestream smoke is capable of producing biochemical changes in the composition of pulmonary surfactant at different stages of development but these changes do not influence surface tension reducing properties of the surfactant.

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Quercetin attenuates di-(2-ethylhexyl) phthalate-induced testicular toxicity in adult rats

Human & Experimental Toxicology ◽

10.1177/0960327115580602 ◽

2015 ◽

Vol 35 (3) ◽

pp. 232-243 ◽

Cited By ~ 19

Author(s):

MF Abd-Ellah ◽

HAA Aly ◽

HAM Mokhlis ◽

AH Abdel-Aziz

Keyword(s):

Oxidative Stress ◽

Corn Oil ◽

Sperm Count ◽

Serum Testosterone ◽

Prostatic Acid Phosphatase ◽

Serum Testosterone Level ◽

Testicular Toxicity ◽

Group Vi ◽

Adult Rats ◽

Ethylhexyl Phthalate

The aim of the present study was to investigate the potential oxidative damage of di-(2-ethylhexyl) phthalate (DEHP) in the rat testis and to further elucidate the potential modulatory effect of quercetin. DEHP was diluted in corn oil and given to rats by oral gavage at doses 0, 300, 600, and 900 mg/kg/day (groups I, III, IV, or V, respectively) for 15 consecutive days. Group VI was pretreated with quercetin (90 mg/kg), 24 h before starting the experiment and then treated with DEHP (900 mg/kg/day) for 15 consecutive days. Group II was treated with quercetin (90 mg/kg/day). The relative testes weight and sperm motility were significantly decreased by treatment with 900 mg/kg of DEHP. Both sperm count and daily sperm production were significantly decreased by DEHP treatment at doses of 600 and 900 mg/kg. Serum testosterone level and prostatic acid phosphatase (ACP) activity and testicular lactate dehydrogenase-X (LDH-X) activity were significantly decreased in animals treated with 900 mg/kg. Serum total ACP activity was significantly increased in animals treated with 600 and 900 mg/kg of DEHP. DEHP treatment induced oxidative stress and histopathological abnormality. These abnormalities were effectively normalized by pretreatment with quercetin except for LDH-X near normalcy. In conclusion, the findings of this study demonstrate that DEHP impairs testicular function at least, in part, by inducing oxidative stress and quercetin has a potent protective effect against DEHP-induced testicular toxicity in rats.

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