Simultaneous estimation of ZY-19489 and its active metabolite ZY-20486 in human plasma using LC–MS/MS, a novel antimalarial compound

Bioanalysis ◽  
2021 ◽  
Author(s):  
Ashok M Ghoghari ◽  
Harilal V Patel ◽  
Nisarg N Nayak ◽  
Tariq H Mansuri ◽  
Soma M Pillai ◽  
...  
Keyword(s):  
Human Plasma ◽  
Clinical Sample ◽  
Human Subjects ◽  
Internal Standard ◽  
Ammonia Solution ◽  
Drug Candidate ◽  
Simultaneous Estimation ◽  
Healthy Human ◽  
Healthy Human Subjects ◽  
Ionization Mode

Aim: ZY-19489 is a new antimalarial drug candidate and selective LC–MS/MS method was established for estimation of ZY-19489 and its metabolite in human plasma. Materials & methods: LLE was employed for extraction, mass spectrometric quantification performed using positive ionization mode and DCP-IMP was used as an internal standard. The chromatographic separation was achieved using mobile phase 5 mM ammonium formate in water and 0.1% v/v ammonia solution in methanol: acetonitrile (90: 10%, v/v) and column Agilent Zorbex Extended C18, 3.5 μm, 100 × 4.6 mm with a 6-min run time. Results: The calibration curve of ZY-19489 was linear over range 1–500 ng/ml and 2–200 ng/ml for metabolite. Assay was reproducible, selective and devoid of matrix effect. Conclusion: The validated assay was implemented for clinical sample analysis derived from healthy human subjects and parasitemia-induced subjects.

scholarly journals Quantification of Paroxetine in Human Plasma by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry

2010 ◽  
Vol 93 (1) ◽  
pp. 141-149 ◽  
Author(s):  
Hiten J Shah ◽  
Mohan L Kundlik ◽  
Abhijit Kakad ◽  
Nitesh K Patel ◽  
Ankit Pandya ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Human Plasma ◽  
Solid Phase ◽  
Human Subjects ◽  
Internal Standard ◽  
Bioequivalence Study ◽  
Selected Reaction Monitoring ◽  
Healthy Human ◽  
Healthy Human Subjects ◽  
Imipramine Hydrochloride

Abstract A rapid LC coupled with electrospray ionization (ESI) MS/MS method was developed and validated for the quantification of paroxetine in heparinized human plasma. The plasma samples were prepared by the solid-phase extraction method without drying or reconstitution. Elution was done with 0.5 mL 0.2 (v/v) formic acid in methanolacetonitrile (65 + 35, v/v). The analyte and the internal standard (IS; imipramine hydrochloride) were chromatographed on a BDS Hypersil C18 column. The analyte was analyzed by LC/MS/MS with only 1.7 min run time. An ESI interface was chosen for ionization of the analyte from the sample matrix. Selected reaction monitoring mode for detection of paroxetine and the IS were achieved by using m/z 330.17/192.10 and 281.13/86.14, respectively. The LC retention times for paroxetine and imipramine were 0.94 and 1.05 min, respectively. The method was linear in the concentration range of 0.580.0 ng/mL with r ≥0.9995. Recovery of paroxetine and imipramine ranged from 90 to 95. The assay has been successfully applied to bioequivalence study samples for estimation of paroxetine in healthy human subjects.

Chronic stress decreases circulating endocannabinoid 2-arachidonoylglycerol in healthy human subjects

2015 ◽  
Author(s):  
Buqing Yi ◽  
Igor Nichiporuk ◽  
Matthias Feuerecker ◽  
Gustav Schelling ◽  
Alexander Chouker
Keyword(s):  
Chronic Stress ◽  
Human Subjects ◽  
Healthy Human ◽  
Healthy Human Subjects

scholarly journals Acacia Gum Is Well Tolerated While Increasing Satiety and Lowering Peak Blood Glucose Response in Healthy Human Subjects

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 618
Author(s):  
Riley Larson ◽  
Courtney Nelson ◽  
Renee Korczak ◽  
Holly Willis ◽  
Jennifer Erickson ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Human Subjects ◽  
Area Under The Curve ◽  
Soluble Fiber ◽  
Healthy Human ◽  
Glucose Response ◽  
Blood Glucose Response ◽  
Acacia Gum ◽  
Fiber Treatment ◽  
Healthy Human Subjects

Acacia gum (AG) is a non-viscous soluble fiber that is easily incorporated into beverages and foods. To determine its physiological effects in healthy human subjects, we fed 0, 20, and 40 g of acacia gum in orange juice along with a bagel and cream cheese after a 12 h fast and compared satiety, glycemic response, gastrointestinal tolerance, and food intake among treatments. Subjects (n = 48) reported less hunger and greater fullness at 15 min (p = 0.019 and 0.003, respectively) and 240 min (p = 0.036 and 0.05, respectively) after breakfast with the 40 g fiber treatment. They also reported being more satisfied at 15 min (p = 0.011) and less hungry with the 40 g fiber treatment at 30 min (p = 0.012). Subjects reported more bloating, flatulence, and GI rumbling on the 40 g fiber treatment compared to control, although values for GI tolerance were all low with AG treatment. No significant differences were found in area under the curve (AUC) or change from baseline for blood glucose response, although actual blood glucose with 20 g fiber at 30 min was significantly less than control. Individuals varied greatly in their postprandial glucose response to all treatments. AG improves satiety response and may lower peak glucose response at certain timepoints, and it is well tolerated in healthy human subjects. AG can be added to beverages and foods in doses that can help meet fiber recommendations.

Diaphragmatic Fatigue after Exercise in Healthy Human Subjects

1993 ◽  
Vol 148 (6_pt_1) ◽  
pp. 1571-1575 ◽  
Author(s):  
M. Jeffery Mador ◽  
Ulysses J. Magalang ◽  
Angel Rodis ◽  
Thomas J. Kufel
Keyword(s):  
Human Subjects ◽  
Healthy Human ◽  
Healthy Human Subjects ◽  
Diaphragmatic Fatigue

Capsaicin and glucose absorption and utilization in healthy human subjects

2006 ◽  
Vol 534 (1-3) ◽  
pp. 280-283 ◽  
Author(s):  
András Dömötör ◽  
János Szolcsányi ◽  
Gyula Mózsik
Keyword(s):  
Human Subjects ◽  
Glucose Absorption ◽  
Healthy Human ◽  
Healthy Human Subjects
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