Identification and mapping of a novel Turnip mosaic virus resistance geneTuRBCS01in Chinese cabbage (Brassica rapaL.)

2014 ◽  
Vol 134 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Qiaoyun Li ◽  
Xiaoliang Zhang ◽  
Qiang Zeng ◽  
Zhigang Zhang ◽  
Shuantao Liu ◽  
...  

3 Biotech ◽  
2020 ◽  
Vol 10 (8) ◽  
Author(s):  
Rujia Zhang ◽  
Chang Liu ◽  
Xiaoming Song ◽  
Feifei Sun ◽  
Dong Xiao ◽  
...  




2011 ◽  
Vol 91 (4) ◽  
pp. 707-715 ◽  
Author(s):  
Qiaoyun Li ◽  
Haishen Tong ◽  
Zhigang Zhang ◽  
Zhizhong Zhao ◽  
Xiyun Song

Li, Q., Tong, H., Zhang, Z., Zhao, Z. and Song, X. 2011. Inheritance and development of EST-SSR marker associated with turnip mosaic virus resistance in Chinese cabbage. Can. J. Plant Sci. 91: 707–715. Turnip mosaic virus (TuMV) is one of the major pathogens infecting Brassica crops (including Chinese cabbage), and often causes serious reductions in yield and quality. Breeding for resistant cultivars is complicated by the existence of numerous TuMV strains and isolates. The objective of this research was to determine the mode of inheritance and to develop molecular markers associated with TuMV resistance in Chinese cabbage. F1 and F2 populations were developed from the cross between 71-36-2 (susceptible) and 73 (resistant) lines and mechanically inoculated with TuMV-C4. Inheritance analysis by visual scoring and enzyme-linked immunosorbent assay (ELISA) indicated that resistance to TuMV-C4 in this cross was controlled by one recessive gene. A total of 132 EST-SSR primers were designed from EST sequences available in public databases. Seven primers detected polymorphism between parental genotypes. Marker HCC259 was associated with the TuMV resistance Ph-retr02. The distance between the marker and the TuMV resistance gene retr02 was 3.8 cM. This is the first co-dominant marker linked to the TuMV resistance gene with a distance less than 5.0 cM in Chinese cabbage. This marker was suitable for TuMV-C4 resistance screening in progenies from the cross between lines 73 (resistant) and susceptible line, 71-36-2. The usefulness of this marker was validated in 21 additional resistant and susceptible lines. This marker has the potential to simplify and accelerate breeding Chinese cabbage cultivars resistant to TuMV-C4.



1994 ◽  
Vol 89 (5) ◽  
pp. 583-589 ◽  
Author(s):  
M. A. Robbins ◽  
H. Witsenboer ◽  
R. W. Michelmore ◽  
J. -F. Laliberte ◽  
M. G. Fortin


2003 ◽  
Vol 107 (7) ◽  
pp. 1169-1173 ◽  
Author(s):  
S. L. Hughes ◽  
P. J. Hunter ◽  
A. G. Sharpe ◽  
M. J. Kearsey ◽  
D. J. Lydiate ◽  
...  


2015 ◽  
Vol 31 (4) ◽  
pp. 363-370 ◽  
Author(s):  
Bong Nam Chung ◽  
Kyung San Choi ◽  
Jeong Joon Ahn ◽  
Jae Ho Joa ◽  
Ki Seck Do ◽  
...  


2019 ◽  
Vol 109 (5) ◽  
pp. 904-912 ◽  
Author(s):  
Junsu Gong ◽  
Hye-Kyoung Ju ◽  
Ik-Hyun Kim ◽  
Eun-Young Seo ◽  
In-Sook Cho ◽  
...  

Infectious clones were generated from 17 new Korean radish isolates of Turnip mosaic virus (TuMV). Phylogenetic analysis indicated that all new isolates, and three previously characterized Korean radish isolates, belong to the basal-BR group (indicating that the pathotype can infect both Brassica and Raphanus spp.). Pairwise analysis revealed genomic nucleotide and polyprotein amino acid identities of >87.9 and >95.7%, respectively. Five clones (HJY1, HJY2, KIH2, BE, and prior isolate R007) had lower sequence identities than other isolates and produced mild symptoms in Nicotiana benthamiana. These isolates formed three distinct sequence classes (HJY1/HJY2/R007, KIH2, and BE), and several differential amino acid residues (in P1, P3, 6K2, and VPg) were present only in mild isolates HJY1, HJY2, and R007. The remaining isolates all induced systemic necrosis in N. benthamiana. Four mild isolates formed a phylogenetic subclade separate from another subclade including all of the necrosis-inducing isolates plus mild isolate KIH2. Symptom severity in radish and Chinese cabbage genotypes was not correlated with pathogenicity in N. benthamiana; indeed, Chinese cabbage cultivar Norang was not infected by any isolate, whereas Chinese cabbage cultivar Chusarang was uniformly susceptible. Four isolates were unable to infect radish cultivar Iljin, but no specific amino acid residues were correlated with avirulence. These results may lead to the identification of new resistance genes against TuMV.





2018 ◽  
Vol 36 (2) ◽  
pp. 210-224 ◽  
Author(s):  
Changwei Zhang ◽  
Shanwu Lyu ◽  
Liwei Gao ◽  
Xiaoming Song ◽  
Yanxiao Li ◽  
...  


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