scholarly journals Risk evaluation of industrial wastewater on plants using onion(Allium cepa l) chromosome aberration assay

Agro-Science ◽  
2009 ◽  
Vol 7 (3) ◽  
Author(s):  
AE Ngozi ◽  
SC Ezeugwu
Keyword(s):  
Chromosome Aberration ◽  
Allium Cepa ◽  
Risk Evaluation ◽  
Industrial Wastewater ◽  
Chromosome Aberration Assay ◽  
Allium Cepa L

Genotoxicity Screening of Industrial Wastewater Using the Allium cepa Chromosome Aberration Assay

2002 ◽  
Vol 6 (1) ◽  
pp. 23-28 ◽  
Author(s):  
O.A. El-Shahaby ◽  
H.M. Abdel Migid ◽  
M.I. Soliman . ◽  
I.A. Mashaly .
Keyword(s):  
Chromosome Aberration ◽  
Allium Cepa ◽  
Industrial Wastewater ◽  
Chromosome Aberration Assay

scholarly journals Influence of 1-p-D-arabinofuranosylcytosine on mitotic activity of apical meristem of onion (Allium cepa L.) roots

2015 ◽  
Vol 47 (1–2) ◽  
pp. 173-187 ◽  
Author(s):  
M. Kuraś ◽  
A. Malinowska
Keyword(s):  
Mitotic Activity ◽  
Chromosome Aberration ◽  
Allium Cepa ◽  
Cytosine Arabinoside ◽  
Adventitious Roots ◽  
Apical Meristem ◽  
Allium Cepa L ◽  
Cells With Micronuclei

The influence of increasing cytosine arabinoside (ara-C) concentration (50, 100, 300 and 500 μg/ml) on the mitotic activity of the apical meristem of onion adventitious roots was investigated during 24-h incubation in ara-C and postincubation in water. Incubation in ara-C inhibits reversibly mitosis, the degree of inhibition being dependent on the concentration used. 50 μg/ml ara-C causes only a slight and transitional mitotic depression, while 100—500 μg/ml reduces the per cent of mitoses in various degrees after 12-h incubation whereas after 24 h all concentrations (100—500) reduce mitosis to the same level of about 2 per cent (80% inhibition). During postincubation in water there occurs after treatment with 100—500 μg/ml ara-C, a wave of highly synchronized mitoses with a peak after 12 h. The highest frequency of synchronized mitoses (60°/o) appeared in roots treated with 300 μg/ml ara-C. The main wave is preceded by a small one with a peak after 2-4 h of postincubation. This seems to be the consequence of phases S and G<sub>2</sub> inhibition by ara-C. It was found that under the influence of ara-C the per cent of mitoses with chromosome aberration and of cells with micronuclei increased only slightly.

scholarly journals Cytogenetic effects of insecticide Lamda cyhalothrin on meristemic cells of Allium cepa L.

2008 ◽  
Vol 5 (2) ◽  
pp. 166-172
Author(s):  
Baghdad Science Journal
Keyword(s):  
Mitotic Index ◽  
Chromosome Aberration ◽  
Allium Cepa ◽  
Cytogenetic Effects ◽  
Binucleated Cells ◽  
Allium Cepa L ◽  
Spindle Fibers

The effect of insecticide lamda cyhalothrin on Allium cepa L.root meristem were studied cytogenetically .Using three concentrations of the insecticide 5%, 7.5%, 10% at 6,12,24hours treatments . The insecticide significantly reduced the mitotic index at all concentrations. Moreover showed its ability to induced C – metaphase in 10% .The effects were also caused chromosome aberration like stickness in anaphase, telophase, binucleated cells, Micronuclei formation. These alternations indicating that this insecticide was effective in producing disturbance of spindle fibers.

scholarly journals Cytogenetic effect of crude aqueous extract from leaf of Lycium barbarum on root tip of Allium cepa L.

2008 ◽  
Vol 5 (4) ◽  
pp. 492-499
Author(s):  
Baghdad Science Journal
Keyword(s):  
Mitotic Index ◽  
Chromosome Aberration ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Sublethal Effect ◽  
Root Tip ◽  
Lycium Barbarum ◽  
Phase Index ◽  
Allium Cepa L ◽  
Crude Aqueous Extract

This study was carried out to investigate the cytogenetic effects of crude aqueous extract of Lycium barbarum on the roots tip of Allium cepa Using three concentration 125, 25, 50 mg/ ml for 2, 4, 6 hours treatment periods.This study were included some cytogenetic analysis such as mitotic index , phase index and chromosome aberration. The data showed that the treatment with 50mg/ml for 6huors led to reduce the mitotic index less than 50% . This reduction considered to have toxic and sublethal effect . These results revealed mutagenic potency by inducing differents type of chromosome aberration.

scholarly journals ECOTOXICOLOGICAL RISK EVALUATION OF HERBICIDES ON NON-TARGET ENVIRONMENTAL RECEPTORS

2020 ◽  
Vol 4 (2) ◽  
pp. 92-99
Author(s):  
Felix Ebodaghe Okieimen. ◽  
Doris Fovwe Ogeleka ◽  
Beatrice Oghenetega Peretiemo -Clarke
Keyword(s):  
Risk Assessment ◽  
High Risk ◽  
Allium Cepa ◽  
Risk Evaluation ◽  
Effective Concentration ◽  
Risk Level ◽  
Target Species ◽  
Ecotoxicological Risk ◽  
Allium Cepa L ◽  
Assessment Matrix

Globally, with the application of over 4 billion tons of herbicides yearly to suppress or kill weeds, the uncontrollable / unregulated use and disposal of these sometimes-recalcitrant chemicals could result in deleterious effects on non-target ecological receptors. This evaluation recorded varying degree of toxic effects on the environmental receptors (onions – Allium cepa L) and (snails – Archachatina marginata). The highest effective concentration (EC50) for onions was recorded in Dragon® (0.042 ± 0.008 mg L-1) while the lowest effective concentration was in Uproot® (2.185 ± 0.52 mg L-1). Similarly, the highest EC50 in the snail test was obtained in Cotrazine® (0.41 ± 0.012 mg kg-1) and the lowest in Striker® (9.51 ± 0.94 mg kg-1). The risk level estimated using the Ecotoxicological Risk Assessment Matrix (ERAM) revealed that the least toxic herbicide were rated E 3 (P;A;E;C) or 15 (P;A;E;C) while the most toxic herbicides were rated E 5 (P;A;E;C) or 25 (P;A;E;C), which was considered medium to high risk to plants (P), animal (A), the environment (E) and community (C). The use, handling and disposal of these lethal herbicides should be prudently managed so as to safe guide non-target species in the environment of application.

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