scholarly journals A stage-scanning laser confocal microscope and protocol for DNA methylation sequencing

2010 ◽  
Vol 03 (05) ◽  
pp. 496-500
Author(s):  
Vaithilingam Vaishnavi ◽  
Litty Varghese ◽  
Baquir Mohammed Jaffar Ali
Keyword(s):  
Dna Methylation ◽  
Confocal Microscope ◽  
Scanning Laser ◽  
Laser Confocal Microscope ◽  
Stage Scanning

High-Qe Photodetector for Laser Confocal

1998 ◽  
Vol 4 (S2) ◽  
pp. 418-419
Author(s):  
J. Pawley ◽  
M. Blouke ◽  
J. Janesick
Keyword(s):  
Laser Power ◽  
Materials Science ◽  
Confocal Microscope ◽  
Data Rate ◽  
Research Tool ◽  
Biological Applications ◽  
Fluorescent Marker ◽  
The Past ◽  
Optical Photon ◽  
Laser Confocal Microscope

The laser confocal microscope (LCM) is now an established research tool in biology and materials science. In biological applications, it is usually employed to detect the location of fluorescent marker molecules and, under these conditions, detected signal levels from bright areas often represent <20 photons/pixel (assuming a standard 1.6 μs pixel time) while those from dark areas are likely to average <1 photon/pixel. Although this data rate limits the speed at which information can be derived from the specimen, saturation of the fluorophor, photobleaching of the dye, and phototoxicity often prevent it being increased by simply using more laser power. Over the past 10 years, the optical photon efficiency of commercial confocal instruments has improved significantly and it is now reaching the point where further improvement is becoming very expensive. The only component is which a significant improvement is still possible is the photodetector.

Line-scanning, stage scanning confocal microscope

2016 ◽  
Author(s):  
John A. Carucci ◽  
Mary Stevenson ◽  
Daniel Gareau
Keyword(s):  
Confocal Microscope ◽  
Line Scanning ◽  
Stage Scanning

Laser confocal microscope with wavelet-profiled point spread function

2010 ◽  
Vol 283 (7) ◽  
pp. 1217-1221
Author(s):  
Mary Jacquiline Romero ◽  
Godofredo Bautista ◽  
Vincent Ricardo Daria ◽  
Caesar Saloma
Keyword(s):  
Point Spread Function ◽  
Confocal Microscope ◽  
Point Spread ◽  
Spread Function ◽  
Laser Confocal Microscope

Influence of Load and Rotation Speed on Life of PPS Radial Bearings under Water Lubricant Conditions

2013 ◽  
Vol 683 ◽  
pp. 439-443 ◽  
Author(s):  
Shunsuke Oyama ◽  
Hitonobu Koike ◽  
Yuji Kashima ◽  
Katsuyuki Kida
Keyword(s):  
Rotation Speed ◽  
Confocal Microscope ◽  
Rolling Contact ◽  
Contact Surfaces ◽  
Laser Confocal Microscope ◽  
Water Lubricant

Influence of load and rotation speed on life of PPS radial bearings under water lubricant condition was investigated. RCF tests were carried out at different loads and rotation speeds until 1.0×106 cycles. After testing, rolling contact surfaces were observed by using a laser confocal microscope. From the observation, it was concluded that the life of PPS radial bearings under water lubricant condition was dominated by cracks and flaking failures. The failure of PPS bearings was caused by the flaking failures. The cracks were observed prior to the flaking failures. The life when flaking failure occur depends on the load and the rotation speed.

An Evaluation of Decorative Techniques on a Red-Figure Attic Vase from the Worcester Art Museum using Reflectance Transformation Imaging (RTI) and Confocal Microscopy with a Special Focus on the “Relief Line”

2011 ◽  
Vol 1319 ◽  
Author(s):  
Paula Artal-Isbrand ◽  
Philip Klausmeyer ◽  
Winifred Murray
Keyword(s):  
Confocal Microscopy ◽  
Art Museum ◽  
Confocal Microscope ◽  
Scanning Laser ◽  
Special Focus ◽  
Laser Confocal Microscopy ◽  
Ancient Technology ◽  
Surface Examination ◽  
Tools And Techniques ◽  
Reflectance Transformation Imaging

ABSTRACTDecorative features on a Greek red-figure stamnos in the collection of the Worcester Art Museum were examined using Reflectance Transformation Imaging (RTI) and scanning laser confocal microscopy. These two surface examination tools helped to answer questions relating to the decorative process, particularly the tools and techniques that Attic painters used to create the so-called glossy black “relief lines” and “relief dots.” This research also incorporated fabricated mock-ups to help understand the ancient technology. It was determined that the relief line was not produced by an extruded method, but with a brush made of one or very few hairs, an idea first proposed by Gérard Seiterle in 1976 and termed Linierhaar. It was observed that not one but two distinct types of relief lines exist: the “laid” line (proposed by Seiterle) characterized by a ridge running through the middle of the line and the “pulled” line (proposed in this paper) which has a furrowed profile. Both line types were reproduced with a Linierhaar. Additionally, relief dots were replicated using a conventional brush. Surface examinations of other red-figure vessels using RTI and the confocal microscope suggest these conclusions apply to vessels of this genre as a whole.

Sign in / Sign up

Export Citation Format

Share Document