scholarly journals Characterization of Allele Diversity in a Microsatellite Locus: A Registry for <i>Solenopsis invicta</i>

2016 ◽  
Vol 04 (01) ◽  
pp. 32-36
Author(s):  
Rodrigo Fernando de Souza ◽  
Fernando Kamimura Cocchi ◽  
Cíntia Martins ◽  
Maria Santina de Castro Morini ◽  
Odair Correa Bueno

2013 ◽  
Vol 67 (3) ◽  
pp. 431-439 ◽  
Author(s):  
Steven M. Valles ◽  
DeWayne Shoemaker ◽  
Yannick Wurm ◽  
Charles A. Strong ◽  
Laura Varone ◽  
...  


2001 ◽  
Vol 47 (6) ◽  
pp. 543-551 ◽  
Author(s):  
Danielle K Lewis ◽  
Jon Q Campbell ◽  
Sheila M Sowa ◽  
Mei-Er Chen ◽  
S.B Vinson ◽  
...  


1997 ◽  
Vol 87 (3) ◽  
pp. 313-318 ◽  
Author(s):  
F. Vanlerberghe-Masutti ◽  
P. Chavigny

AbstractPrimers for DNA amplification using the polymerase chain reaction (PCR) were synthesized for a microsatellite locus isolated from a partial genomic library of the aphid parasitoid Aphelinus abdominalis (Dalman). Screening for genetic polymorphism at this locus in two laboratory strains of this wasp revealed the presence of two alleles different in the number of (GT) and (GGC) repeats. The relative frequencies of the two alleles were not significantly different between the two strains or between diploid females and haploid males. Heterozygosity at this microsatellite locus was estimated to be 0.40 which is within the range in other hymenopterous species. Given that A. abdominalis is a good candidate for augmentative release programmes in greenhouses against aphids, we suggest that microsatellite markers may have application in discriminating among aphelinid sibling species and strains. The markers provide a means for studying the performance and impact of selected parasitoid lines on pest dynamics in field release experiments.



HortScience ◽  
2010 ◽  
Vol 45 (8) ◽  
pp. 1286-1287 ◽  
Author(s):  
Long-na Li ◽  
Songjun Zeng ◽  
Feng Zheng ◽  
Zhi-lin Chen ◽  
Kun-lin Wu ◽  
...  

Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.





2008 ◽  
Vol 10 (5) ◽  
pp. 1373-1376 ◽  
Author(s):  
Zeng-Qiang Qian ◽  
Y. Ching Crozier ◽  
Birgit C. Schlick-Steiner ◽  
Florian M. Steiner ◽  
Ross H. Crozier


1993 ◽  
Vol 30 (6) ◽  
pp. 555-559 ◽  
Author(s):  
P. M. Rakich ◽  
K. S. Latimer ◽  
M. E. Mispagel ◽  
W. L. Steffens

Four adult dogs received experimentally controlled stings in the dorsolateral abdominal skin by imported fire ants ( Solenopsis invicta). The sites were examined grossly 15 minutes and at 1, 2, 3, 4, 5, 6, 24, 48, and 72 hours and histologically 15 minutes and 6, 24, 48, and 72 hours after stinging. The initial gross lesions at 15 minutes were swelling and erythema, and the microscopic changes were vascular congestion and superficial dermal edema. By 6 hours, the lesions consisted of bright erythematous pruritic papules characterized microscopically by a band of full thickness dermal necrosis and inflammation. By 24 hours and continuing to the end of the study at 72 hours, the sites appeared completely normal grossly. Biopsies taken 24, 48, and 72 hours after stings contained microscopic changes similar to those present at 6 hours after stings. These histologic changes are unlike those described for human beings stung by imported fire ants. In human beings, fire ant stings are characterized histologically by an initial superficial vesicle that evolves into a sterile pustule.



2008 ◽  
Vol 136 (1-2) ◽  
pp. 189-191 ◽  
Author(s):  
Steven M. Valles ◽  
Yoshifumi Hashimoto


Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.



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