A simple method for diagnosing Phytophthora rot of strawberry caused by Phytophthora nicotianae var. parasitica

1980 ◽  
Vol 26 ◽  
pp. 45-48
Author(s):  
Masafumi MATSUZAKI ◽  
Masamiti KAN
2006 ◽  
Vol 22 (3) ◽  
pp. 211-214 ◽  
Author(s):  
Jin-Hyeuk Kwon ◽  
Hyeong-Jin Jee

2004 ◽  
Vol 20 (4) ◽  
pp. 235-239
Author(s):  
Hyeong-Jin Jee ◽  
Shun-Shan Shen ◽  
Chang-Seuk Park ◽  
Jin-Hyeuk Kwon

2007 ◽  
Vol 23 (1) ◽  
pp. 31-33
Author(s):  
Jin-Hyeuk Kwon ◽  
Hyeong-Jin Jee ◽  
Shun-Shan Shen ◽  
Yun-Seok Chae

2012 ◽  
Vol 29 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Abbas Mohammadi

Abstract A high detection rate of Phytophthora nicotianae was obtained from the naturally infested soil of soybean fields. Air-dried soils were firstly moistened in a flask and then pre-incubated at 25 °C for 1-4 weeks before flooding with distilled water and baiting with soybean leaf disks for 6, 12, and 24 h. The baits were then thoroughly washed, flooded with 10-15 ml of distilled water in Petri-dishes and incubated under continuous fluorescent light for 72 h. Sporangia started to emerge from the margins of leaf disks. They were easily observed under the stereomicroscope. Pure culture of the fungus was obtained by spreading zoospore suspension on a 1.5% water-agar containing anti-bacterial antibiotics. This is the first report of recovery of P. nicotianae from naturally infested soybean soils using sensitive leaf disks of soybean (Williams cultivar) as bait in Iran. All isolates were determined to be A2 mating type.


1980 ◽  
Vol 46 (2) ◽  
pp. 169-178 ◽  
Author(s):  
Takahito SUZUI ◽  
Tokio MAKINO ◽  
Akira OGOSHI

Author(s):  
K.-H. Herrmann ◽  
E. Reuber ◽  
P. Schiske

Aposteriori deblurring of high resolution electron micrographs of weak phase objects can be performed by holographic filters [1,2] which are arranged in the Fourier domain of a light-optical reconstruction set-up. According to the diffraction efficiency and the lateral position of the grating structure, the filters permit adjustment of the amplitudes and phases of the spatial frequencies in the image which is obtained in the first diffraction order.In the case of bright field imaging with axial illumination, the Contrast Transfer Functions (CTF) are oscillating, but real. For different imageforming conditions and several signal-to-noise ratios an extensive set of Wiener-filters should be available. A simple method of producing such filters by only photographic and mechanical means will be described here.A transparent master grating with 6.25 lines/mm and 160 mm diameter was produced by a high precision computer plotter. It is photographed through a rotating mask, plotted by a standard plotter.


Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.


2010 ◽  
Vol 34 (8) ◽  
pp. S75-S75
Author(s):  
Weifeng Zhu ◽  
Zhuoqi Liu ◽  
Daya Luo ◽  
Xinyao Wu ◽  
Fusheng Wan

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