Quantitative LC-MS/MS method for nivolumab in human serum using IgG purification and immobilized tryptic digestion

2020 ◽  
Vol 12 (1) ◽  
pp. 54-62 ◽  
Author(s):  
Kazuki Abe ◽  
Kaito Shibata ◽  
Takafumi Naito ◽  
Masato Karayama ◽  
Etsuko Hamada ◽  
...  
Keyword(s):  
Human Serum ◽  
Tryptic Digestion ◽  
Immobilized Trypsin ◽  
Igg Purification

A simple and rapid LC-MS/MS method for quantification of nivolumab in human serum using immobilized trypsin coupled to IgG purification.

Simple and rapid LC-MS/MS method for the absolute determination of cetuximab in human serum using an immobilized trypsin

2017 ◽  
Vol 146 ◽  
pp. 266-272 ◽  
Author(s):  
Kaito Shibata ◽  
Takafumi Naito ◽  
Jun Okamura ◽  
Seiji Hosokawa ◽  
Hiroyuki Mineta ◽  
...  
Keyword(s):  
Human Serum ◽  
Absolute Determination ◽  
Immobilized Trypsin ◽  
The Absolute

A new process of IgG purification by negative chromatography: Adsorption aspects of human serum proteins onto ω-aminodecyl-agarose

2010 ◽  
Vol 878 (23) ◽  
pp. 2087-2093 ◽  
Author(s):  
Igor Tadeu Lazzarotto Bresolin ◽  
Maria Cristiane Martins de Souza ◽  
Sonia Maria Alves Bueno
Keyword(s):  
Human Serum ◽  
Serum Proteins ◽  
New Process ◽  
Igg Purification ◽  
Human Serum Proteins

Tryptic digestion of human serum for proteomic mass spectrometry automated by centrifugal microfluidics

Lab on a Chip ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 2937-2946
Author(s):  
J.-N. Klatt ◽  
M. Depke ◽  
N. Goswami ◽  
N. Paust ◽  
R. Zengerle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Serum ◽  
Centrifugal Microfluidics ◽  
Tryptic Digestion

Tryptic digestion of human serum automated by centrifugal microfluidics.

IgG purification to measure the level of an iodinated thyroglobulin peptide, the 3,5,3′,5′ tetraiodo-l-tyrosyl-l-tyrosine in human serum

1985 ◽  
Vol 41 (3) ◽  
pp. 385-387
Author(s):  
D. Bernard ◽  
J. -C. Maurizis ◽  
C. Nicolas ◽  
J. Michelot ◽  
G. Bétail ◽  
...  
Keyword(s):  
Human Serum ◽  
Igg Purification

Hollow silica bubble based immobilized trypsin for highly efficient proteome digestion and buoyant separation

RSC Advances ◽  
2016 ◽  
Vol 6 (87) ◽  
pp. 84113-84118 ◽  
Author(s):  
Fenglong Jiao ◽  
Rui Zhai ◽  
Junjie Huang ◽  
Yukui Zhang ◽  
Yangjun Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tryptic Digestion ◽  
Hollow Silica ◽  
Highly Efficient ◽  
Immobilized Trypsin ◽  
Identification And Quantification

Tryptic digestion before identification and quantification by mass spectrometry is an indispensable process for most proteomics studies.

LC–MS/MS method for denosumab quantitation in human serum with rapid protein digestion using immobilized trypsin

Bioanalysis ◽  
2018 ◽  
Vol 10 (18) ◽  
pp. 1501-1510 ◽  
Author(s):  
Hiroaki Shida ◽  
Takafumi Naito ◽  
Kaito Shibata ◽  
Yasuhide Yamada ◽  
Junichi Kawakami
Keyword(s):  
Human Serum ◽  
Protein Digestion ◽  
Immobilized Trypsin
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