Optimization of supercritical fluid extraction of bioactive components in Ligusticum chuanxiong by orthogonal array design

2013 ◽  
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Orthogonal Array ◽  
Orthogonal Array Design ◽  
Bioactive Components ◽  
Fluid Extraction ◽  
Array Design ◽  
Ligusticum Chuanxiong

scholarly journals Supercritical Fluid Extraction of Pyrrolidine Alkaloid from Leaves of Piper amalago L.

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
V. S. Carrara ◽  
L. C. Filho ◽  
V. A. S. Garcia ◽  
V. S. Faiões ◽  
E. F. Cunha-Júnior ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Orthogonal Array ◽  
Design Matrix ◽  
Supercritical Carbon Dioxide Extraction ◽  
Orthogonal Array Design ◽  
Fluid Extraction ◽  
Array Design ◽  
Carbon Dioxide Extraction

Supercritical fluid extraction was used to extract the alkaloid N-[7-(3′,4′-methylenedioxyphenyl)-2(Z),4(Z)-heptadienoyl]pyrrolidine from leaves of Piper amalago L. A three-level orthogonal array design matrix, OAD OA9(34), was used for optimization of the parameters of supercritical extraction of the alkaloid, employing supercritical carbon dioxide: extraction time (20, 40, and 60 min), temperature (40, 50, and 60°C), pressure (150, 200, and 250 bar), and the use of cosolvents (ethanol, methanol, and propyleneglycol). All parameters had significant effect on the alkaloid yield. The alkaloid yield after 60 min of extraction without cosolvents at 9 different conditions (32) in terms of temperature (40, 50, and 60°C) and pressure (150, 200, and 250 bar) was also evaluated. The optimal yield (≈3.8 mg g−1) was obtained with supercritical CO2 + methanol (5% v : v) at 40°C and 200 bar for 60 min of extraction.

Bioactive Component Extraction from Antrodia camphorata Fruiting Body on Artificial Agar Media

2013 ◽  
Vol 750-752 ◽  
pp. 1485-1488
Author(s):  
Wen Lu Weng ◽  
Hao Min Lo ◽  
Shih Jung Chan ◽  
Wen Huang Liu
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Bioactive Components ◽  
Fluid Extraction ◽  
Antrodia Camphorata ◽  
Working Pressure ◽  
Operation Temperature ◽  
Bioactive Component ◽  
Agar Media ◽  
Optimum Extraction

Supercritical fluid extraction (carbon dioxide), water extraction and ethanol extraction are used to extract bioactive components from fruiting bodies of Antrodia Camphorata to find out the optimum extraction condition through operation variable changes. Analysis comparisons tell that the best condition for water and ethanol approaches is 95% ethanol as the solvent, 45 °C as the operation temperature and 24 hours for extraction while supercritical fluid extraction prefers 95 % ethanol as the co-solvent, 250 bar as the working pressure and 45 °C as the operation temperature. There exist more and significant low polarity peaks in HPLC detection chromatograms for supercritical fluid extract liquids.

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