Litter decomposition is a fundamental nutrient cycling process, and
litter diversity decreases induced by biodiversity loss have substantial
effects on soil carbon cycling. However, few experimental studies have
characterized the effect of litter diversity on and litter chemistry.
Here, we used single-species and mixed litters to study the effects of
litter chemical properties on the direction, intensity and drivers of
non-additive litter-mixing effects. We found that 1) there was no
significant effect of litter species richness on soil processes, and the
litter chemistry of component species was more robust to soil
respiration and non-additive effects. 2) The early-stage mixing effect
was negative, ranging from -3.1 to -0.3, and its magnitude was strongest
in chemically diverse litter mixtures; the late-stage mixing effect
ranged from -2.3 to 1.3, and the non-additive effect of chemically
similar species was positive. 3) Litter carbon, lignin, phenols and
soluble sugar affected early-stage soil respiration, and litter carbon,
nitrogen, phenols, and condensed tannins affected late-stage soil
respiration, which accounted for 46% and 56% of the variation in
early- and late-stage soil respiration, respectively. 4) Compared with
plant species richness, litter chemistry altered the direction and
magnitude of litter mixing, and litter chemical composition (including
litter chemical traits and their interactions) had a stronger effect on
non-additive effects than variation in single chemical compounds
according to the R value (R=0.36).
5) Artemisia halodendron, as a key sand-fixing plant species, will
accelerate nutrient cycling, but it has negative effects on carbon
cycling when mixed with other plant species