This study aimed to isolate and characterize the acid soluble collagen (ASC) and pepsin
soluble collagen (PSC) from the skin of the sharpnose stingray (Dasyatis zugei). Isolated
ASC and PSC were subjected to chemical and physical characterizations. The yield of
PSC (34.84±1.26%) was significantly higher than that of ASC (20.48±4.41%) (p<0.05).
There were no significant differences between ASC and PSC in terms of chemical
composition (p>0.05). Both ASC and PSC were thermally stable at high temperatures,
with denaturation temperatures of 24.1°C and 25.2°C, respectively, and maximum
temperatures of 31.94±0.13°C and 31.79±0.23°C, respectively. Fourier transform infrared
(FTIR) investigations showed the presence of triple helical structure with strong hydrogen
bonding in both ASC and PSC. Meanwhile, both collagens were highly solubilized at
acidic pH but at different optimal pH. The surface morphologies of ASC and PSC were
loose and possessed slender, less uniform and irregular fibrous network structures with
large and irregular pores observed between the fibrils. This finding showed that the
alternative source of marine collagen possesses good physicochemical properties which
highly potential for nutraceutical, pharmaceutical or cosmeceutical application.
Isolation and characterization of microsatellite markers in the African catfish Clarias gariepinus (Burchell, 1822)