COMPARISON OF MEDIA FOR THE ISOLATION OF FUSARIUM OXYSPORUM F. SP. LYCOPERSICI FROM SAWDUST USED FOR GROWING TOMATOES

1974 ◽  
Vol 54 (2) ◽  
pp. 373-374
Author(s):  
M. G. ANDERSON ◽  
R. G. ATKINSON
Keyword(s):  
Lycopersicon Esculentum ◽  
Fusarium Oxysporum ◽  
Rose Bengal ◽  
Antimicrobial Agents ◽  
Potato Dextrose Agar ◽  
Selective Isolation ◽  
Streptomycin Sulphate ◽  
Lycopersicon Esculentum Mill ◽  
Agar Media ◽  
Greenhouse Tomatoes

Various agar media amended with antimicrobial agents were compared for the selective isolation of Fusarium oxysporum f.sp. lycopersici from sawdust used for growing greenhouse tomatoes (Lycopersicon esculentum Mill.). Potato dextrose agar amended with pentachloronitrobenzene (PCNB) (500 mg/l) and streptomycin sulphate (300 mg/l proved to be the most satisfactory medium, tested. These antimicrobial agents, unlike rose bengal, did not reduce the number of Fusarium colonies isolated.

SULPHUR DEFICIENCY AND TOXICITY SYMPTOMS IN GREENHOUSE TOMATOES AND CUCUMBERS

1976 ◽  
Vol 56 (1) ◽  
pp. 133-137 ◽  
Author(s):  
G. M. WARD
Keyword(s):  
Lycopersicon Esculentum ◽  
Plant Tissue ◽  
Fruit Production ◽  
Sand Culture ◽  
Plant Tissues ◽  
Morphological Effect ◽  
Sulphur Deficiency ◽  
Lycopersicon Esculentum Mill ◽  
Severe Deficiency ◽  
Greenhouse Tomatoes

Symptoms of sulphur deficiency and toxicity on greenhouse tomatoes (Lycopersicon esculentum Mill.) and cucumbers (Cucumis sativis L.) were produced experimentally in sand culture and are described in detail. The principal morphological effect was a general depression of growth and fruit production. A S content of less than 0.25% in any plant tissue was associated with severe deficiency. The distribution of S in various plant tissues is shown.

scholarly journals First Report of Mimosa Wilt Disease of Silk Tree (Albizia julibrissin) in California Caused by Fusarium oxysporum f. sp. Perniciosum

Plant Disease ◽  
1999 ◽  
Vol 83 (5) ◽  
pp. 487-487 ◽  
Author(s):  
R. B. Nesbitt ◽  
T. E. Tidwell ◽  
R. J. Stipes ◽  
G. J. Griffin
Keyword(s):  
Fusarium Oxysporum ◽  
Lateral Roots ◽  
Residential Area ◽  
Wilt Disease ◽  
Potato Dextrose Agar ◽  
Water Suspension ◽  
Albizia Julibrissin ◽  
First Report ◽  
Agar Media ◽  
Fusarium Sp

A Fusarium sp. was isolated from a 12-year-old Silk Tree (Albizia julibrissin) in a residential area of Redlands, CA. The scaffold branches and trunk exhibited gummosis, the sap oozing from fissures or intact bark. Internally the wood exhibited brown to black broad streaks of discoloration from the scaffold branches down into lateral roots below the root crown, similar to symptoms observed in Virginia (2). Wilted and dried foliage remained on the scaffold branches. Two-week-old cultures of the isolate grown on Komada (1) and acidified potato dextrose agar media developed short conidiophores, macroconidia, and colony morphology typical of Fusarium oxysporum. To complete Koch's postulates, 1-month-old seedlings were root-dip inoculated with a water suspension of macro- and microconidia (106 per ml). Two weeks after inoculation, typical Fusarium wilt symptoms developed in all inoculated seedlings. The fungus was reisolated from symptomatic seedlings. This is the first report of mimosa wilt disease in California. The disease has the potential to adversely impact California's nursery and landscape industry. References: (1) H. Komada. Rev. Plant Prot. Res. 8:114, 1975. (2) R. J. Stipes and P. M. Phipps. Phytopathology 65:188, 1975.

SEMI-SELECTIVE MEDIUM FOR ISOLATION OF COLLETOTRICHUM GLOEOSPORIOIDES FROM SOIL

2000 ◽  
Vol 36 (3) ◽  
pp. 313-321 ◽  
Author(s):  
E. J. EKEFAN ◽  
S. A. SIMONS ◽  
A. O. NWANKITI ◽  
J. C. PETERS
Keyword(s):  
Antimicrobial Agents ◽  
Colletotrichum Gloeosporioides ◽  
Basal Medium ◽  
Selective Inhibition ◽  
Selective Medium ◽  
Potato Dextrose Agar ◽  
Streptomycin Sulphate ◽  
Fast Growing ◽  
Suitable Combination ◽  
Micro Organisms

Isolation of Colletotrichum gloeosporioides (causal agent of yam anthracnose) from soil is difficult because of the presence of fast growing micro-organisms like Rhizoctonia spp., Rhizopus spp., Penicillium spp. and bacteria. Therefore, a semi-selective medium consisting of antimicrobial agents such as pencycuron 50 mg L−1, tolclofos-methyl 10 mg L−1, streptomycin sulphate 100 mg L−1, chloramphenicol 100 mg L−1 and chlortetracycline 100 mg L−1 was developed using potato dextrose agar as the basal medium. The procedure involved screening of candidate fungicides, developing a suitable combination of antimicrobial agents, assessing the proposed semi-selective medium and adjustment of pH of the medium to 5. The effectiveness of the medium is due to selective inhibition of micro-organisms by the antimicrobial agents and the development of distinct salmon-pink colonies of C. gloeosporioides.

PLANT SPACING EFFECTS ON LIGHT INTERCEPTION BY GREENHOUSE TOMATOES

1988 ◽  
Vol 68 (4) ◽  
pp. 1197-1208 ◽  
Author(s):  
ATHANASIOS P. PAPADOPOULOS ◽  
DOUGLAS P. ORMROD
Keyword(s):  
Lycopersicon Esculentum ◽  
Sampling Period ◽  
Light Interception ◽  
Test Plant ◽  
Plant Spacing ◽  
Lycopersicon Esculentum Mill ◽  
Plant Canopies ◽  
Spacing Effects ◽  
Direct Light ◽  
Greenhouse Tomatoes

The effect of six equidistant spacings (23, 30, 38, 45, 53, 60 cm) on interception of photosynthetically active radiation (PAR) by four-row plantings of greenhouse tomato (Lycopersicon esculentum Mill. ’Jumbo’ and ’CR-6’) was studied. A silicon cell sensor was used to measure PAR in and around the test plant canopies. These observations were used to construct canopy profiles of PAR absorption, as affected by plant spacing and cultivar. Measurements under both clear and overcast skies revealed no major differences in the pattern of PAR interception within a canopy due to the presence or absence of direct light over the sampling period. The proportion of available PAR intercepted increased with closer plant spacing, but PAR penetration increased and distribution improved with increasing plant spacing. An increasing degree of self-shading of the central and north parts of the canopies was evident with closer planting. Plant spacing also had a large effect on the quality of canopy transmitted light. The ratios of blue:far-red, red:far-red and blue:red light transmitted through the four-row canopies of both cultivars decreased with decreases in plant spacing and in general were lower under the canopies of CR-6, than under the canopies of Jumbo.Key words: Tomato, Lycopersicon esculentum Mill., plant spacing, light interception

scholarly journals Fungi associated with egg masses and females of plant parasitic nematode Meloidogyne incognita (Nematoda: Heteroderidae)

2018 ◽  
Vol 44 (3) ◽  
pp. 373-378 ◽  
Author(s):  
Mehmet Karakas
Keyword(s):  
Lycopersicon Esculentum ◽  
Fusarium Oxysporum ◽  
Meloidogyne Incognita ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Central Anatolia ◽  
Egg Masses ◽  
Predominant Species ◽  
Lycopersicon Esculentum Mill ◽  
Plant Parasitic

Egg masses and females of Meloidogyne incognita from a tomato (Lycopersicon esculentum Mill. cv. Falcon) field from Central Anatolia of Turkey, were collected and examined for the presence of fungi. Of the 138 egg masses of M. incognita 35.5% were colonized by one or more of 9 species of genera Arthrobotrys, Cylindrocarpon, Fusarium, Paecilomyces, Plectosphaerella, Rhizoctonia and Verticillium while 31.8% of the 138 females were colonized by 6 species, from the same genera except Paecilomyces and Plectosphaerella. Fusarium oxysporum was by far the predominant species in both the egg masses and females of M. incognita.

scholarly journals Selectivity of Fusarium Culture Filtrates in Agar Media

1969 ◽  
Vol 53 (2) ◽  
pp. 140-146
Author(s):  
R. Alconero ◽  
Alma G. Santiago
Keyword(s):  
Fusarium Oxysporum ◽  
Spore Germination ◽  
Linear Growth ◽  
Colony Development ◽  
Selective Isolation ◽  
Potato Dextrose Broth ◽  
Culture Filtrates ◽  
Agar Media

Filtrates of Fusarium oxysporum f. sp. vanillae (Tucker) Gordon, Schlecht. and two isolates of F. solani (Mart.) Appel & Wr., grown in nutrient and potato dextrose broth, effected spore germination, linear growth in agar, and colony development in soil plates of these and other fungi. There was not enough sign of selectivity in these filtrates to warrant their use for selective isolation of fungi from soil.

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