LC–MS/MS method for denosumab quantitation in human serum with rapid protein digestion using immobilized trypsin

Bioanalysis ◽  
2018 ◽  
Vol 10 (18) ◽  
pp. 1501-1510 ◽  
Author(s):  
Hiroaki Shida ◽  
Takafumi Naito ◽  
Kaito Shibata ◽  
Yasuhide Yamada ◽  
Junichi Kawakami
Keyword(s):  
Human Serum ◽  
Protein Digestion ◽  
Immobilized Trypsin

Simple and rapid LC-MS/MS method for the absolute determination of cetuximab in human serum using an immobilized trypsin

2017 ◽  
Vol 146 ◽  
pp. 266-272 ◽  
Author(s):  
Kaito Shibata ◽  
Takafumi Naito ◽  
Jun Okamura ◽  
Seiji Hosokawa ◽  
Hiroyuki Mineta ◽  
...  
Keyword(s):  
Human Serum ◽  
Absolute Determination ◽  
Immobilized Trypsin ◽  
The Absolute

Quantitative LC-MS/MS method for nivolumab in human serum using IgG purification and immobilized tryptic digestion

2020 ◽  
Vol 12 (1) ◽  
pp. 54-62 ◽  
Author(s):  
Kazuki Abe ◽  
Kaito Shibata ◽  
Takafumi Naito ◽  
Masato Karayama ◽  
Etsuko Hamada ◽  
...  
Keyword(s):  
Human Serum ◽  
Tryptic Digestion ◽  
Immobilized Trypsin ◽  
Igg Purification

A simple and rapid LC-MS/MS method for quantification of nivolumab in human serum using immobilized trypsin coupled to IgG purification.

Facile synthesis of enzyme–inorganic hybrid nanoflowers and their application as an immobilized trypsin reactor for highly efficient protein digestion

RSC Advances ◽  
2014 ◽  
Vol 4 (27) ◽  
pp. 13888-13891 ◽  
Author(s):  
Zian Lin ◽  
Yun Xiao ◽  
Ling Wang ◽  
Yuqing Yin ◽  
Jiangnan Zheng ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Immobilized Enzyme ◽  
Protein Digestion ◽  
Enzyme Reactor ◽  
Facile Synthesis ◽  
Inorganic Hybrid ◽  
Immobilized Enzyme Reactor ◽  
Highly Efficient ◽  
Novel Approach ◽  
Immobilized Trypsin

Hybrid nanoflowers were synthesized by a novel approach. The nanoflowers exhibited an enhanced enzymatic activity and can be used as an immobilized enzyme reactor (IMER) for highly efficient protein digestion.

Rational synthesis of MoS2-based immobilized trypsin for rapid and effective protein digestion

Talanta ◽  
2018 ◽  
Vol 179 ◽  
pp. 393-400 ◽  
Author(s):  
Chaoshuang Xia ◽  
Heping Wang ◽  
Fenglong Jiao ◽  
Fangyuan Gao ◽  
Qiong Wu ◽  
...  
Keyword(s):  
Protein Digestion ◽  
Immobilized Trypsin
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