Preparation and Characterization of Water-Insoluble Gardenia Blue Pigment

Based on molecular simulations, the synthetic route of water-insoluble gardenia blue pigment was prepared by the reaction of genipin and L-Phenylalanine methyl ester hydrochloride. A highly purified pigment was obtained after extraction by chloroform and purification by silica gel column chromatography, and the value of color is up to 288. A study on the structural characteristics of the pigment was implemented with a scanning electron microscope, ultraviolet-visible spectrophotometer, Fourier transform infrared spectrometer, X-ray photoelectron spectrometer, and quatropde-time of flight mass spectrometer. The results showed that the surface of the pigment was largely smooth and spherical; The λmax was 607 nm, and the main functional groups include O-C=O, C=O, C-N, C=C, OH, and benzene ring; We detrained six different molecular weight and chemical structures of pigments and speculated the particular structures and formation mechanisms of three kinds of pigment, whose molecular weights are 690.1156, 720.1226, and 708.1246 Da, respectively. The pigment was only able to be dissolved in ethanol, methanol, acetone, ethyl acetate, and other strong polar organic solvents, but was not able to be dissolved in water, ethyl ether, petroleum ether, and other weak polar organic solvents. In terms of light and thermal stabilities, water-insoluble gardenia blue pigment is significantly better than water-soluble gardenia blue pigment (p < 0.05). When it is under direct light for 7 days or incubated at 80–120 °C for 24 h, the pigment residual rates were 74.90, 95.26, 88.27, and 87.72%, respectively.

Density and habitat characterization of Aedes sp. breeding places in dengue Endemic Area in Bandar Lampung

Aedes sp. are vectors of the dengue virus that causes Dengue Haemorrhagic Fever (DHF). The purposed of this study to analyze habitat characteristics of Aedes sp. based on the entomological index and measure the density and species of larvae Aedes sp. The research was analytic observational with a cross-sectional study was conducted 3 subdistricts of endemic in Bandar Lampung. The results showed that from 3 locations, namely Sukarame, Kemiling and Tanjung Seneng, the House Index (HI) values were 41, 38, 31, respectively. The Container Index (CI) values were 14, 12, 10 respectively and the Breteau index (BI) values were 105, 77, 60 respectively. The result of density figure (DF) were 5-6 which means the density of Aedes sp. were moderate to high. The result of identification of larvae were 3 species of mosquito were Aedes aegypti, Aedes albopictus and Culex quinquefasciatus. The dominant spesies of larvae were found are Ae. aegypti. The characteristics of larvae habitat as a risk factor for increasing the density of mosquito in Bandar Lampung are buckets or container made of plastic or cement and dispenser, light colour, direct light exposure, 1-20 liters water volume, no cover container, no drainage, and no fish rearing.

Additive Manufacturing for Neurosurgery: Digital Light Processing of Individualized Patient-Specific Cerebral Aneurysms

This study aims at demonstrating the feasibility of reproducing individualized patient-specific three-dimensional models of cerebral aneurysms by using the direct light processing (DLP) 3D printing technique in a low-time and inexpensive way. Such models were used to help neurosurgeons understand the anatomy of the aneurysms together with the surrounding vessels and their relationships, providing, therefore, a tangible supporting tool with which to train and plan surgical operations. The starting 3D models were obtained by processing the computed tomography angiographies and the digital subtraction angiographies of three patients. Then, a 3D DLP printer was used to print the models, and, if acceptable, on the basis of the neurosurgeon’s opinion, they were used for the planning of the neurosurgery operation and patient information. All the models were printed within three hours, providing a comprehensive representation of the cerebral aneurysms and the surrounding structures and improving the understanding of their anatomy and simplifying the planning of the surgical operation.

Data-Driven Object Pose Estimation in a Practical Bin-Picking Application

This paper addresses the problem of pose estimation from 2D images for textureless industrial metallic parts for a semistructured bin-picking task. The appearance of metallic reflective parts is highly dependent on the camera viewing direction, as well as the distribution of light on the object, making conventional vision-based methods unsuitable for the task. We propose a solution using direct light at a fixed position to the camera, mounted directly on the robot’s gripper, that allows us to take advantage of the reflective properties of the manipulated object. We propose a data-driven approach based on convolutional neural networks (CNN), without the need for a hard-coded geometry of the manipulated object. The solution was modified for an industrial application and extensively tested in a real factory. Our solution uses a cheap 2D camera and allows for a semi-automatic data-gathering process on-site.

Embedding of Ultrathin Chips in Highly Flexible, Photosensitive Solder Mask Resist

This work presents an embedding process for ultrathin silicon chips in mechanically flexible solder mask resist and their electrical contacting by inkjet printing. Photosensitive solder mask resist is applied by conformal spray coating onto epoxy bonded ultrathin chips with a daisy chain layout. The contact pads are opened by photolithography using UV direct light exposure. Circular and rectangular openings of 90 µm and 130 µm diameter, respectively, edge length are realized. Commercial inks containing nanoparticular silver and gold are inkjet printed to form conductive tracks between daisy chain structures. Different numbers of ink layers are applied. The track resistances are characterized by needle probing. Silver ink shows low resistances only for multiple layers and 90 µm openings, while gold ink exhibits low resistances in the single-digit Ω-range for minimum two printed layers.

Dissecting and modeling photic and melanopsin effects to predict sleep disturbances induced by irregular light exposure in mice

Artificial lighting, day-length changes, shift work, and transmeridian travel all lead to sleep–wake disturbances. The nychthemeral sleep–wake cycle (SWc) is known to be controlled by output from the central circadian clock in the suprachiasmatic nuclei (SCN), which is entrained to the light–dark cycle. Additionally, via intrinsically photosensitive retinal ganglion cells containing the photopigment melanopsin (Opn4), short-term light–dark alternations exert direct and acute influences on sleep and waking. However, the extent to which longer exposures typically experienced across the 24-h day exert such an effect has never been clarified or quantified, as disentangling sustained direct light effects (SDLE) from circadian effects is difficult. Recording sleep in mice lacking a circadian pacemaker, either through transgenesis (Syt10cre/creBmal1fl/-) or SCN lesioning and/or melanopsin-based phototransduction (Opn4−/−), we uncovered, contrary to prevailing assumptions, that the contribution of SDLE is as important as circadian-driven input in determining SWc amplitude. Specifically, SDLE were primarily mediated (>80%) through melanopsin, of which half were then relayed through the SCN, revealing an ancillary purpose for this structure, independent of its clock function in organizing SWc. Based on these findings, we designed a model to estimate the effect of atypical light–dark cycles on SWc. This model predicted SWc amplitude in mice exposed to simulated transequatorial or transmeridian paradigms. Taken together, we demonstrate this SDLE is a crucial mechanism influencing behavior on par with the circadian system. In a broader context, these findings mandate considering SDLE, in addition to circadian drive, for coping with health consequences of atypical light exposure in our society.

Afraid of the dark: Light acutely suppresses activity in the human amygdala

Light improves mood. The amygdala plays a critical role in regulating emotion, including fear-related responses. In rodents the amygdala receives direct light input from the retina, and light may play a role in fear-related learning. A direct effect of light on the amygdala represents a plausible mechanism of action for light’s mood-elevating effects in humans. However, the effect of light on activity in the amygdala in humans is not well understood. We examined the effect of passive dim-to-moderate white light exposure on activation of the amygdala in healthy young adults using the BOLD fMRI response (3T Siemens scanner; n = 23). Participants were exposed to alternating 30s blocks of light (10 lux or 100 lux) and dark (<1 lux), with each light intensity being presented separately. Light, compared with dark, suppressed activity in the amygdala. Moderate light exposure resulted in greater suppression of amygdala activity than dim light. Furthermore, functional connectivity between the amygdala and ventro-medial prefrontal cortex was enhanced during light relative to dark. These effects may contribute to light’s mood-elevating effects, via a reduction in negative, fear-related affect and enhanced processing of negative emotion.

Development and Testing of a Dual-Wavelength Sensitive Photopolymer Layer for Applications in Stacking of HOE Lenses

Diffractive optical elements (DOEs) have been in development for many years and are an exciting technology with the capability to re-direct light, using diffraction rather than refraction. Holographic Optical Elements (HOEs) are a subset of diffractive optical elements for which the photonic structure is created holographically, i.e., by recording a specific interference pattern in a suitable, photosensitive optical material. Volume HOEs are of particular interest for some applications because of their very high diffraction efficiency and single diffracted order; however, high dispersion and angular wavelength selectivity still present significant challenges. This paper explores a method for producing a compound DOE useful for situations where elements designed for two separate target wavelengths can be advantageously combined to achieve a highly efficient HOE with reduced dispersion. A photopolymer material consisting of two independently sensitized laminated layers is prepared and used in sequential holographic recording at two different wavelengths. The photonic structures recorded are investigated through examination of their diffraction peaks and comparison with the structure predicted by modeling. Finally, the device is illuminated with an expanded diverging beam at both target wavelengths and with white light, and a strong diffracted beam is observed.

Real-Time PCR as an Alternative Technique for Detection of Dermatophytes in Cattle Herds

Dermatophytes are filamentous fungi with the ability to digest and grow on keratinized substrates. The ongoing improvements in fungal detection techniques give new scope for clinical implementations in laboratories and veterinary clinics, including the monitoring of the disease and carrier status. The technologically advanced methods for dermatophyte detection include molecular methods based on PCR. In this context, the aim of this study was to carry out tests on the occurrence of dermatophytes in cattle herds using qPCR methods and a comparative analysis with conventional methods. Each sample collected from ringworm cases and from asymptomatic cattle was divided into three parts and subjected to the real-time PCR technique, direct light microscopy analysis, and culture-based methods. The use of the real-time PCR technique with pan-dermatophyte primers detected the presence of dermatophytes in the sample with a 10.84% (45% vs. 34.17%) higher efficiency than direct analysis with light microscopy. Moreover, a dermatophyte culture was obtained from all samples with a positive qPCR result. In conclusion, it seems that this method can be used with success to detect dermatophytes and monitor cowsheds in ringworm cases and carriers in cattle.