Inheritance and development of EST-SSR marker associated with turnip mosaic virus resistance in Chinese cabbage

2011 ◽  
Vol 91 (4) ◽  
pp. 707-715 ◽  
Author(s):  
Qiaoyun Li ◽  
Haishen Tong ◽  
Zhigang Zhang ◽  
Zhizhong Zhao ◽  
Xiyun Song
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Chinese Cabbage ◽  
Virus Resistance ◽  
Ssr Marker ◽  
Recessive Gene ◽  
Turnip Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
The Cross ◽  
Tumv Resistance

Li, Q., Tong, H., Zhang, Z., Zhao, Z. and Song, X. 2011. Inheritance and development of EST-SSR marker associated with turnip mosaic virus resistance in Chinese cabbage. Can. J. Plant Sci. 91: 707–715. Turnip mosaic virus (TuMV) is one of the major pathogens infecting Brassica crops (including Chinese cabbage), and often causes serious reductions in yield and quality. Breeding for resistant cultivars is complicated by the existence of numerous TuMV strains and isolates. The objective of this research was to determine the mode of inheritance and to develop molecular markers associated with TuMV resistance in Chinese cabbage. F1 and F2 populations were developed from the cross between 71-36-2 (susceptible) and 73 (resistant) lines and mechanically inoculated with TuMV-C4. Inheritance analysis by visual scoring and enzyme-linked immunosorbent assay (ELISA) indicated that resistance to TuMV-C4 in this cross was controlled by one recessive gene. A total of 132 EST-SSR primers were designed from EST sequences available in public databases. Seven primers detected polymorphism between parental genotypes. Marker HCC259 was associated with the TuMV resistance Ph-retr02. The distance between the marker and the TuMV resistance gene retr02 was 3.8 cM. This is the first co-dominant marker linked to the TuMV resistance gene with a distance less than 5.0 cM in Chinese cabbage. This marker was suitable for TuMV-C4 resistance screening in progenies from the cross between lines 73 (resistant) and susceptible line, 71-36-2. The usefulness of this marker was validated in 21 additional resistant and susceptible lines. This marker has the potential to simplify and accelerate breeding Chinese cabbage cultivars resistant to TuMV-C4.

Genetic mapping of the novel Turnip mosaic virus resistance gene TuRB03 in Brassica napus

2003 ◽  
Vol 107 (7) ◽  
pp. 1169-1173 ◽  
Author(s):  
S. L. Hughes ◽  
P. J. Hunter ◽  
A. G. Sharpe ◽  
M. J. Kearsey ◽  
D. J. Lydiate ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Turnip Mosaic Virus ◽  
The Novel ◽  
Virus Resistance Gene

Identification and mapping of a novel Turnip mosaic virus resistance geneTuRBCS01in Chinese cabbage (Brassica rapaL.)

2014 ◽  
Vol 134 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Qiaoyun Li ◽  
Xiaoliang Zhang ◽  
Qiang Zeng ◽  
Zhigang Zhang ◽  
Shuantao Liu ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Chinese Cabbage ◽  
Virus Resistance ◽  
Turnip Mosaic Virus

Genetic control of immunity to Turnip mosaic virus (TuMV) pathotype 1 in Brassica rapa (Chinese cabbage)

Genome ◽  
2014 ◽  
Vol 57 (8) ◽  
pp. 419-425 ◽  
Author(s):  
Derek J. Lydiate ◽  
Rachel L. Rusholme Pilcher ◽  
Erin E. Higgins ◽  
John A. Walsh
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Turnip Mosaic Virus ◽  
Rflp Markers ◽  
Resistance Locus ◽  
Chromosome 6 ◽  
A Genome ◽  
Virus Interactions

Turnip mosaic virus (TuMV) is the major virus infecting crops of the genus Brassica worldwide. A dominant resistance gene, TuRB01b, that confers immunity to the virus isolate UK 1 (a representative pathotype 1 isolate of TuMV) on Brassica rapa was identified in the Chinese cabbage cultivar Tropical Delight. The TuRB01b locus was mapped to a 2.9-cM interval on B. rapa chromosome 6 (A6) that was flanked by RFLP markers pN101e1 and pW137e1. This mapping used a first backcross (B1) population segregating for the resistance gene at TuRB01b and sets of RFLP markers employed in previous mapping experiments in Brassica. Virus–plant interaction phenotypes were assayed in inbred progeny derived from B1 individuals to allow different virus isolates to be tested. Comparative mapping confirmed that A6 of B. rapa was equivalent to chromosome 6 of Brassica napus (A6) and that the map position of TuRB01b in B. rapa could be identical to that of TuRB01 in B. napus. Detailed evaluation of plant–virus interactions showed that TuRB01 and TuRB01b had indistinguishable specificities to a range of TuMV isolates. The possibility that TuRB01 and TuRB01b represent similar or identical alleles at the same A genome resistance locus suggests that B. napus acquired TuRB01 from the B. rapa gene pool.

scholarly journals Resistance Against Melon Chlorotic Mosaic Virus and Tomato Leaf Curl New Delhi Virus in Melon

Plant Disease ◽  
2019 ◽  
Vol 103 (11) ◽  
pp. 2913-2919 ◽  
Author(s):  
Gustavo Romay ◽  
Michel Pitrat ◽  
Herve Lecoq ◽  
Catherine Wipf-Scheibel ◽  
Pauline Millot ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Resistance ◽  
Dominant Gene ◽  
Virus Detection ◽  
Recessive Gene ◽  
Tomato Leaf ◽  
Enzyme Linked Immunosorbent Assay ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Thirty-one melon accessions were screened for resistance to the begomoviruses Melon chlorotic mosaic virus (MeCMV) and Tomato leaf curl New Delhi virus (ToLCNDV). Five accessions presented nearly complete resistance to both viruses. Accession IC-274014, showing the highest level of resistance to both viruses, was crossed with the susceptible cultivar Védrantais. The F1, F2, F3/F4, and both backcross progenies were mechanically inoculated with MeCMV. Plants without symptoms or virus detection by enzyme-linked immunosorbent assay and/or PCR were considered as resistant. The segregations were compatible with two recessive and one dominant independent genes simultaneously required for resistance. Inheritance of resistance to ToLCNDV in the F2 was best explained by one recessive gene and two independent dominant genes simultaneously required. Some F3 and F4 families selected for resistance to MeCMV also were resistant to ToLCNDV, suggesting that common or tightly linked genes were involved in resistance to both viruses. We propose the names begomovirus resistance-1 and Begomovirus resistance-2 for these genes (symbols bgm-1 and Bgm-2). Resistance to MeCMV in IC-274014 was controlled by bgm-1, Bgm-2, and the recessive gene melon chlorotic mosaic virus resistance (mecmv); resistance to ToLCNDV was controlled by bgm-1, Bgm-2, and the dominant gene Tomato leaf curl New Delhi virus resistance (Tolcndv).

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