Use of Non-Human Plasma for in Vitro Cultivation and Antimalarial Drug Susceptibility Testing of Plasmodium Falciparum

1985 ◽  
Vol 34 (2) ◽  
pp. 209-215 ◽  
Author(s):  
Ayoade M. J. Oduola ◽  
Robert E. Desjardins ◽  
Bruce M. Alexander ◽  
Norman F. Weatherly ◽  
Jean H. Bowdre
Keyword(s):  
Plasmodium Falciparum ◽  
Human Plasma ◽  
Antimalarial Drug ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
In Vitro Cultivation

scholarly journals Antimalarial drug susceptibility testing of Plasmodium falciparum in Brazil using a radioisotope method

1999 ◽  
Vol 94 (6) ◽  
pp. 803-809 ◽  
Author(s):  
Crispim Cerutti Junior ◽  
Christiane Marques ◽  
Filomena EC de Alencar ◽  
Rui Rafael Durlacher ◽  
Anna Alween ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antimalarial Drug ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Radioisotope Method

Growth, Drug Susceptibility, and Gene Expression Profiling of Plasmodium falciparum Cultured in Medium Supplemented with Human Serum

2007 ◽  
Vol 12 (8) ◽  
pp. 1109-1114 ◽  
Author(s):  
Kshipra Singh ◽  
Ameeta Agarwal ◽  
Shabana I. Khan ◽  
Larry A. Walker ◽  
Babu L. Tekwani
Keyword(s):  
Gene Expression ◽  
Plasmodium Falciparum ◽  
Human Serum ◽  
Global Gene Expression ◽  
Drug Susceptibility ◽  
Growth Cycle ◽  
In Vitro Cultivation ◽  
Human Malaria Parasite ◽  
In Vitro Drug Susceptibility

In vitro cultivation of Plasmodium falciparum has been extremely useful in understanding the biology of the human malaria parasite as well as research on the discovery of new antimalarial drugs and vaccines. A chemically defined serum-free medium supplemented with lipid-rich bovine serum albumin (AlbuMAX I) offers the following advantages over human serum-supplemented media for the in vitro culture of P. falciparum: 1) improved growth profile, with more than a 2-fold higher yield of the parasites at any stage of the growth cycle; 2) suitability for in vitro antimalarial screening, as the parasites grown in AlbuMAX and human serum-supplemented media show similar sensitivity to standard and novel antimalarials as well as natural product extracts in the in vitro drug susceptibility assays; and 3) DNA microarray analysis comparing the global gene expression profile of sorbitol-synchronized P. falciparum trophozoites grown in the 2 different media, indicating minimal differences. ( Journal of Biomolecular Screening 2007:1109-1114)

Use of HIV-1 reverse transcriptase recovered from human plasma for phenotypic drug susceptibility testing

AIDS ◽  
2003 ◽  
Vol 17 (10) ◽  
pp. 1463-1471 ◽  
Author(s):  
Xing-Wu Shao ◽  
Anders Malmsten ◽  
Johan Lennerstrand ◽  
Anders Sönnerborg ◽  
Torsten Unge ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Human Plasma ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Hiv 1

scholarly journals Pyrazinamide Is Active against Mycobacterium tuberculosis Cultures at Neutral pH and Low Temperature

2016 ◽  
Vol 60 (8) ◽  
pp. 4956-4960 ◽  
Author(s):  
Alice L. den Hertog ◽  
Sandra Menting ◽  
Richard Pfeltz ◽  
Matthew Warns ◽  
Salman H. Siddiqi ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Low Temperature ◽  
Susceptibility Testing ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Acidic Ph ◽  
Content Type ◽  
The Past ◽  
Neutral Ph

ABSTRACTFor the past decades, an acidic pH has been used to renderMycobacterium tuberculosissusceptible to pyrazinamide forin vitrotesting. Here, we show that at the standard breakpoint concentration and reduced culture temperatures, pyrazinamide (PZA) is active against tuberculosis (TB) at neutral pH. This finding should help unravel the mechanism of action of PZA and allow drug susceptibility testing (DST) methods to be optimized.

scholarly journals In vitro evaluation of verapamil and other modulating agents in Brazilian chloroquine-resistant Plasmodium falciparum isolates

2003 ◽  
Vol 36 (1) ◽  
pp. 5-9 ◽  
Author(s):  
Carla M.S. Menezes ◽  
Karin Kirchgatter ◽  
Sílvia M. Di Santi ◽  
Carine Savalli ◽  
Fabíola G. Monteiro ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antipsychotic Drug ◽  
Antimalarial Drug ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
Antiplasmodial Activity ◽  
Experimental Conditions ◽  
In Vitro Evaluation ◽  
Cardiovascular Therapy

Verapamil, was assayed to record its modulating effect upon Brazilian Plasmodium falciparum isolates resistant to chloroquine. Other cardiovascular drugs known to be modulating agents in resistant malaria and/or multidrug-resistant neoplasias, including nifedipine, nitrendipine, diltiazem and propranolol, were also evaluated. Concentrations similar to those for cardiovascular therapy were used in the in vitro microtechnique for antimalarial drug susceptibility. Intrinsic antiplasmodial activity was observed from the lowest concentrations without a significant modulating action. Other reported modulating agents, such as the antipsychotic drug trifluoperazine and the antidepressants desipramine and imipramine, demonstrated similar responses under the same experimental conditions. Results suggest a much higher susceptibility of Brazilian strains, as well as an indifferent behaviour in relation to modulating agents.

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