A COMPARATIVE STUDY OF METHODS OF INOCULATING ALFALFA WITH LEPTOSPHAERULINA BRIOSIANA

1964 ◽  
Vol 44 (3) ◽  
pp. 249-252 ◽  
Author(s):  
J. Waddington ◽  
A. K. Storgaard ◽  
W. C. McDonald
Keyword(s):  
Comparative Study ◽  
Filter Paper ◽  
New Method ◽  
Inoculation Methods ◽  
Vegetable Juice ◽  
Inoculation Technique ◽  
Agar Method ◽  
Frozen State

An inoculation technique, in which cultures of Leptosphaerulina briosiana (Poll.) Graham & Luttrell grown on filter paper soaked in 20% mixed vegetable juice, V-8 brand, are suspended over alfalfa plants and allowed to discharge spores onto them, was developed and compared with previously described inoculation methods. The new method was found to be superior and the inoculum, in contrast to that of the similar V-8 agar method, was more easily prepared and applied, could be watered to prevent drying our and could be stored in a frozen state between inoculations. Less than 5% of the seedlings and mature plants of four species of Medicago inoculated by this method appeared to be resistant.

Cell Block Optimization: A Comparative Study of Quality Variables in 4 Different Cell Block Methods

2021 ◽  
pp. 1-7
Author(s):  
Marsali Newman ◽  
Matthew Walsh ◽  
Rosemary Jeffrey ◽  
Richard Hiscock
Keyword(s):  
Comparative Study ◽  
Processing Time ◽  
Morphological Features ◽  
Cell Block ◽  
Total Processing Time ◽  
Block Methods ◽  
Molecular Studies ◽  
Agar Method ◽  
Md Anderson ◽  
Ancillary Studies

<b><i>Objective:</i></b> The cell block (CB) is an important adjunct to cytological preparations in diagnostic cytopathology. Optimizing cellular material in the CB is essential to the success of ancillary studies such as immunohistochemistry (IHC) and molecular studies (MS). Our aim was to identify which CB method was most suitable in a variety of specimen types and levels of cellularity. <b><i>Study Design:</i></b> We assessed 4 different CB methods, thrombin clot method (TCM), MD Anderson method (MDAM), gelatin foam method (GFM), and agar method (AM), with descriptive observations and ranking of the methods based on quantity of cells and morphological features. <b><i>Results:</i></b> TCM performed best in ranking for both quantity of cells and morphological features, followed by MDAM, GFM, and AM. Lack of adjuvant in the MDAM resulted in some unique morphological advantages which, however, also resulted in inconsistent performance. In low cellularity cases insufficient cells were frequently identified on slides from MDAM and AM CBs. Technique touch time was similar for all methods, with total processing time being shortest for TCM followed by MDAM, GFM, and AM. <b><i>Conclusions:</i></b> TCM was the most robust CB technique, retaining high scores for ranking of quantity and morphology in a variety of specimen cellularities and specimen types.

Cotton Mather Anguishes Over the Consequences of His Son's Inoculation Against Smallpox

PEDIATRICS ◽  
1974 ◽  
Vol 53 (5) ◽  
pp. 756-756
Author(s):  
T. E. C.
Keyword(s):  
Royal Society ◽  
New Method ◽  
Poor People ◽  
Inoculation Technique ◽  
Cotton Mather ◽  
The People ◽  
Small Pox ◽  
Contemporary Science ◽  
Historical Writings ◽  
The Town

Cotton Mather (1663-1728), usually remembered for his theological and historical writings, was also much concerned with medicine. He was interested in many aspects of contemporary science and became one of the few colonial members of the Royal Society of London. In 1721, when a smallpox epidemic hit Boston, Mather urged Boston physicians, particularly Zabdiel Boylston, to employ the inoculation technique used by the Turks as a means of preventing fatal cases of the disease. In his Diary, Mather records the anguish he suffered for having taken this stand. [May] 26 [1721]. The grievous Calamity of the Small-Pox has now entered the Town. The Practice of conveying and suffering the Small-pox by Inoculation, has never been used in America, nor indeed in our Nation, But how many Lives might be saved by it, if it were practised? . . . [June] 13. What shall I do? what shall I do, with regard unto Sammy? He comes home, when the Small-pox begins to spread in the Neighbourhood; and he is lothe to return unto Cambridge. I must earnestly look up to Heaven for Direction. . . . [July] 16. At this Time, I enjoy an unspeakable Consolation. I have instructed our Physicians in the new Method used by the Africans and Asiaticks, to prevent and abate the Dangers of the Small-Pox, and infallibly to save the Lives of those that have it wisely managed upon them. The Destroyer, being enraged at the Proposal of any Thing, that may rescue the Lives of our poor People from him, has taken a strange Possession of the People on this Occasion.

Comparative study of Nd:GdVO4 and Nd:YVO4, and test of a composite Nd:YVO4/YVO4 rod using a new method of bonding

2006 ◽  
Author(s):  
Julien Didierjean ◽  
François Balembois ◽  
Frédéric Druon ◽  
Patrick Georges ◽  
Johan Petit ◽  
...  
Keyword(s):  
Comparative Study ◽  
New Method

An improved method for determining the nitrogen solubility of caseinates

1990 ◽  
Vol 57 (2) ◽  
pp. 207-212
Author(s):  
Wolfgang Kneifel ◽  
Anne Beurel
Keyword(s):  
Filter Paper ◽  
Nitrogen Solubility ◽  
New Method ◽  
Nitrogen Solubility Index ◽  
Improved Method

SummaryThe nitrogen solubility index (NSI) method for the examination of caseinates was evaluated. It was found that widely scattering NSI values are mainly due to ineffective centrifugation and filtration of the caseinate dispersion. In order to avoid this, an improved combined centrifugation–filtration procedure was developed as an alternative to the original NSI method, using an Amicon micropartition system MPS-1 equipped with discs of filter paper. The micropartition devices can be centrifuged in a Gerber centrifuge of the type used for butyromctric fat determination. Several types of caseinates were evaluated with the new method and yielded a markedly smaller range of single values than were obtained with the NSI method.

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